Jak/Stat Pathway
Phospho-Stat Antibody Sampler Kit |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #9914S | 1 Kit | 91,000 | ログインすると国内在庫状況がご確認いただけます。
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9914 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 4441:
- Western Blotting
- 9134:
- ChIP Agarose ChIP Magnetic Immunofluorescence* Immunoprecipitation Western Blotting
- 9145:
- ChIP Agarose ChIP Magnetic Flow IHC / Frozen IHC / Paraffin Immunofluorescence* Immunoprecipitation Western Blotting
- 9171:
- ChIP Agarose ChIP Magnetic Immunoprecipitation Western Blotting
- 9359:
- Flow IHC / Paraffin Immunoprecipitation Western Blotting
- 9361:
- Flow Immunofluorescence* Immunoprecipitation Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| Phospho-Stat1 (Tyr701) Antibody #9171 | 40 µl | W IP ChIP | H M R (B) (Dg) | 84, 91 | Rabbit |
| Phospho-Stat2 (Tyr690) Antibody #4441 | 40 µl | W | H (R) (B) | 113 | Rabbit |
| Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 | 40 µl | W IP IHC-P IHC-F IF-IC F ChIP | H M R Mk (Hm) (B) (Pg) (Hr) | 79, 86 | Rabbit IgG |
| Phospho-Stat3 (Ser727) Antibody #9134 | 40 µl | W IP IF-IC ChIP | H M R (B) | 86 | Rabbit |
| Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 | 40 µl | W IP IHC-P F | H M (R) (Mk) (B) | 90 | Rabbit IgG |
| Phospho-Stat6 (Tyr641) Antibody #9361 | 40 µl | W IP IF-IC F | H (B) | 110 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
| 貯法 | |
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| -20℃ |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or IFN-a-treated (100 ng/ml,15 minutes), using Phospho-Stat2 (Tyr690) Antibody or total stat2 antibody.
Western Blotting
Western blot analysis of extracts from EGF treated (100 ng/ml) A431 cells, using Phospho-Stat3 (Ser727) Antibody #9134 and Stat3 Antibody #9132.
Western Blotting
Western blot analysis of extracts from IFN-a treated Jurkat cells and HeLa cells (left), as well as EGF treated A431 cells (right), using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145. Note that the basal phospho-Stat3 in A431 is detected by the antibody.
Western Blotting
Western blot analysis of extracts from IFN-alpha treated (100 ng/ml) and untreated HeLa cells, using Phospho-Stat1 (Tyr701) Antibody #9171 .
Western Blotting
Western blot analysis of extracts from K562 cells, untreated or treated with Gleevec, using Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 (top) and total Stat5 (3H7) Rabbit mAb #9358 (bottom).
Western Blotting
Western blot analysis of extracts from IL-4 treated (10 ng/ml) Daudi cells, using Phospho-Stat6 (Tyr641) Antibody #9361 or control Stat6 antibody.
| バックグラウンド |
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Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).
- Darnell Jr., J. et al. (1994) Science 264, 1415-1421.
- Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Caldenhoven, E. et al. (1996) J. Biol. Chem. 271, 13221-13227.
- Wen, Z. et al. (1995) Cell 82, 241-250.
- Yokogami, K. et al. (2000) Curr. Biol. 10, 47-50.
- Lim, C.P. and Cao, X. (1999) J. Biol. Chem. 274, 31055-31061.
- Bromberg, J. F. et al. (1999) Cell 98, 295-303.
- Su, L. et al. (1999) J. Biol. Chem. 274, 31770-31774.
- Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
- Cattaneo, E. et al. (1999) Trends Neurosci. 22, 365-369.
- Frank, D.A. (1999) Mol. Med. 5, 432-456.
| 使用文献 |
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- Lee, J. et al. (2011) Oncogene Applications: Western Blotting
本製品は試験研究用です。