MAP Kinase Signaling
Phospho-Erk1/2 Pathway Sampler Kit |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
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| #9911S | 1 Kit | 80,000  | ログインすると国内在庫状況がご確認いただけます。
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9911 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 4370:
- Flow IHC / Paraffin Immunofluorescence Immunoprecipitation Western Blotting
- 9154:
- Immunoprecipitation Western Blotting
- 9335:
- Western Blotting
- 9427:
- Western Blotting
- 9595:
- IHC / Paraffin Immunoprecipitation Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| Phospho-c-Raf (Ser338) (56A6) Rabbit mAb #9427 | 40 µl | W | H M R Mk | 74 | Rabbit IgG |
| Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154 | 40 µl | W IP | H M R Mk Dm (C) | 45 | Rabbit IgG |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 | 40 µl | W IP IHC-P IF-IC F | H M R Hm Mk Mi Dm Z B Dg Pg Sc (Ce) | 44, 42 | Rabbit IgG |
| Phospho-p90RSK (Ser380) (9D9) Rabbit mAb #9335 | 40 µl | W | H M R Mk (C) | 90 | Rabbit IgG |
| Phospho-MSK1 (Thr581) Antibody #9595 | 40 µl | W IP IHC-P | H M | 90 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| U0126 (MEK1/2 Inhibitor) #9903 | 0.3 milligrams |
| 貯法 | |
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| -20℃ |
| MSDSはこちらから |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from 293, NIH/3T3 and C6 cells, treated with λ phosphatase or TPA as indicated, using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370 (upper), or p44/42 MAP Kinase (137F5) Rabbit mAb #4695 (lower).
Western Blotting
Western blot analysis of extracts from untreated or TPA-treated 293 and NIH/3T3 cells using Phospho-p90RSK (Ser380) (9D9) Rabbit mAb #9335.
Western Blotting
Western blot analysis of extracts from NIH/3T3, HeLa and COS cells, untreated or treated with TPA, using Phospho-c-Raf (Ser338) (56A6) Rabbit mAb #9427.
Western Blotting
Western blot analysis of extracts from 293 cells untreated or treated with UV and HeLa cells untreated or treated with calyculin A using Phospho-MSK1 (Thr581) Antibody #9595.
Western Blotting
Western blot analysis of extracts from untreated or TPA-treated HeLa and NIH/3T3 cells, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154 (upper), or MEK1/2 Antibody #9122 (lower).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370.
IF-IC
Confocal immunofluorescent analysis of C2C12 cells, U0126-treated (left, #9903 10 μM for 1 h) or TPA-treated (right, #9905 200 nM for 15 min), using Phospho-p44/42 MAPK (Thr202/Tyr204) (D13.14.4E) Rabbit mAb #4370 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
| バックグラウンド |
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Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
- Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
- Baccarini, M. (2005) FEBS Lett 579, 3271-7.
- Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
- Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Marais, R. et al. (1993) Cell 73, 381-93.
- Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
- Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
| 使用例 | |
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本製品は試験研究用です。