TGF-beta/Smad Signaling
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| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2012年2月7日15時25分 現在 |
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| #9963S | 1 Kit | 126,000 | ログインすると国内在庫状況がご確認いただけます。
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9963 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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- 3108:
- Western Blotting
- 5339:
- ChIP Magnetic Flow Immunofluorescence Immunoprecipitation Western Blotting
- 6944:
- ChIP Agarose ChIP Magnetic Flow Immunofluorescence Immunoprecipitation Western Blotting
- 9515:
- ChIP Agarose ChIP Magnetic Western Blotting
- 9516:
- Flow Immunofluorescence Western Blotting
- 9517:
- Western Blotting
- 9519:
- Western Blotting
- 9520:
- ChIP Agarose ChIP Magnetic Immunoprecipitation Western Blotting
- 9523:
- ChIP Agarose ChIP Magnetic Flow Immunofluorescence Immunoprecipitation Western Blotting
| キット内容 | 容量 | 用途 | 種交差性 | 検出タンパク質の分子量 | Isotype |
|---|---|---|---|---|---|
| Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 | 40 µl | W IF-IC F | H M R | 60 | Rabbit IgG |
| Smad1 (D59D7) XP® Rabbit mAb #6944 | 40 µl | W IP IF-IC F ChIP | H M (Mk) (X) (B) | 60 | Rabbit IgG |
| Smad5 Antibody #9517 | 40 µl | W | H R Mk | 60 | Rabbit |
| Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 | 40 µl | W | H M R Mi | 60 | Rabbit IgG |
| Smad2 (D43B4) XP® Rabbit mAb #5339 | 40 µl | W IP IF-IC F ChIP | H M R Mk | 60 | Rabbit IgG |
| Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520 | 40 µl | W IP ChIP | H M R (Mk) (X) (Z) (B) | 52 | Rabbit IgG |
| Smad3 (C67H9) Rabbit mAb #9523 | 40 µl | W IP IF-IC F ChIP | H M R Mk (X) (Z) (B) | 52 | Rabbit IgG |
| Smad4 Antibody #9515 | 40 µl | W ChIP | H M R Mk | 70 | Rabbit |
| Smad6 Antibody #9519 | 40 µl | W | H Mk | 62 | Rabbit |
| Anti-rabbit IgG, HRP-linked Antibody #7074 | 100 µl | Goat |
| 貯法 | |
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| -20℃ |
| 社内データ |
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Western Blotting
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TGF-β-treated , using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 (upper) or Smad2 Antibody #3102 (lower).
Western Blotting
Western blot analysis of extracts from various cell lines using Smad2 (D43B4) XP® Rabbit mAb #5339.
Western Blotting
Western blot analysis of extracts from various cell lines using Smad1 (D59D7) XP® Rabbit mAb #6944.
Western Blotting
Western blot analysis of extracts from various cell lines using Smad4 Antibody #9515.
Western Blotting
Western blot analysis of extracts from HeLa or NIH/3T3 cells,untreated or BMP-4-treated, using Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516.
Western Blotting
Western blot analysis of extracts from various cell lines using Smad5 Antibody #9517.
Western Blotting
Western blot analysis of extracts from A204, LN18 and L929 cells using Smad6 Antibody #9519.
Western Blotting
Western blot analysis of extracts from HT-1080 cells, untreated or treated with TGF-β1, TGFR inhibitor SB-431542 or BMP-2, using Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520 (top) or total Smad3 (C67H9) Rabbit mAb #9523 (bottom).
Western Blotting
Western blot analysis of extracts from HT1080 (human), C2C12 (mouse) and B35 (rat) using Smad3 (C67H9) Rabbit mAb #9523.
| バックグラウンド |
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Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).
- Heldin, C.H. et al. (1997) Nature 390, 465-471.
- Attisano, L. and Wrana, J.L. (1998) Curr. Opin. Cell Biol. 10, 188-194.
- Derynck, R. et al. (1998) Cell 95, 737-740.
- Massague, J. (1998) Annu. Rev. Biochem. 67, 753-791.
- Whitman, M. (1998) Genes Dev. 12, 2445-2462.
- Wu, G. et al. (2000) Science 287, 92-97.
- Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-1647.
- Moustakas, A. et al. (2001) J. Cell Sci. 114, 4359-4369.
| 使用文献 |
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- Yao, Z. et al. (2010) Proc Natl Acad Sci U S A 107, 15535-40. Applications: Western Blotting
本製品は試験研究用です。