Antibody Sampler Kits
Phospho-IKKα/β (Ser176/180) Antibody Sampler Kit |
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月8日11時35分 現在 |
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| #9958S | 1 Kit | 56,000 | ログインすると国内在庫状況がご確認いただけます。
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9958 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 2078:
- Western Blotting
- 2694:
- Western Blotting
- 2697:
- IHC / Frozen IHC / Paraffin Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb #2078 | 40 µl | W | H M (R) (Mk) (B) | 85 (IKKalpha), 87 (IKKbeta) | Rabbit IgG |
| Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697 | 40 µl | W IHC-P IHC-F | H M R Mk (B) | 85 IKK-alpha 87 IKK-beta | Rabbit IgG |
| Phospho-IKKα/β (Ser176/180) Antibody II #2694 | 40 µl | W | H M R Mk | 85 IKK-alpha 87 IKK-beta | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
| 貯法 | |
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| -20℃ |
| ※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。 |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from differentiated THP-1 cells, untreated or LPS-treated for 15 minutes, using Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb #2078 (upper), IKKα Antibody #2682 (middle) and IKKβ (2C8) Rabbit mAb #2370 (lower).
Western Blotting
Western blot analysis of extracts from HeLa cells, treated with TNF-α (20ng/ml) for the indicated times, using Phospho-IKKα/β (Ser176/180) Antibody II #2694.
Western Blotting
Western blot analysis of extracts from HeLa and NIH/3T3 cells, treated with TNF-α (20 ng/ml) and calyculin A #9902 (50 nM), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697.
| バックグラウンド |
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The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation of Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes resulting in kinase activation (10-13).
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- Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
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- Brown, K. et al. (1995) Science 267, 1485-8.
- Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Zandi, E. et al. (1997) Cell 91, 243-52.
- Karin, M. (1999) Oncogene 18, 6867-74.
- DiDonato, J.A. et al. (1997) Nature 388, 548-54.
- Mercurio, F. et al. (1997) Science 278, 860-6.
- Johnson, L.N. et al. (1996) Cell 85, 149-58.
- Delhase, M. et al. (1999) Science 284, 309-13.
| 使用例 | |
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| 製品をご使用いただいて研究を発表されましたら、ぜひお知らせください。 |
本製品は試験研究用です。