Nuclear Receptor Signaling
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| CSTコード | 包装 | 希望納入価格（円） |
国内在庫  2010年7月30日15時15分 現在 |
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| #9924 | 1 Kit | 61,000 | ログインすると国内在庫状況がご確認いただけます。
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推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 2511:
- IHC / Paraffin Western Blotting
- 2512:
- Western Blotting
- 2515:
- Western Blotting
- 2517:
- Immunofluorescence Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| Estrogen Receptor α (62A3) Mouse mAb # 2512 | 40 microliters | W | H | 66 | Mouse IgG2a |
| Phospho-Estrogen Receptor α (Ser118) Antibody # 2515 | 40 microliters | W | H | 66 | Rabbit |
| Phospho-Estrogen Receptor α (Ser104/106) Antibody # 2517 | 40 microliters | W IF-IC | H (M) | 66 | Rabbit |
| Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb # 2511 | 40 microliters | W IHC-P | H | 66 | Mouse IgG2b |
| Anti-rabbit IgG, HRP-linked Antibody # 7074 | 100 microliters | Goat | |||
| Anti-mouse IgG, HRP-linked Antibody # 7076 | 50 microliters | Horse |
| 貯法 | |
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| -20℃ | |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from MCF-7 cells, and COS-1 cells transfected with Wild-type and mutant ER α, stimulated with EGF and E2, using Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb #2511 (upper) and control Estrogen Receptor α Antibody #2512 (lower).
Western Blotting
Western blot analysis of extracts from 293 cells expressed Wild-type ER alpha and MCF-7 cells, using Estrogen Receptor alpha 62A3 Monoclonal Antibody #2512.
Western Blotting
Western blot analysis of MCF-7 cell extracts treated with or without EGF (100 ng/ml) and E2 (10-7M) for 30 minutes, using Phospho-Estrogen Receptor alpha (Ser118) Antibody #2515 (upper) and control ER alpha antibody (lower).
Western Blotting
Western blot analysis of COS-1 cell extracts expressed Wild-type ER alpha and mutants (S106A, S118A and S167A) treated with EGF (100 ng/ml) and E2 (10-7M) for 30 minutes, using Phospho-Estrogen Receptor alpha (Ser118) Antibody #2515 (upper) and control ER alpha antibody (lower).
Western Blotting
Dephosphorylation of Wild-type ER alpha abolished Phospho-Estrogen Receptor alpha (Ser104/106) Antibody's (#2517) recognition.
Western Blotting
Western blot analysis of extracts from COS-1 cells expressed Wild-type ER alpha and mutants and stimulated with beta-estradiol (100 nM) and EGF (100 ng/ml) for 30 minutes, using Phospho-Estrogen Receptor alpha (Ser104/106) Antibody #2517 (upper) and control ER alpha antibody (lower). (Cell lysates provided by Dr. Simak Ali, Hammersmith Hospital, London.)
IHC-P (paraffin)
Immunohistochemical staining of phosphorylated estrogen receptor alpha in paraffin-embedded human breast carcinoma showing nuclear localization, using Phospho-Estrogen Receptor alpha (Ser118) 16J4 Monoclonal Antibody #2511.
IHC-F (frozen)
Immunocytochemical staining of MCF-7 cells using Estrogen Receptor alpha 62A3 Monoclonal Antibody #2512 (left) or this antibody pretreated with ER alpha-specific peptides (right).
IF-IC
Immunofluorescent staining of MCF-7 cells unstimulated (left) or stimulated with beta-estradiol (100 nM) and EGF (100 ng/ml) for 30 minutes (right), using Phospho-Estrogen Receptor alpha (Ser104/106) Antibody #2517.
| バックグラウンド |
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Estrogen receptorα (ER α), a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD) (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER α regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation provides an important mechanism to regulate ER α activity (3,4). ER α is phosphorylated on multiple sites (5). Serines 104, 106, 118 and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ER α activity. Ser118 may be the substrate of the transcription regulatory kinase cdK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients (4).
- Mangelsdorf, D.J. et al. (1995) Cell 83, 835-839.
- Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev. 14, 121-141.
- Chen, D. et al. (1999) Mol. Cell. Biol. 19, 1002-1015.
- Campbell, R.A. et al. (2001) J. Biol. Chem. 276, 9817-9824.
- Chen, D. et al. (2000) Mol. Cell 6, 127-137.
- Joel, P.B. et al. (1998) Mol. Cell. Biol. 18, 1978-1984.
| 使用例 | |
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本製品は試験研究用です。
