Wnt / Hedgehog / Notch

StemLight™ Pluripotency Antibody Kit

 イイネ！(0) StemLight™ Pluripotency Antibody Kit Data Sheet PDF
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2012年2月9日11時30分 現在
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#9656S1 Kit126,000
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Stem Cell Kit抗体製品一覧

キット内容 容量 用途 希釈率 Isotype
Oct-4A (C30A3) Rabbit mAb 100 tests IF-IC 1:200 Rabbit IgG
Sox2 (D6D9) XP® Rabbit mAb 100 tests IF-IC 1:200 Rabbit IgG
Nanog Antibody 100 tests IF-IC 1:200 Rabbit IgG
SSEA4 (MC813) Mouse mAb 100 tests IF-IC 1:200 Mouse IgG3
TRA-1-60(S) (TRA-1-60(S)) Mouse mAb 100 tests IF-IC 1:200 Mouse IgM
TRA-1-81 (TRA-1-81) Mouse mAb 100 tests IF-IC 1:200 Mouse IgM
貯法
-20℃
社内データ

IF-IC

IF-IC

Projected confocal z-stack of human iPS cells using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green, upper right), TRA-1-81 (TRA-1-81) Mouse mAb (green, upper middle), SSEA4 (MC813) Mouse mAb (green, upper left), Oct-4A (C30A3) Rabbit mAb (green, lower right), Sox2 (D6D9) XP® Rabbit mAb (green, lower middle) and Nanog Antibody (green, lower left). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells, untreated (left panel) or retinoic acid-treated (10 µM all-trans RA for 5 days) (right panel), using TRA-1-60(S) (TRA-1-60(S)) Mouse mAb (green, upper), TRA-1-81 (TRA-1-81) Mouse mAb (green, middle) and SSEA4 (MC813) Mouse mAb (green, lower). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note the loss of pluripotency markers (green) as cells differentiate along the neuronal lineage with retinoic acid treatment.

IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells, untreated (left panel) or retinoic acid-treated (10 µM all-trans RA for 14 days) (right panel), using Oct-4A (C30A3) Rabbit mAb (green, upper), Sox2 (D6D9) XP® Rabbit mAb (green, middle) and Nanog Antibody (green, lower). Actin filaments have been labeled with DY-554 phalloidin (red). Note the loss of pluripotency markers (green) as cells differentiate along the neuronal lineage with retinoic acid treatment.


IF-IC

IF-IC

Confocal immunofluorescent analysis of NTERA-2 cells, untreated (left panel) or retinoic acid-treated (10 µM all-trans RA for 5 days) (right panel), using Neurofilament-L (C28E10) Rabbit mAb #2837 (green, upper), and β3-Tubulin (TU-20) Mouse mAb #4466 (green, lower). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note the appearance of neuronal markers and structures as cells differentiate along the neuronal lineage with retinoic acid treatment.

バックグラウンド

Pluripotency is the ability of a cell to differentiate into cell types of the three germ layers, the endoderm, ectoderm and mesoderm. It is a property shared by embryonic stem cells, embryonic carcinoma and induced pluripotent cells.Oct-4, Sox2 and Nanog are key transcriptional regulators that are highly expressed in pluripotent cells (1). Together they form a transcriptional network that maintains cells in a pluripotent state (2,3). Over-expression of Oct-4 and Sox2 along with Klf4 and c- Myc can induce pluripotency in both mouse and human somatic cells, highlighting their roles as key regulators of the transcrip- tional network necessary for renewal and pluripotency (4-5). It has also been demonstrated that overexpression of Oct-4, Sox2, Nanog and Lin28 can induce pluripotency in human somatic cells (6). Upon differentiation of pluripotent cultures, expression of Oct-4, Nanog and Sox2 is downregulated.SSEA4, TRA-1-81 and TRA-1-60 antibodies recognize antigens expressed on the cell surface of all pluripotent cells. SSEA4 recognizes a glycolipid carbohydrate epitope (7). TRA-1-60(S) and TRA-1-81 antibodies recognize different proteoglycan epitopes on variants of the same protein, podocalyxin (8). These epitopes are neurominadase sensitive and resistant, respectively. Reactivity of SSEA4, TRA-1-81 and TRA-1-60 antibodies with their respective cell surface markers are lost upon differentiation of pluripotent cells, corresponding with a loss of pluripotent potential (9).

  1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
  5. Okita, K. et al. (2007) Nature 448, 313-7.
  6. Yu, J. et al. (2007) Science 318, 1917-20.
  7. Henderson, J.K. et al. (2002) Stem Cells 20, 329-37.
  8. Draper, J.S. et al. (2002) J Anat 200, 249-58.
  9. Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30.
使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。
 
関連製品
2837   Neurofilament-L (C28E10) Rabbit mAb
2840   Oct-4A (C30A3) Rabbit mAb
3579   Sox2 (D6D9) XP® Rabbit mAb
3580   Nanog Antibody
3695   LIN28A (D84C11) XP® Rabbit mAb
3978   LIN28A (A177) Antibody
4038   KLF4 Antibody
4084   DRAQ5®
4408   Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
4409   Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
4410   Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
4412   Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
4413   Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
4414   Anti-Rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
4466   β3-Tubulin (TU-20) Mouse mAb
4745   TRA-1-81 (TRA-1-81) Mouse mAb
4746   TRA-1-60(S) (TRA-1-60(S)) Mouse mAb
4753   Cellular Localization IF Antibody Sampler Kit
4755   SSEA4 (MC813) Mouse mAb
5605   c-Myc (D84C12) XP® Rabbit mAb
5930   LIN28A (6D1F9) Mouse mAb

本製品は試験研究用です。

StemLight™ Pluripotency Antibody Kit

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