Cell Cycle / Checkpoint Control
Cell Cycle Regulation Antibody Sampler Kit II |
イイネ！(1) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #9870S | 1 Kit | 115,000 | ログインすると国内在庫状況がご確認いただけます。
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9870 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 2947:
- Flow IHC / Paraffin Immunofluorescence Immunoprecipitation Western Blotting
- 3377:
- Flow Immunofluorescence Western Blotting
- 4132:
- Western Blotting
- 4138:
- Western Blotting
- 4282:
- IHC / Paraffin Western Blotting
- 4656:
- Flow Western Blotting
- 4910:
- Immunoprecipitation Western Blotting
- 9111:
- ELISA Peptide Immunoprecipitation Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| Phospho-cdc2 (Tyr15) Antibody #9111 | 40 µl | W IP | H M R Mk Dm X | 34 | Rabbit |
| Cyclin A (BF683) Mouse mAb #4656 | 40 µl | W F | H | 55 | Mouse IgG2b |
| Cyclin B1 Antibody #4138 | 40 µl | W IF-IC | H M R Hm Mk | 58 | Rabbit |
| Cyclin E2 Antibody #4132 | 40 µl | W | H | 48 | Rabbit |
| Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 | 40 µl | W IF-IC F | H M R Mk Z | 17 | Rabbit IgG |
| Myt1 Antibody #4282 | 40 µl | W IHC-P | H M R (X) | 60 to 70 | Rabbit |
| p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 | 40 µl | W IP IHC-P IF-IC F | H Mk | 21 | Rabbit IgG |
| Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb #4910 | 40 µl | W IP | H (M) (R) (Mk) (X) (Z) (B) | 95 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| Anti-mouse IgG, HRP-linked Antibody #7076 | 100 µl | Horse |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from various cell types using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or treated with nocodazole (0.1 mg/ml for 18 hours), using Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 (upper) or Histone H3 Antibody #9715 (lower). Phospho-specificity of the antibody is shown by further treatment of the lysate with λ phosphatase.
Western Blotting
Western blot analysis of extracts from MCF7, SK-N-MC and HeLa cells, untreated or treated with the proteasome inhibitor MG-132, using Cyclin E2 Antibody #4132.
Western Blotting
Western blot analysis of extracts from various cell types using Cyclin B1 Antibody #4138.
Western Blotting
Western blot analysis of extracts from HT-29 cells, untreated (lane 1), nocodazole-treated (50 ng/ml, lane 2), or UV-treated (lane 3), using Myt1 Antibody #4282.
Western Blotting
Western analysis of extracts from HeLa cells, untreated or treated with doxorubicin (0.5 μM, 24 hours) or with nocodazole (50 ng/ml, 24 hours), using Cyclin A (BF683) Mouse mAb #4656.
Western Blotting
Western blot analysis of extracts from A431 and H441 cells, untreated or EGF-treated, using Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb #4910 (upper) or Wee1 Antibody #4936 (lower).
Western Blotting
Western blot analysis of extracts from Saos-2 cells, untreated or treated with hydroxyurea or nocodazole, using Phospho-cdc2 (Tyr15) Antibody #9111 (upper) or cdc2 Antibody #9112 (lower).
| バックグラウンド |
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The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Tyr15 and Thr14 (1). Phosphorylation of cdc2 by the protein kinases Wee1 and Myt1 at Thr14 and Tyr15 results in inhibition of cdc2 (2,3). Progression through the G1/S checkpoint and initiation of DNA replication requires cyclin E; traversing the G2/M checkpoint to initiate mitosis requires cyclin B, and cyclin A may be required for both S-phase and M-phase (4). The versatile p21 cyclin-dependent kinase inhibitor, which interacts with several cyclin-CDK complexes to regulate cyclin-CDK during the cell cycle, is regulated by phosphorylation and ubiquitin-mediated degradation (5). Phosphorylation of histone H3 at Ser10 and neighboring residues correlates with chromosomal condensation, which is essential for segregation of chromosomes during mitosis (6).
- Norbury, C. et al. (1991) EMBO J 10, 3321-9.
- McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
- Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.
- Pagano, M. et al. (1992) EMBO J 11, 961-71.
- Abbas, T. and Dutta, A. (2009) Nat Rev Cancer 9, 400-14.
- Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
| 使用例 | |
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本製品は試験研究用です。