Tyrosine Kinases / Adaptors
- Tyrosine Kinases / Adaptors
PhosphoSitePlus®：
14-3-3 theta
- 推奨プロトコール
14-3-3 τ Antibody |
イイネ！(0) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日11時35分 現在 |
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| #9638S | 100 μL | 46,000 | ログインすると国内在庫状況がご確認いただけます。
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9638 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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下記ステップについては、データシートの右側もあわせてご参照ください。
IHC-P: 抗体希釈液 / 抗原賦活化
| 用途 (希釈倍率) | |
|---|---|
| ウェスタンブロッティング (1:1,000)、免疫組織染色 (パラフィン) (1:25) |
| 種交差性 | |
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| ヒト、マウス、ラット、サル |
| 特異性・感度 | |
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| 内在性レベルの14-3-3 τタンパク質を検出します。 |
| 検出タンパク質の分子量 | |
|---|---|
| 28 kDa |
| 使用抗原 | |
|---|---|
| ヒトの14-3-3 τタンパク質の配列 (合成ペプチド) |
| 抗体の由来 | |
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| ウサギ |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human GIST, using 14-3-3 tau Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using 14-3-3 tau Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody in the presence of control peptide (left) or antigen-specific peptide (right).
| バックグラウンド |
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The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions, has been observed (4). 14-3-3 binding masks specific sequences of the target protein and therefore modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).
| 使用文献 |
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- Lee, J.W. et al. (2010) PLoS One 5, e15394. Applications: Western Blotting
本製品は試験研究用です。