Tyrosine Kinases / Adaptors
Src Antibody Sampler Kit |
イイネ！(1) | 
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日17時20分 現在 |
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| #9935S | 1 Kit | 80,000 | ログインすると国内在庫状況がご確認いただけます。
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9935 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 2102:
- Immunoprecipitation Western Blotting
- 2105:
- IHC / Paraffin Western Blotting
- 2107:
- Western Blotting
- 2123:
- Immunoprecipitation Western Blotting
- 6943:
- Immunoprecipitation Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
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| Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943 | 40 µl | W IP | H M (R) | 60 | Rabbit IgG |
| Non-phospho-Src (Tyr527) Antibody #2107 | 40 µl | W | H M R (C) | 60 | Rabbit |
| Non-phospho-Src (Tyr416) (7G9) Mouse mAb #2102 | 40 µl | W IP | H M R (C) (X) | 60 | Mouse IgG2b |
| Phospho-Src (Tyr527) Antibody #2105 | 40 µl | W IHC-P | H M R (C) | 60 | Rabbit |
| Src (32G6) Rabbit mAb #2123 | 40 µl | W IP | H M R (C) | 60 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat | |||
| Anti-mouse IgG, HRP-linked Antibody #7076 | 100 µl | Horse |
| 貯法 | |
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| -20℃ |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from pervanadate-treated (1 mM for 5 minutes) NIH/3T3 cells that have been stably transfected with a constitutively active form of Src (in which the regulatory tyrosine 527 residue has been mutated to phenylalanine), A431 cells and HepG2 cells, using Non-phospho-Src (Tyr416) (7G9 ) mAb #2102 (upper), v-Src antibody (middle) or Phospho-Src (Tyr416) Antibody #2101 (lower).
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, using Phospho-Src (Tyr527) Antibody #2105 (A, B) or v-Src antibody (C, D). The phospho-specificity of the antibody was determined by treating the membrane with (B, D) or without (A, C) calf intestinal alkaline phosphatase (CIP) after Western transfer.
Western Blotting
Western blot analysis of extracts from A431 cells, untreated or pervanidate-treated (1 mM, 15 minutes), using Non-phospho-Src (Tyr527) Antibody #2107 (upper) or v-Src antibody (lower).
Western Blotting
Western blot analysis of cell extracts from various cell lines, using Src (32G6) Rabbit mAb #2123.
Western Blotting
Western blot analysis of extracts NIH/3T3 cells, serum-starved or treated with human Platelet-Derived Growth Factor BB hPDGF-BB #8912 (100 ng/ml, 15 min), using Phospho-Src Family (Tyr416) (D49G8) Rabbit mAb #6943 (upper) or Src (36D10) Rabbit mAb #2109 (lower).
IHC-P (paraffin)
Immunohistochemical staining of paraffin-embedded human breast carcinoma, showing membrane localization of phosphorylated Src, using Phospho-Src (Tyr527) Antibody #2105.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Src (36D10) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
IF-IC
Immunofluorescent staining of MCF-7 cells, using Src (36D10) Rabbit mAb #2109 alone (A), or the same antibody after a 1 hour preincubation with antigenic peptide (B).
| バックグラウンド |
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The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
Lyn is a member of the Src family that is predominantly expressed in hematopoietic cells (3). Lyn participates in signaling from multiple cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (4).Lck is essential for T-lymphocyte activation and differentiation (5,6). The activity of Lck is regulated by protein kinases and phosphatases. Phosphorylation of the C-terminal tyrosine 505 serves to downregulate Lck catalytic activity, while phosphorylation at tyrosine 394 leads to an increase in Lck activity (7).
- Thomas, S.M. and Brugge, J.S. (1997) Annu. Rev. Cell Dev. Biol. 13, 513-609.
- Hunter, T. (1987) Cell 49, 1-4.
- Yamanashi, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
- Yamanashi, Y. et al. (1991) Science 251, 192-194.
- Molina, T. J. et al. (1992) Nature 357, 161-164.
- Straus, D. B. et al. (1992) Cell 70, 585-593.
- Chow, L. M. et al. (1993) Nature 365, 156-160.
| 使用例 | |
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本製品は試験研究用です。