Products
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月7日11時30分 現在 |
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| #9621S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| #9621L | 300 μL | 137,000 |
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9621 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫沈降 (1:100)、免疫組織染色 (パラフィン) (1:200)、ELISA-P (1:500) |
| 種交差性 | |
|---|---|
| すべての種 |
| 特異性・感度 | |
|---|---|
| -3 の位置にアルギニンが存在するリン酸化スレオニン、リン酸化セリンを検出します。リン酸化されていないPKA 基質モチーフとは交差しません。 |
| 使用抗原 | |
|---|---|
| リン酸化PKA の基質ペプチド |
| モチーフ | |
|---|---|
| (K/R)(K/R)X(S*/T*) |
| 抗体の由来 | |
|---|---|
| ウサギ |
| 貯法 | |
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| -20℃ |
| License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commerical licensing terms please contact at info@cstj.co.jp. |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser/Thr) PKA Substrate Antibody.
Western Blotting
Western blot analysis of extracts from A431 cells, phosphorylated in vitro by protein kinase A, Erk2 or cdc2/cyclin A, plus or minus PKA inhibitor (PKI), using Phospho-(Ser/Thr) PKA Substrate Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-(Ser/Thr) PKA Substrate Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-(Ser/Thr) PKA Substrate Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phsopho-(Ser/Thr) PKA Substrate Antibody.
ELISA-Peptide
Phospho-(Ser/Thr) PKA Substrate Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.)
| バックグラウンド |
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An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.
| 使用文献 |
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- Lei, H. et al. (2007) J Biol Chem 282, 9364-71. Applications: Western Blotting
- Marcinkiewicz, A. et al. (2006) J Biol Chem 281, 11901-9. Applications: Western Blotting
- Su, Y.H. and Vacquier, V.D. (2006) Mol Biol Cell 17, 114-21. Applications: Western Blotting
- Miyoshi, H. et al. (2006) J Biol Chem 281, 15837-44. Applications: Western Blotting
- Smith, J.A. et al. (2005) Cancer Res 65, 1027-34. Applications: Western Blotting
- Wada, S. et al. (2005) J Biol Chem 280, 94-103. Applications: Western Blotting
- Hatakeyama, M. et al. (2004) Oncogene 23, 5023-31. Applications: Western Blotting
- Schmitt, J.M. and Stork, P.J. (2002) Mol Cell 9, 85-94. Applications: Western Blotting
- Grønborg, M. et al. (2002) Mol Cell Proteomics 1, 517-27. Applications: IP Western Blotting
- Bruce, J.I. et al. (2002) J Biol Chem 277, 1340-8. Applications: IP Western Blotting
- Schmitt, A. and Nebreda, A.R. (2002) Proc Natl Acad Sci U S A 99, 4361-6. Applications: Western Blotting
本製品は試験研究用です。