Antibody Sampler Kits
Translational Control Antibody Sampler Kit |
イイネ！(1) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月9日11時30分 現在 |
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| #9918S | 1 Kit | 91,000 | ログインすると国内在庫状況がご確認いただけます。
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9918 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 2855:
- Flow IHC / Paraffin Immunofluorescence Western Blotting
- 3398:
- IHC / Paraffin Immunoprecipitation Western Blotting
- 4060:
- Flow IHC / Frozen* IHC / Paraffin Immunofluorescence Immunoprecipitation Western Blotting
- 4858:
- Flow IHC / Frozen IHC / Paraffin Immunofluorescence Western Blotting
- 9234:
- Western Blotting
- 9741:
- Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 | 40 µl | W IP IHC-P IHC-F IF-IC F | H M R Hm Mk Dm Z B (C) (X) (Dg) (Pg) | 60 | Rabbit IgG |
| Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 | 40 µl | W | H M R Mk | 70, 85 | Rabbit IgG |
| Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 | 40 µl | W IHC-P IHC-F IF-IC F | H M R Mk Sc (C) | 32 | Rabbit IgG |
| Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb #3398 | 40 µl | W IP IHC-P | H M R Mk Dm | 38 | Rabbit IgG |
| Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855 | 40 µl | W IHC-P IF-IC F | H M R Mk Dm | 15 to 20 | Rabbit IgG |
| Phospho-eIF4E (Ser209) Antibody #9741 | 40 µl | W | H M R Mk | 25 | Rabbit |
| Rapamycin (FRAP/mTOR Inhibitor) #9904 | 2 micrograms | 914.2 | |||
| LY294002 (PI3 Kinase Inhibitor) #9901 | 0.3 milligrams | ||||
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
| 貯法 | |
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| -20℃ |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from 293T cells using 4E-BP1 Antibody #9452 (upper) and Phospho-4E-BP1 (Thr37/46) Antibody #2855 (lower). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes.
Western Blotting
Western blot analysis of extracts from C2C12 cells, untreated or thapsigargin-treated, using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb #3398 (upper) or eIF2α Antibody #9722 (lower).
Western Blotting
Western blot analysis of extracts from PC-3 cells, untreated or LY294002/wortmannin-treated, and NIH/3T3 cells, serum-starved or PDGF-treated, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).
Western Blotting
Western blot analysis of extracts from PC12 and NIH/3T3 cells, treated with λ phosphatase, 20% FBS (20 min) or 100 ng/ml PDGF (20 min) as indicated, using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 (upper) or S6 Ribosomal Protein (5G10) Rabbit mAb #2217 (lower).
Western Blotting
Western blot analysis of extracts from serum starved or serum-treated (20%) 293, NIH/3T3, and PC12 cells, using Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 (upper), or p70 S6 Kinase (49D7) Rabbit mAb #2708 (lower).
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with serum, PD98059 or Dexamethasone, using Phospho-eIF4E (Ser209) Antibody #9741 (upper) or eIF4E Antibody #9742 (lower).
| バックグラウンド |
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Key steps in translational control occur at the level of eukaryotic initiation factor 4F (eIF4F) and p70 S6 kinase regulation. eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure. Several stimuli, such as insulin and various growth and survival factors, regulate the eIF4F complex and p70 S6 kinase primarily by triggering a signaling cascade dependent on sequential activation of PI3K, Akt/PKB and mTOR/FRAP kinases. Akt is activated by phosphorylation within the C-terminus at Ser473 and within the activation loop at Thr308 by phospholipid-dependent kinases. Inactivation in vivo of PI3K by the highly selective inhibitor LY294002 inhibits Akt and downstream elements of this cascade. Direct phosphorylation of mTOR/FRAP at Ser2448 by Akt is a key regulatory event controlling its kinase activity. mTOR/FRAP activity can be effectively blocked by Rapamycin, leading to inactivation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1), an inhibitor of translation initiation, and activation of p70 S6 kinases. Inactivation of 4E-BP1 by sequential phosphorylation causes the release of eIF4E, which, together with eIF4G and other factors, forms a functional eIF4F cap binding complex. p70 S6 kinases phosphorylates the 40S ribosomal subunit protein S6 and stimulates the translation of 5' oligopyrimidine tract containing mRNAs. The Erk pathway is also involved in regulation at this level by regulating the eIF4E kinase, Mnk1, and activating p70 S6 kinase. Tuberin, a product of the tumor supressor gene TSG2, is directly phosphorylated atThr1462 by Akt/PKB. Tuberin inhibits the mammalian target of rapamycin, mTOR, which results in inhibition of p70 S6 kinase and activation of 4E-BP1 and, therefore, inhibition of translation.
- Gingras, A. et al. (1999) Annu. Rev. Biochem. 68, 913-963.
- Gingras, A. C. et al. (2001) Genes and Develop. 15, 807-826.
- Dennis, P. B. et al. (1999) Curr. Opin. Genet. Dev. 9, 49-54.
- Volarevic, S. and Thomas, G. (2000) Prog. Nucleic Acid Res. Mol. Biol. 65, 101-127.
- Pyronnet, S. et al. (2000) Biochem. Pharmacol. 60, 1237-1243.
- Dever, T.E. (2002) Cell 108, 545-556.
- Goncharova, E. et al. (2002) J. Biol. Chem. 277, 30958-30967.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。