Chromatin Regulation / Acetylation
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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年9月8日13時0分 現在 |
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| #9763S | 100 μL | 57,000 | ログインすると国内在庫状況がご確認いただけます。
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| 用途（希釈倍率） | |
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| ウェスタンブロッティング（1:1,000）、免疫組織染色（パラフィン）（1:50）、免疫蛍光細胞染色（IF-IC）（1:200） | |
| 種交差性 | |
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| ヒト、マウス、ラット、サル | |
| 特異性・感度 | |
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| 内在性レベルのLys36 がトリメチル化されたHistone H3 タンパク質を検出します。Lys36 がメチル化されていない場合、およびモノメチル化、ジメチル化されたHistone H3 タンパク質とは交差しません。更に、Lys4、Lys9、Lys27 がメチル化されたHistone H3、またはLys20 がリン酸化されたHistone H4 タンパク質と交差しません。 | |
| 検出タンパク質の分子量 | |
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| 17 kDa | |
| 使用抗原 | |
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| Lys36 がトリメチル化されたHistone H3 タンパク質のN末端領域（合成ペプチド） | |
| 抗体の由来 | |
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| ウサギ | |
| 貯法 | |
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| -20℃ | |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from various cell lines using Tri-Methyl-Histone H3 (Lys36) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Tri-Methyl-Histone H3 (Lys36) Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Tri-Methyl-Histone H3 (Lys36) Antibody in the presence of control peptide (left) or antigen specific peptide (right).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using Tri-Methyl-Histone H3 (Lys36) Antibody (green). Actin filaments have been labeled with DY-554 (red).
ELISA-Peptide
Tri-Methyl-Histone H3 (Lys36) Antibody specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated tri-methyl histone H3 (Lys36) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the tri-methyl histone H3 (Lys36) peptide competed away binding of the antibody.
| バックグラウンド |
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The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1) and WD-40 domains (WDR5) (5-8). The recent discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2 and JHDM1 has shown that methylation is a reversible epigenetic mark (9).
- Peterson, C.L. and Laniel, M.A. (2004) Curr. Biol. 14, R546-R551.
- Kubicek, S. et al. (2006) Ernst Schering Res. Found Workshop , 1-27.
- Lin, W. and Dent, S.Y. (2006) Curr. Opin. Genet. Dev. 16, 137-142.
- Lee, D.Y. et al. (2005) Endocr. Rev. 26, 147-170.
- Daniel, J.A. et al. (2005) Cell Cycle 4, 919-926.
- Shi, X. et al. (2006) Nature 442, 96-99.
- Wysocka, J. et al. (2006) Nature 442, 86-90.
- Wysocka, J. et al. (2005) Cell 121, 859-872.
- Trojer, P. and Reinberg, D. (2006) Cell 125, 213-217.
| 使用例 | |
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本製品は試験研究用です。
