| Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855 | HeLa | Untreated | |
| Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) #3929 | HeLa | Untreated | |
| Phospho-4E-BP1 (Thr37/46) Antibody #9459 | HeLa | Untreated | |
| Phospho-4E-BP1 (Ser65) (174A9) Rabbit mAb #9456 | HeLa | Untreated | |
| Phospho-4E-BP1 (Ser65) Antibody #9451 | HeLa | Untreated | |
| Phospho-4E-BP1 (Thr70) Antibody #9455 | HeLa | Untreated | |
| Phospho-53BP1 (Ser25/29) Antibody #2674 | HT-29 | UV‡ (100 mJ/cm2), 1 hr recovery before lysing | |
| Phospho-53BP1 (Thr543) Antibody #3428 | HeLa | UV‡ (100 mJ/cm2), 1 hr recovery | |
| Phospho-53BP1 (Ser1778) Antibody #2675 | HeLa | UV‡ (100 mJ/cm2), 1 hr recovery before lysing | |
| Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb #3098 | K-562 | Untreated (K-562 cell line contains Bcr-Abl fusion) | |
| Phospho-c-Abl (Tyr204) (C42B5) Rabbit mAb #3009 | K-562 | Untreated (K-562 cell line contains Bcr-Abl fusion) | |
| Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb #2868 | K-562 | Untreated (K-562 cell line contains Bcr-Abl fusion) | |
| Phospho-c-Abl (Tyr245) Antibody #2861 | K-562 | Untreated (K-562 cell line contains Bcr-Abl fusion) | |
| Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb #2865 | K-562 | Untreated (K-562 cell line contains Bcr-Abl fusion) | |
| Phospho-c-Abl (Thr735) Antibody #2864 | K-562 | Untreated (K-562 cell line contains Bcr-Abl fusion) | |
| Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 | C2C12 | Untreated | AMPK Control Cell Extracts #9158 |
| Acetyl-CoA Carboxylase 1 Antibody #4190 | C2C12 | Untreated | AMPK Control Cell Extracts #9158 |
| Acetyl-CoA Carboxylase Antibody #3662 | C2C12 | Untreated | AMPK Control Cell Extracts #9158 |
| Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Acetylated-Lysine (Ac-K2-100) Rabbit mAb #9814 | COS | TSA (0.4 μM for 18 hrs) | |
| Acetylated-Lysine (Ac-K2-100) Rabbit mAb (HRP Conjugate) #6952 | COS | TSA (0.4 uM for 18 hrs) | |
| Acetylated-Lysine Mouse mAb (Ac-K-103) #9681 | COS | TSA (0.4 μM for 18 hrs) | |
| Acetylated-Lysine Antibody #9441 | COS | TSA (0.4 μM for 18 hrs) | |
| Phospho-Ack1 (Tyr284) Antibody #3138 | Transfected† | Transfect cells with construct overexpressing Ack1 | |
| Phospho-Ack1 (Tyr857/858) (C57E10) Rabbit mAb #3097 | Transfected† | Transfect cells with construct overexpressing Ack1 | |
| Phospho-Ack1 (Tyr857/858) Antibody #3137 | Transfected† | Transfect cells with construct overexpressing Ack1 | |
| Phospho-Afadin (Ser1718) Antibody #5485 | A-431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | |
| Phospho-Akt Substrate (RXRXXS/T) (110B7E) Rabbit mAb #9614 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (HRP Conjugate) #6950 | Jurkat | Calyculin A (100 nM for 30 min) | |
| Phospho-(Ser/Thr) Akt Substrate Antibody #9611 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Thr308) (244F9) Rabbit mAb #4056 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Thr308) (L32A4) Mouse mAb #5106 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Thr308) Antibody #9275 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Thr450) Antibody #9267 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 | Jurkat | Untreated or Calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100 nM for 10 min); works for most cell lines | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Biotinylated) #5012 | Jurkat | Untreated or Calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100 nM for 10 min); works for most cell lines | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (HRP Conjugate) #5171 | C2C12 | Serum-starve overnight, insulin (100 nM 10 min) | |
| Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (PE Conjugate) #5315 | C2C12 | Serum-starve overnight, insulin (100 nM 10 min) | |
| Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058 | Jurkat | Untreated or Calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100 nM for 10 min); works for most cell lines | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Ser473) (736E11) Rabbit mAb #3787 | LNCaP | Untreated | SignalSlide® Phospho-Akt (Ser473) IHC Controls #8101 |
| Phospho-Akt (Ser473) (587F11) Mouse mAb #4051 | Jurkat | Untreated or Calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100 nM for 10 min); works for most cell lines | Akt Control Cell Extracts #9273 |
| Phospho-Akt (Ser473) Antibody #9271 | Jurkat | Untreated or Calyculin A (100 nM for 30 min); alternative: serum-starve, insulin (100 nM for 10 min); works for most cell lines | Akt Control Cell Extracts #9273 |
| Phospho-Drosophila Akt (Ser505) Antibody #4054 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-ALK (Tyr1078) Antibody #4144 | KARPAS-299 | Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) | |
| Phospho-ALK (Tyr1096) (D96H9) Rabbit mAb #6962 | KARPAS-299 | Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) | |
| Phospho-ALK (Tyr1096) Antibody #4143 | KARPAS-299 | Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) | |
| Phospho-ALK (Tyr1278) (D59G10) Rabbit mAb #6941 | KARPAS-299 | Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) | |
| Phospho-ALK (Tyr1278) Antibody #3710 | KARPAS-299 | Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) | |
| Phospho-ALK (Tyr1278/1282/1283) Antibody #3983 | KARPAS-299 | Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) | |
| Phospho-ALK (Tyr1282/1283) Antibody #3714 | KARPAS-299 | Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) | |
| Phospho-ALK (Tyr1586) (3B4) Rabbit mAb #3348 | KARPAS-299 | Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) | |
| Phospho-ALK (Tyr1586) Antibody #3343 | KARPAS-299 | Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) | |
| Phospho-ALK (Tyr1604) Antibody #3341 | KARPAS-299 | Untreated (KARPAS-299 cell lines contains NPM-ALK fusion) | |
| Cleaved α-Fodrin (Asp1185) Antibody #2121 | HeLa | Serum-starve overnight, Staurosporine (1.0 μM for 4 hrs) | |
| Phospho-AML1 (Ser249) Antibody #4327 | Jurkat, HEL | Untreated | |
| Phospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb #5759 | C2C12 | Serum-starve 30 min | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb #4188 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKα (Thr172) Antibody #2531 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKα1 (Ser485)/AMPKα2 (Ser491) Antibody #4185 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKα1 (Ser485) (45F5) Rabbit mAb #2537 | 293 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKα1 (Ser485) Antibody #4184 | C6 | Serum-starve overnight, Oligomycin (0.5 μM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKβ1 (Ser108) Antibody #4181 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AMPKβ1 (Ser182) Antibody #4186 | C2C12 | Serum-starve overnight, AICAR (0.5 mM for 30 min) | AMPK Control Cell Extracts #9158 |
| Phospho-AP2M1 (Thr156) Antibody #3843 | HeLa | H2O2 (4 mM for 30 min) | |
| Phospho-APP (Thr668) (D90B8) Rabbit mAb #6986 | HeLa | Nocodazole (1 µg/ml for 18 hrs) | |
| Phospho-APP (Thr668) Antibody #3823 | HeLa | Nocodazole (1 µg/ml for 18 hrs) | |
| Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb #5617 | HeLa | Untreated | |
| Phospho-β-Arrestin 1 (Ser412) (6-24) Mouse mAb #2416 | 293 | Untreated | |
| Phospho-AS160 (Ser318) (D3D11) Rabbit mAb #8619 | HeLa | Serum-starve overnight, hIGF-1 (100 ng/ml for 15 min) | |
| Phospho-AS160 (Ser588) (D8E4) Rabbit mAb #8730 | HeLa | Serum-starve overnight, hIGF-1 (100 ng/ml for 15 min) | |
| Phospho-AS160 (Thr642) Antibody #4288 | HeLa | Serum-starve overnight, hIGF-I (100 ng/ml for 15 min) | |
| Phospho-ASK1 (Ser83) Antibody #3761 | Transfected† | Transfect with construct overexpressing ASK1 | |
| Phospho-ASK1 (Thr845) Antibody #3765 | Transfected† | Transfect with construct overexpressing ASK1 | |
| Phospho-ASK1 (Ser967) Antibody #3764 | Transfected† | Transfect with construct overexpressing ASK1 | |
| Phospho-ATF-2 (Thr69/71) Antibody #9225 | NIH/3T3 | Anisomycin (25 μg/ml for 30 min) | ATF-2 Control Cell Extracts #9223 |
| Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb #5112 | NIH/3T3 | Anisomycin (25 μg/ml for 30 min) | ATF-2 Control Cell Extracts #9223 |
| Phospho-ATF-2 (Thr71) (11G2) Rabbit mAb (Biotinylated) #4036 | NIH/3T3 | Anisomycin (25 μg/ml for 30 min) | ATF-2 Control Cell Extracts #9223 |
| Phospho-ATF-2 (Thr71) Antibody #9221 | NIH/3T3 | Anisomycin (25 μg/ml for 30 min) | ATF-2 Control Cell Extracts #9223 |
| Phospho-ATM (Ser1981) (10H11.E12) Mouse mAb #4526 | 293 | IR (10 Gy), 1 hr recovery | |
| Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb #2909 | 293 | UV‡ (50 mJ/cm2), 30 min recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb #9607 | 293 | UV‡ (50 mJ/cm2), 30 min recovery | |
| Phospho-(Ser/Thr) ATM/ATR Substrate Antibody #2851 | 293 | UV‡ (50 mJ/cm2), 30 min recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb #6966 | 293 | UV‡ (50 mJ/cm2), 30 min recovery | |
| Phospho-ATP-Citrate Lyase (Ser454) Antibody #4331 | NIH/3T3 | Serum-starve overnight, PDGF (50 ng/ml for 10 min) | |
| Phospho-Na,K-ATPase α1 (Tyr10) Antibody #3060 | A-431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | |
| Phospho-Na,K-ATPase α1 (Ser16) Antibody #4020 | PC-12 | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-Na,K-ATPase α1 (Ser23) Antibody #4006 | PC-12 | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-ATR (Ser428) Antibody #2853 | HeLa | UV‡ (75 mJ/cm2), 1 hr recovery | |
| Phospho-ATRIP (Ser224) Antibody #5161 | HeLa | Untreated | |
| Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAb #2914 | HT-29 | Nocodazole (100 ng/ml for 18 hrs) - treatment induces signal | |
| Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb #3079 | HT-29 | Nocodazole (100 ng/ml for 18 hrs) - treatment induces signal | |
| Phospho-Axl (Tyr702) (D12B2) Rabbit mAb #5724 | NCI-H1299 | Gas6 (400 ng/mL for 5 min) | |
| Phospho-Bad (Ser112) (40A9) Rabbit mAb #5284 | COS | Serum-starve overnight, TPA (200 nM for 30 min) | Bad Control Proteins #9293 |
| Phospho-Bad (Ser112) (7E11) Mouse mAb #9296 | COS | Serum-starve overnight, TPA (200 nM for 30 min) | Bad Control Proteins #9293 |
| Phospho-Bad (Ser112) Antibody #9291 | COS | Serum-starve overnight, TPA (200 nM for 30 min) | Bad Control Proteins #9293 |
| Phospho-Bad (Ser136) (185D10) Rabbit mAb #5286 | COS | Serum-starve overnight, TPA (200 nM for 30 min) | Bad Control Proteins #9293 |
| Phospho-Bad (Ser136) (D25H8) Rabbit mAb #4366 | ACHN | Serum-starve overnight, hEGF (100 ng/ml for 30 min) | Bad Control Proteins #9293 |
| Phospho-Bad (Ser136) Antibody #9295 | Transfected† | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | Bad Control Proteins #9293 |
| Phospho-Bad (Ser155) Antibody #9297 | Transfected† | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | Bad Control Proteins #9293 |
| Phospho-BAP1 (Ser592) Antibody #9373 | HeLa | Serum-starve overnight, UV‡ (50 mJ/cm2), 1 hr recovery | |
| Phospho-Bcl-2 (Thr56) Antibody (Human Specific) #2875 | RL-7 | Paclitaxel (50 nM for 20 hrs) | |
| Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb #2827 | Jurkat | Paclitaxel (1 μM for 20 hrs) | |
| Phospho-Bcr (Tyr177) Antibody #3901 | K-562 | Untreated (K-562 cell line contains Bcr-Abl fusion) | |
| Phospho-Bim (Ser55) Antibody #4550 | A20 | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-Bim (Ser69) (D7E11) Rabbit mAb #4585 | Raji, A20 | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-Bim (Ser69) Antibody #4581 | Raji, A20 | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-BLNK (Tyr96) Antibody #3601 | Ramos | Anti-IgM (12 µg/ml for 2 min) | |
| Phospho-BRCA1 (Ser1524) Antibody #9009 | HeLa | UV‡ (75 mJ/cm2), 1 hr recovery | |
| Phospho-Btk (Ser180) (3D3) Mouse mAb #3537 | THP-1 | Serum-starve overnight, H2O2 (2 mM for 2 min) | |
| Phospho-Btk (Tyr223) Antibody #5082 | Ramos | Anti-IgM (12 μg/ml for 10 min) | |
| Phospho-C/EBPα (Ser21) Antibody #2841 | differentiated 3T3-L1 | Serum-starve overnight, insulin (100 nM for 45 min) | |
| Phospho-C/EBPα (Thr222/226) Antibody #2844 | U-937 | Untreated | |
| Phospho-C/EBPβ (Ser105) Antibody (Rat Specific) #3081 | PC-12 | Untreated | |
| Phospho-C/EBPβ (Thr235) Antibody #3084 | differentiated 3T3-L1 | Serum-starve overnight, insulin (100 nM for 10 min) | |
| Phospho-CaMKII (Tyr231) Antibody #3356 | Rat brain, mouse brain, human brain, SHSY5Y, Neuro2A | | |
| Phospho-CaMKII (Thr286) Antibody #3361 | HeLa | TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) #9509 | L-929 | Serum-starve overnight, Staurosporine (1 µM for 3 hrs) | |
| Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664 | HeLa | Serum-starve overnight, Staurosporine (1 μM for 3 hrs) | Caspase-3 Control Cell Extracts #9663 |
| Cleaved Caspase-3 (Asp175) Antibody #9661 | HeLa | Serum-starve overnight, Staurosporine (1 μM for 3 hrs) | Caspase-3 Control Cell Extracts #9663 |
| Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199 | THP-1 | TPA (80 nM overnight) plus LPS (1 µg/ml for 8 hrs) | |
| Cleaved Caspase-6 (Asp162) Antibody #9761 | HeLa | Serum-starve overnight, Staurosporine (1 μM for 3 hrs) | Caspase-3 Control Cell Extracts #9663 |
| Cleaved Caspase-7 (Asp198) Antibody #9491 | HeLa | Serum-starve overnight, Staurosporine (1 μM for 3 hrs) | Caspase-3 Control Cell Extracts #9663 |
| Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496 | Jurkat | Etoposide (25 μM for 5 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
| Cleaved Caspase-8 (Asp384) (11G10) Mouse mAb #9748 | Jurkat | Etoposide (25 μM for 5 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
| Cleaved Caspase-8 (Asp387) (D5B2) XP® Rabbit mAb (Mouse Specific) #8592 | CTLL-2 | Cycloheximide (10 µg/ml overnight) plus mTNF-α (20 ng/ml for 4 hrs) | |
| Cleaved Caspase-8 (Asp387) Antibody (Rodent Specific) #9429 | CTLL-2 | Cycloheximide (10 µg/ml overnight) plus mTNF-α (20 ng/ml for 4 hrs) | |
| Cleaved Caspase-9 (Asp353) Antibody (Rat Specific) #9507 | C6 | Serum-starve overnight, Staurosporine (1 µM for 3 hrs) | |
| Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb #7237 | HeLa | Serum-starve overnight, Staurosporine (1 µM for 3 hrs) | |
| Cleaved Caspase-9 (Asp330) Antibody (Human Specific) #9501 | Jurkat | Etoposide (25 μM for 5 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
| Cleaved Caspase-9 (Asp315) Antibody (Human Specific) #9505 | Jurkat | Etoposide (25 μM for 5 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
| Phospho-β-Catenin (Ser33/37) Antibody #2009 | SW480 | Untreated | |
| Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561 | SW480 | Untreated | |
| Phospho-β-Catenin (Ser45) Antibody #9564 | SW480 | Untreated | |
| Phospho-β-Catenin (Thr41/Ser45) Antibody #9565 | SW480 | Untreated | |
| Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb #5651 | SK-N-MC | Forskolin (50 µM for 30 min) | |
| Phospho-β-Catenin (Ser552) Antibody #9566 | SK-N-MC | Forskolin (50 μM for 30 min) | |
| Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb #4176 | SK-N-MC | Forskolin (50 μM for 30 min) | |
| Phospho-β-Catenin (Ser675) Antibody #9567 | SK-N-MC | Forskolin (50 μM for 30 min) | |
| Acetyl-β-Catenin (Lys49) Antibody #9534 | Transfected† | Transfect with a construct overexpressing CBP | |
| Phospho-Catenin δ-1 (Tyr228) Antibody #2911 | A-431 | Serum-starve overnight, hEGF (100 ng/ml for 15 min) | |
| Phospho-Catenin δ-1 (Ser252) Antibody #8477 | A-431 | Untreated | |
| Phospho-Catenin δ-1 (Ser320) Antibody #8016 | A-431 | Untreated | |
| Phospho-Catenin δ-1 (Tyr904) Antibody #2910 | A-431 | Serum-starve overnight, hEGF (100 ng/ml for 15 min) | |
| Phospho-Caveolin-1 (Tyr14) Antibody #3251 | HeLa | Serum-starve overnight, H2O2 (500 μM for 15 min) | |
| Phospho-c-Cbl (Tyr731) Antibody #3554 | K-562 | Untreated | |
| Phospho-c-Cbl (Tyr774) Antibody #3555 | K-562 | Untreated | |
| Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody #4771 | NIH/3T3 | TSA (400 nM overnight) | |
| Phospho-CD19 (Tyr531) Antibody #3571 | Ramos | Anti-IgM (12 μg/ml for 2 min) | |
| Phospho-cdc2 (Thr14) Antibody #2543 | HeLa | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-cdc2 (Thr161) Antibody #9114 | HeLa | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-cdc2 (Tyr15) (10A11) Rabbit mAb #4539 | HeLa | Untreated | cdc2 (Tyr15) Control Proteins #9113 |
| Phospho-cdc2 (Tyr15) Antibody #9111 | HeLa | Untreated | cdc2 (Tyr15) Control Proteins #9113 |
| Phospho-cdc25C (Thr48) Antibody #9527 | HT-29 | Nocodazole (100 ng/ml for 18 hrs) - treatment induces signal | |
| Phospho-cdc25C (Ser198) Antibody #9529 | Transfected† | Transfect with construct overexpressing cdc25C; Nocodazole (100 ng/ml for 18 hrs) - treatment induces signal | |
| Phospho-cdc25C (Ser216) (63F9) Rabbit mAb #4901 | HT-29 | Nocodazole (100 ng/ml for 18 hrs) - treatment induces signal | |
| Phospho-cdc25C (Ser216) Antibody #9528 | HT-29 | Nocodazole (100 ng/ml for 18 hrs) - treatment diminishes signal | |
| Phospho-CDC37 (Ser13) Antibody #8733 | 293 | Untreated | |
| Phospho-CDK2 (Thr160) Antibody #2561 | HeLa | Hydroxyurea (10 mM for 16 hrs) | |
| Phospho-CDK9 (Thr186) Antibody #2549 | HeLa | Untreated; site is constitutively phosphorylated | |
| Phospho-Chk1 (Ser280) Antibody #2347 | HeLa | UV‡ (75 mJ/cm2), 1 hr recovery | |
| Phospho-Chk1 (Ser296) Antibody #2349 | 293 | UV‡ (100 mJ/cm2), 1 hr recovery | |
| Phospho-Chk1 (Ser317) Antibody #2344 | HeLa | UV‡ (100 mJ/cm2), 1 hr recovery | |
| Phospho-Chk1 (Ser345) (133D3) Rabbit mAb #2348 | HeLa | UV‡ (75 mJ/cm2), 1 hr recovery | |
| Phospho-Chk1 (Ser345) Antibody #2341 | HeLa | UV‡ (100 mJ/cm2), 1 hr recovery | |
| Phospho-Chk2 (Ser19) Antibody #2666 | HeLa | UV‡ (100 mJ/cm2), 1 hr recovery | |
| Phospho-Chk2 (Ser33/35) Antibody #2665 | HeLa | UV‡ (75 mJ/cm2), 1 hr recovery | |
| Phospho-Chk2 (Thr68) Antibody #2661 | HeLa | IR (10 Gy), 30 min recovery | |
| Phospho-Chk2 (Ser516) Antibody #2669 | HeLa | UV‡ (100 mJ/cm2), 1 hr recovery | |
| Phospho-Cofilin (Ser3) (77G2) Rabbit mAb #3313 | C2C12, NIH/3T3, HeLa | Untreated | |
| Phospho-Cofilin (Ser3) Antibody #3311 | C2C12, NIH/3T3, HeLa | Untreated | |
| Phospho-Connexin 43 (Ser368) Antibody #3511 | COS | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-Cortactin (Tyr421) Antibody #4569 | HeLa | Serum-starve overnight, H2O2 (2 nM for 2 min) | |
| Phospho-CREB (Ser133) (87G3) Rabbit mAb #9198 | SK-N-MC | Forskolin (30 µM) and IBMX (0.5 mM), for 30 min | CREB Control Cell Extracts #9193 |
| Phospho-CREB (Ser133) (87G3) Rabbit mAb (Biotinylated) #4095 | SK-N-MC | Forskolin (30 µM) and IBMX (0.5 mM), for 30 min | CREB Control Cell Extracts #9193 |
| Phospho-CREB (Ser133) (D1G6) XP® Rabbit mAb #4276 | SK-N-MC | Forskolin (30 µM) and IBMX (0.5 mM), for 30 min | CREB Control Cell Extracts #9193 |
| Phospho-CREB (Ser133) (1B6) Mouse mAb #9196 | SK-N-MC | Forskolin (30 µM) and IBMX (0.5 mM), for 30 min | CREB Control Cell Extracts #9193 |
| Phospho-CREB (Ser133) Antibody #9191 | SK-N-MC | Forskolin (30 µM) and IBMX (0.5 mM), for 30 min | CREB Control Cell Extracts #9193 |
| Phospho-CrkII (Tyr221) Antibody #3491 | K-562 | Untreated | |
| Phospho-CrkL (Tyr207) Antibody #3181 | K-562 | Untreated | |
| Phospho-CRMP-2 (Thr514) Antibody #9397 | Rat brain | Untreated | |
| Phospho-M-CSF Receptor (Tyr546) Antibody #3083 | Transfected† | Transfect cells with a construct overexpressing CSFR1 | |
| Phospho-M-CSF Receptor (Tyr699) Antibody #3399 | NKM-1 | Untreated | |
| Phospho-M-CSF Receptor (Tyr708) Antibody #3080 | Transfected† | Transfect cells with a construct overexpressing CSFR1 | |
| Phospho-M-CSF Receptor (Tyr723) (49C10) Rabbit mAb #3155 | NKM-1 | Untreated | |
| Phospho-M-CSF Receptor (Tyr723) Antibody #3151 | NKM-1 | Untreated | |
| Phospho-M-CSF Receptor (Tyr809) Antibody #3154 | NKM-1 | Untreated | |
| Phospho-M-CSF Receptor (Tyr923) Antibody #3406 | NKM-1 | Untreated | |
| Phospho-CTDSPL2 (Ser104) Antibody #5294 | Hela | Untreated | |
| Phospho-Cyclin B1 (Ser133) (9E3) Rabbit mAb #4133 | HeLa | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-Cyclin B1 (Ser147) Antibody #4131 | HeLa | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-Cyclin D1 (Thr286) (D29B3) XP® Rabbit mAb #3300 | HT-1080 | MG132 (10 µM for 4 hrs) | |
| Phospho-Cyclin D1 (Thr286) Antibody #2921 | HT-1080 | MG132 (10 µM for 4 hrs) | |
| Phospho-Cyclin E (Thr62) Antibody #4136 | MCF-7 | Hydroxyurea (10 mM for 8 hrs) | |
| Phospho-CYLD (Ser418) Antibody #4500 | 293 | hTNF-α (20 ng/ml for 20 min) | |
| Phospho-Dab1 (Tyr220) Antibody #3327 | Transfected† | Cotransfect with Dab1 and fyn expression contructs | |
| Phospho-Dab1 (Tyr232) Antibody #3325 | Transfected† | Cotransfect with Dab1 and fyn expression contructs | |
| Phospho-DARPP-32 (Thr34) (D29E8) Rabbit mAb #5393 | Rat brain, mouse brain, human brain, SHSY5Y, Neuro2A | Untreated | |
| Phospho-DARPP-32 (Thr75) Antibody #2301 | Rat brain, mouse brain, SHSY5Y, Neuro2A | PKA (12,500 U/100 μl for 1 hr) | |
| Phospho-DBC1 (Thr454) Antibody #4880 | 293 | UV‡ (100 mJ/cm2), 4 hrs recovery | |
| Phospho-Doublecortin (Ser334) Antibody #3453 | Rat brain, mouse brain | Untreated | |
| Phospho-Desmoplakin (Ser165/166) Antibody #5885 | A-431 | Untreated | |
| Cleaved DFF45 (Asp224) Antibody #9731 | Jurkat | Etoposide (25 µM for 5 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
| Phospho-p56Dok-2(Tyr351) Antibody #3911 | Transfected† | Transfect cells with a construct overexpressing p56Dok-2 | |
| Phospho-Doublecortin (Ser297) Antibody #4605 | Fetal rat brain | Untreated | |
| Phospho-DR6 (Ser562) Antibody #7616 | A-431 | Etoposide (25 µM for 5 hrs) | |
| Phospho-DRP1 (Ser637) (D3A4) Rabbit mAb #6319 | PC-12 | Forskolin (20 μM for 1 hr) | |
| Phospho-DRP1 (Ser637) Antibody #4867 | PC-12 | PKA (12,500 U/100 μl for 1 hr) | |
| Phospho-eEF2 (Thr56) Antibody #2331 | C6 | Forskolin (10 µM for 1 hr) | |
| Phospho-eEF2k (Ser366) Antibody #3691 | HeLa, NIH/3T3, C6, COS | Untreated | |
| Phospho-EGF Receptor (Thr669) Antibody #3056 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-EGF Receptor (Tyr845) (D63B4) Rabbit mAb #6963 | A-431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | |
| Phospho-EGF Receptor (Tyr845) Antibody #2231 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-EGF Receptor (Tyr998) (C24A5) Rabbit mAb #2641 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-EGF Receptor (Tyr992) Antibody #2235 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-EGF Receptor (Tyr1045) Antibody #2237 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-EGF Receptor (Ser1046/1047) Antibody #2238 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb #3777 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb #2236 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-EGF Receptor (Tyr1068) Antibody #2234 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-EGF Receptor (Tyr1086) Antibody #2220 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-EGF Receptor (Tyr1148) Antibody #4404 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407 | A431 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | EGF Receptor Control Cell Extracts #5634 |
| Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb #3398 | HeLa | Thapsigargin (300 nM for 30 min) | |
| Phospho-eIF2α (Ser51) (119A11) Rabbit mAb #3597 | HeLa | Thapsigargin (300 nM for 30 min) | |
| Phospho-eIF2α (Ser51) Antibody #9721 | HeLa | Thapsigargin (300 nM for 30 min) | |
| Phospho-eIF4B (Ser406) Antibody #5399 | NIH/3T3 | Untreated | |
| Phospho-eIF4B (Ser422) Antibody #3591 | NIH/3T3 | Serum-starve overnight, 20% calf serum for 20 min | |
| Phospho-eIF4E (Ser209) Antibody #9741 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-eIF4G (Ser1108) Antibody #2441 | HeLa | Serum-starve overnight, insulin (100 nM for 5–10 min) | |
| Phospho-Elk-1 (Ser383) (2B1) Mouse mAb #9186 | recombinant protein | GST-Elk1 fusion protein phosphorylated in vitro by Erk1/2 | Elk-1 Control Proteins #9183 |
| Phospho-Elk-1 (Ser383) Antibody #9181 | recombinant protein | GST-Elk1 fusion protein phosphorylated in vitro by Erk1/2 | Elk-1 Control Proteins #9183 |
| Phospho-eNOS (Ser113) Antibody #9575 | BAEC | Untreated | |
| Phospho-eNOS (Thr495) Antibody #9574 | BAEC | Untreated, 3 days post-confluence | |
| Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb #9570 | BAEC | VEGF (50 ng/ml for 5 min) | |
| Phospho-eNOS (Ser1177) Antibody #9571 | BAEC | VEGF (50 ng/ml for 5 min) | |
| Phospho-EphA2 (Tyr588) Antibody #7423 | SNB19 | EphrinA1-Fc ligand (100 ng/ml for 5 min) | |
| Phospho-EphA2 (Tyr594) Antibody #3970 | Transfected† | Transfect cells with construct overexpressing EphA2 | |
| Phospho-EphA2 (Tyr772) Antibody #8244 | SNB19 | EphrinA1-Fc ligand (100 ng/ml for 5 min) | |
| Phospho-Ephrin B (Tyr324/329) Antibody #3481 | Transfected† | NIH/3T3 cells stably expressing Ephrin B1, stimulated with its cognate receptor EphB2-Fc | |
| Phospho-Erk5 (Thr218/Tyr220) Antibody #3371 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/ml for 5 min); IP with #3372 prior to Western | |
| Phospho-Estrogen Receptor α (Ser104/106) Antibody #2517 | MCF-7 | Serum-starve overnight, hEGF (100 ng/ml) plus estradiol (100 nM) for 30 min | |
| Phospho-Estrogen Receptor α (Ser118) (16J4) Mouse mAb #2511 | MCF-7 | Serum-starve overnight, hEGF (100 ng/ml) plus estradiol (100 nM) for 30 min | |
| Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb #5587 | MCF7 | Treated with Estrodiol/hEGF (100 nM each, together for 30 min) | |
| Phospho-Etk (Tyr40) Antibody #3211 | Jurkat | Serum-starve overnight, anti-CD3 (1 μg/ml) plus anti-CD28 (0.5 μg/ml) for 10 min | |
| Phospho-Ezrin (Tyr353) (D66D3) Rabbit mAb #5484 | A-431 | Serum-starve, hEGF (100 ng/ml for 2 min) | |
| Phospho-Ezrin (Tyr353) Antibody #3144 | A-431 | Serum-starve overnight, hEGF (100 ng/ml for 10 min) | |
| Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) (41A3) Rabbit mAb #3149 | C2C12, NIH/3T3, HeLa | Untreated | |
| Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody #3141 | C2C12, NIH/3T3, HeLa | Untreated | |
| Phospho-FADD (Ser191) Antibody (Mouse Specific) #2785 | A20 | Inhibit with hydroxyurea (4.0 mM for 24 hrs); activate with Nocodazole (1.0 μg/ml) for 24 hrs | |
| Phospho-FADD (Ser194) Antibody (Human Specific) #2781 | Jurkat | Inhibit with hydroxyurea (4.0 mM for 24 hrs); activate with Nocodazole (1.0 μg/ml) for 24 hrs | |
| Phospho-FAK (Tyr397) (D20B1) Rabbit mAb #8556 | A549 | Untreated | |
| Phospho-FAK (Tyr397) Antibody #3283 | A549 | Untreated | |
| Phospho-FAK (Tyr576/577) Antibody #3281 | A549 | Untreated | |
| Phospho-FAK (Tyr925) Antibody #3284 | A549 | Untreated | |
| Phospho-FANCD2 (Ser222) Antibody #4945 | HeLa | UV‡ (100 mJ/cm2), 1 hr recovery | |
| Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb #3476 | Transfected† | Transfect with a construct overexpressing FGFR1 | |
| Phospho-FGF Receptor (Tyr653/654) Antibody #3471 | Transfected† | Transfect with a construct overexpressing FGFR1 | |
| Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb #2544 | Transfected† | Transfect with a construct overexpressing FGFR1 | |
| Phospho-Filamin A (Ser2152) Antibody #4761 | 293 | Untreated | |
| Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb #3464 | SEM | Untreated | |
| Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb #3474 | SEM | Untreated | |
| Phospho-FLT3 (Tyr591) (54H1) Mouse mAb #3466 | SEM | Untreated | |
| Phospho-FLT3 (Tyr591) Antibody #3461 | SEM | Untreated | |
| Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb #4577 | SEM | Untreated | |
| Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb #3463 | SEM | Untreated | |
| Phospho-c-Fos (Ser32) (D82C12) XP® Rabbit mAb #5348 | HeLa | Serum-starve overnight, TPA (200 nM for 4 hrs) | |
| Phospho-FRA1 (Ser265) (D22B1) Rabbit mAb #5841 | HeLa | Serum-starve overnight, TPA (200 nM for 4 hrs) | |
| Phospho-FRA1 (Ser265) Antibody #3880 | HeLa | Serum-starve overnight, TPA (200 nM for 4 hrs) | |
| Phospho-FoxO1 (Ser256) Antibody #9461 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO1 (Ser319) Antibody #2486 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody #9464 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO1 (Ser319)/FoxO4 (Ser262) Antibody #2487 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO3a (Ser253) Antibody #9466 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO3a (Ser294) Antibody #5538 | C2C12 | H2O2 treated | |
| Phospho-FoxO3a (Ser318/321) Antibody #9465 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FoxO3a (Ser413) (D77C9) Rabbit mAb #8174 | SH-SY5Y | MG132-treated (10 μM for 4 hrs) | |
| Phospho-FoxO4 (Ser193) Antibody #9471 | HeLa | Serum-starve overnight, 20% serum for 30 min | |
| Phospho-FRS2-α (Tyr196) Antibody #3864 | NIH/3T3 | Serum-starve overnight, hFGF basic/FGF2 (100 ng/ml for 10 min) | |
| Phospho-FRS2-α (Tyr436) Antibody #3861 | NIH/3T3 | Serum-starve overnight, hFGF basic/FGF2 (100 ng/ml for 10 min) | |
| Phospho-Gab1 (Tyr307) Antibody #3234 | Hep G2 | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb #3233 | T-47D | Serum-starve overnight, neuregulin (100 ng/ml for 15 min) | |
| Phospho-Gab1 (Tyr627) Antibody #3231 | H-4-II-E | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Gab2 (Ser159) Antibody #3884 | H-4-II-E | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Gab2 (Tyr452) (C33G1) Rabbit mAb #3882 | T-47D | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Gab2 (Tyr452) Antibody #3881 | H-4-II-E | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Cleaved GGA3 (Asp313) (D16H3) Rabbit mAb #8068 | HeLa | Serum-starve overnight, Staurosporine (1µM for 3 hrs) | |
| Phospho-Glucocorticoid Receptor (Ser211) Antibody #4161 | A549 | Serum-starve overnight, dexamethasone (100 nM for 1 hr) | |
| Phospho-AMPA Receptor (GluR 1) (Ser845) (D10G5) Rabbit mAb #8084 | Rat brain, mouse brain | | |
| Phospho-AMPA Receptor (GluR 2) (Tyr869/Tyr873/Tyr876) Antibody #3921 | Rat brain | ischemia and reperfusion | |
| Phospho-AMPA Receptor (GluR 2) (Tyr876) Antibody #4027 | Rat brain | ischemia and reperfusion | |
| Phospho-Glycogen Synthase (Ser641) Antibody #3891 | 293 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-GRB10 (Tyr67) Antibody #3701 | H-4-II-E | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb #9316 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-GSK-3α (Ser21) (D1G2) Rabbit mAb #8452 | NIH/3T3 | PDGF (100 μg/ml for 5 min) | |
| Phospho-GSK-3α/β (Ser21/9) (37F11) Rabbit mAb (GSK-3α Preferred) #9327 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb #8566 | NIH/3T3 | PDGF (100 μg/ml for 5 min) | |
| Phospho-GSK-3α (Ser21) (46H12) Mouse mAb #9337 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-GSK-3α/β (Ser21/9) Antibody #9331 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb #5558 | NIH/3T3 | PDGF (100 μg/ml for 5 min) | |
| Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb #9323 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-GSK-3β (Ser9) (D3A4) Rabbit mAb #9322 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-GSK-3β (Ser9) Antibody #9336 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/ml for 30 min) | |
| Phospho-GSK-3β (Thr390) Antibody #3548 | HeLa | Paclitaxel (100 nM/ml for 20 hrs) | |
| Phospho-HDAC3 (Ser424) Antibody #3815 | NIH/3T3 | Untreated | |
| Phospho-HDAC4 (Ser246)/HDAC5 (Ser259)/HDAC7 (Ser155) (D27B5) Rabbit mAb #3443 | HeLa | Serum-starve overnight, Pervanadate | |
| Phospho-HDAC4 (Ser632)/HDAC5 (Ser498)/HDAC7 (Ser486) Antibody #3424 | HeLa | Serum-starve overnight, Pervanadate | |
| Phospho-HER2/ErbB2 (Tyr877) Antibody #2241 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER2/ErbB2 (Tyr1196) (D66B7) Rabbit mAb #6942 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb #2243 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER2/ErbB2 (Tyr1221/1222) Antibody #2249 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER2/ErbB2 (Tyr1248) Antibody #2247 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER2/ErbB2 (Tyr1248)/EGFR (Tyr1173) Antibody #2244 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER3/ErbB3 (Tyr1197) (C56E4) Rabbit mAb #4561 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER3/ErbB3 (Tyr1222) (50C2) Rabbit mAb #4784 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER3/ErbB3 (Tyr1289) (21D3) Rabbit mAb #4791 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER3/ErbB3 (Tyr1328) Antibody #8017 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER4/ErbB4 (Tyr984) Antibody #3790 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Phospho-HER4/ErbB4 (Tyr1284) (21A9) Rabbit mAb #4757 | MCF-7 | Serum-starve overnight, neuregulin (100 ng/ml for 5 min) | |
| Acetyl-Histone H2A (Lys5) Antibody #2576 | NIH/3T3 | TSA (400 nM overnight) | |
| Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718 | 293 | UV‡ (40 mJ/cm2), 2 hrs recovery | |
| Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb (PE Conjugate) #5763 | 293 | UV‡ (40 mJ/cm2), 2 hrs recovery | |
| Phospho-Histone H2A.X (Ser139) Antibody #2577 | 293 | UV‡ (40 mJ/cm2), 2 hrs recovery | |
| Phospho-Histone H2A.X (Ser139/Tyr142) Antibody #5438 | 293 | UV‡ (40 mJ/cm2), 2 hrs recovery | |
| Acetyl-Histone H2B (Lys5) Antibody #2574 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H2B (Lys12) Antibody #5410 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H2B (Lys15) Antibody #5435 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H2B (Lys20) Antibody #2571 | NIH/3T3 | TSA (400 nM overnight) | |
| Phospho-Histone H3 (Thr3) Antibody #9714 | NIH/3T3 | Serum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min) | |
| Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377 | NIH/3T3 | Serum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min) | |
| Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (PE Conjugate) #5764 | NIH/3T3 | Serum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min) | |
| Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 | NIH/3T3 | Serum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min) | |
| Phospho-Histone H3 (Ser10) Antibody #9701 | NIH/3T3 | Serum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min) | |
| Phospho-Histone H3 (Thr11) (C2A6) Rabbit mAb #9767 | NIH/3T3 | Serum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min) | |
| Phospho-Histone H3 (Thr11) Antibody #9764 | NIH/3T3 | Serum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min) | |
| Phospho-Histone H3 (Ser28) Antibody #9713 | HeLa | Serum-starve overnight, add 20% serum for 15 min, then Calyculin A (100 nM for 15 min) | |
| Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb #5326 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb #9725 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys4) Antibody #9726 | NIH/3T3 | Untreated | |
| Mono-Methyl-Histone H3 (Lys4) Antibody #9723 | NIH/3T3 | Untreated | |
| Pan-Methyl-Histone H3 (Lys9) (D54) XP® Rabbit mAb #4473 | NIH/3T3 | Untreated | |
| Pan-Methyl-Histone H3 (Lys9) Antibody #4069 | NIH/3T3 | Untreated | |
| Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733 | NIH/3T3 | Untreated | |
| Acetyl-Histone H3 (Lys27) Antibody #4353 | NIH/3T3 | TSA (400 nM overnight) | |
| Tri-Methyl-Histone H3 (Lys27) Antibody #9756 | NIH/3T3 | Untreated | |
| Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody #9711 | NIH/3T3 | Serum-starve plus 400 nM TSA overnight, add 20% serum for 15 min, then 100 nM Calyculin A for 15 min | |
| Acetyl-Histone H3 (Lys9) Antibody #9671 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H3 (Lys9/Lys14) Antibody #9677 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H3 (Lys14) Antibody #4318 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H3 (Lys14) Antibody (ChIP Formulated) #5275 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H3 (Lys18) Antibody #9675 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H3 (Lys56) Antibody #4243 | NIH/3T3 | TSA (400 nM overnight) | |
| Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb #4658 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys9) Antibody #9753 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb #9728 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys27) Antibody #9755 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb #2901 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys36) Antibody #9758 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys79) (D15E8) XP® Rabbit mAb #5427 | NIH/3T3 | Untreated | |
| Di-Methyl-Histone H3 (Lys79) Antibody #9757 | NIH/3T3 | Untreated | |
| Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751 | NIH/3T3 | Untreated | |
| Tri-Methyl-Histone H3 (Lys4) Antibody #9727 | NIH/3T3 | Untreated | |
| Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb #5327 | NIH/3T3 | Untreated | |
| Tri-Methyl-Histone H3 (Lys36) (D5A7) XP® Rabbit mAb #4909 | NIH/3T3 | Untreated | |
| Tri-Methyl-Histone H3 (Lys36) Antibody #9763 | NIH/3T3 | Untreated | |
| Mono-Methyl-Histone H4 (Lys20) Antibody #9724 | NIH/3T3 | Untreated | |
| Acetyl-Histone H4 (Lys5) Antibody #9672 | NIH/3T3 | TSA (400 nM overnight) | |
| Acetyl-Histone H4 (Lys8) Antibody #2594 | NIH/3T3 | TSA (400 nM overnight) | |
| Di-Methyl-Histone H4 (Lys20) Antibody #9759 | NIH/3T3 | Untreated | |
| Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb #5737 | NIH/3T3 | Untreated | |
| Phospho-HP1γ (Ser83) Antibody #2600 | HeLa | IBMX (30 µM) plus Forskolin (500 µM for 30 min) | |
| Phospho-HS1 (Tyr397) Antibody #4507 | Ramos | Serum-starve overnight, IgM (12 µg/ml for 10 min) | |
| Phospho-HSL (Ser563) Antibody #4139 | differentiated 3T3-L1 | Isoproterenol (10 µM for 20 min) | |
| Phospho-HSL (Ser565) Antibody #4137 | differentiated 3T3-L1 | Isoproterenol (10 µM for 20 min) | |
| Phospho-HSL (Ser660) Antibody #4126 | differentiated 3T3-L1 | Isoproterenol (10 µM for 20 min) | |
| Phospho-HSP27 (Ser15) Antibody #2404 | HeLa | Serum-starve overnight, UV‡ (40 mJ/cm2), 30 min recovery | |
| Phospho-HSP27 (Ser78) Antibody #2405 | HeLa | Serum-starve overnight, UV‡ (40 mJ/cm2), 30 min recovery | |
| Phospho-HSP27 (Ser82) Antibody #2401 | HeLa | Serum-starve overnight, UV‡ (40 mJ/cm2), 30 min recovery | |
| Phospho-HSP27 (Ser82) Antibody II #2406 | HeLa | Serum-starve overnight, UV‡ (40 mJ/cm2), 30 min recovery | |
| Phospho-HSP90α (Thr5/7) Antibody #3488 | HeLa | Serum-starve overnight, UV‡ (40 mJ/cm2), 30 min recovery | |
| Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb #4568 | MCF-7 | Serum-starve overnight, hIGF-I (100 ng/ml for 5 min) | |
| Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody #3021 | H-4-II-E | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IGF-I Receptor β (Tyr1316) Antibody #6113 | 293 | Serum-starve overnight, hIGF-I (50 ng/mL for 5 min) | |
| Phospho-IGF-1 Receptor β (Tyr1135) (DA7A8) Rabbit mAb #3918 | MCF-7 | Serum-starve overnight, hIGF-I (50 ng/mL for 5 min) | |
| Phospho-IGF-I Receptor β (Tyr1135/1136)/Insulin Receptor β (Tyr1150/1151) (19H7) Rabbit mAb #3024 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 | HeLa | hTNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246 | HeLa | hTNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-IκBβ (Thr19/Ser23) Antibody (Human Specific) #4921 | HeLa | hTNF-α (20 ng/ml) plus Calyculin A (50 nM for 5 min) | |
| Phospho-IκBε (Ser18/22) Antibody #4924 | HeLa | hTNF-α (20 ng/ml) plus Calyculin A (50 nM for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697 | 293 | hTNF-α (20 ng/ml for 10 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-IKKα/β (Ser176/180) Antibody II #2694 | HeLa | hTNF-α (20 ng/ml for 10 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-IKKγ (Ser376) Antibody #2689 | HeLa | hTNF-α (20 ng/ml for 10 min) | NF-κB Control Cell Extracts #9243 |
| Cleaved IL-1β (Asp116) Antibody #2021 | THP-1 | Differentiate with TPA (80 nM for 24 hrs), rest for 24 hrs, then treat with LPS (1 µg/ml for 24 hrs). Protein is secreted into media. | |
| Phospho-Insulin Receptor β (Tyr1345) (14A4) Rabbit mAb #3026 | Transfected† | Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-Insulin Receptor β (Tyr1361) (84B2) Rabbit mAb #3023 | Transfected† | Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IP3 Receptor (Ser1756) Antibody #3760 | Mouse brain, rat brain | Untreated | |
| Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb #4947 | RAW264.7 | LPS (1 μg/ml for 2 hrs) | |
| Phospho-IRF-7 (Ser471/472) Antibody #5184 | Transfected† | Transfect with IRF-7 | |
| Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb #2491 | Transfected† | Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 15 min) | |
| Phospho-IRS-1 (Ser302) Antibody #2384 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser307) Antibody #2381 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb #5610 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser332/336) Antibody #2580 | Transfected† | Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min) | |
| Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb #3203 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser612) (L7B8) Mouse mAb #3193 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser612) Antibody #2386 | mIMCD-3 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser636/639) Antibody #2388 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Ser789) Antibody #2389 | Transfected† | Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min) | |
| Phospho-IRS-1 (Tyr895) Antibody #3070 | Transfected† | Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min) | |
| Phospho-IRS-1 (Ser1101) Antibody #2385 | Differentiated C2C12 (myotubes) | Serum-starve overnight, insulin (100 nM for 5 min) | |
| Phospho-IRS-1 (Tyr1222) Antibody #3066 | Transfected† | Transfect with IR/IRS-1, serum-starve overnight, insulin (100 nM for 10 min) | |
| Phospho-Jak1 (Tyr1022/1023) Antibody #3331 | HT-29 | Serum-starve overnight, IL-4 (100 ng/ml for 5–10 min) | |
| Phospho-Jak2 (Tyr221) Antibody #3774 | CTLL-2 | Untreated | |
| Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb #3776 | TF-1 | Serum-starve overnight, hGM-CSF (25 ng/ml for 15 min); hGM-CSF (2 ng/ml) should be included during growth and serum-starvation | |
| Phospho-c-Jun (Ser63) (54B3) Rabbit mAb #2361 | NIH/3T3 | UV‡ (40 mJ/cm2), 30 min recovery time | c-Jun Control Cell Extracts #9263 |
| Phospho-c-Jun (Ser63) II Antibody #9261 | NIH/3T3 | UV‡ (40 mJ/cm2), 30 min recovery time | c-Jun Control Cell Extracts #9263 |
| Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb #3270 | NIH/3T3 | UV‡ (40 mJ/cm2), 30 min recovery time | c-Jun Control Cell Extracts #9263 |
| Phospho-c-Jun (Ser73) Antibody #9164 | NIH/3T3 | UV‡ (40 mJ/cm2), 30 min recovery time | c-Jun Control Cell Extracts #9263 |
| Phospho-c-Jun (Thr91) Antibody #2303 | NIH/3T3 | UV‡ (40 mJ/cm2), 30 min recovery time | c-Jun Control Cell Extracts #9263 |
| Phospho-c-Jun (Thr93) Antibody #2993 | NIH/3T3 | Untreated; site is constitutively phosphorylated | c-Jun Control Cell Extracts #9263 |
| Phospho-c-Jun (Ser243) Antibody #2994 | NIH/3T3 | UV‡ (40 mJ/cm2), 30 min recovery time | c-Jun Control Cell Extracts #9263 |
| Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb #8053 | C6 | Anisomycin (25 ug/ml for 20 min) | p38 MAPK Control Cell Extracts #9213 |
| Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb #3073 | NCI-H526 | Serum-starve overnight, hSCF (100 ng/ml for 5 min) | |
| Phospho-c-Kit (Tyr719) Antibody #3391 | NCI-H526 | Serum-starve overnight, hSCF (100 ng/ml for 5 min) | |
| Phospho-Keratin 17 (Ser44) Antibody #3519 | HeLa | Nocodazole (100 ng/ml for 4 hrs) | |
| Phospho-KSR1 (Ser392) Antibody #4951 | HeLa | Untreated; site is constitutively phosphorylated | |
| Cleaved Lamin A (Small Subunit) (30H5) Mouse mAb #2036 | HeLa | Serum-starve overnight, Staurosporine (1.0 μM for 4 hrs) | |
| Cleaved Lamin A (Small Subunit) Antibody #2035 | HeLa | Serum-starve overnight, Staurosporine (1.0 μM for 4 hrs) | |
| Phospho-Lamin A/C (Ser22) Antibody #2026 | THP-1 | Nocadazole (1 μg/ml for 20 hrs) | |
| Cleaved Lamin A (Asp230) Antibody #2031 | HeLa | Serum-starve overnight, Staurosporine (1.0 μM for 4 hrs) | |
| Phospho-LAT (Tyr171) Antibody #3581 | Jurkat | Anti-CD3 (10 μg/ml for 2 min) | |
| Phospho-LAT (Tyr191) Antibody #3584 | Jurkat | Anti-CD3 (10 μg/ml for 2 min) | |
| Phospho-LATS1 (Thr1079) Antibody #9159 | HeLa | Okadaic Acid (1 μMM for 1 hr) | |
| Phospho-LATS1 (Ser909) Antibody #9157 | HeLa | TPA (200 nM for 30 min) | |
| LC3A (D50G8) XP® Rabbit mAb #4599 | HeLa, NIH/3T3, C6 | Chloroquine (50 μM for 20 hrs) | |
| LC3B (G40) Antibody #4108 | HeLa, NIH/3T3, C6 | Chloroquine (50 μM for 20 hrs) | |
| LC3B (D11) XP® Rabbit mAb #3868 | HeLa, NIH/3T3, C6 | Chloroquine (50 μM for 20 hrs) | |
| LC3B Antibody #2775 | HeLa, NIH/3T3, C6 | Chloroquine (50 μM for 20 hrs) | |
| Phospho-Lck (Tyr505) Antibody #2751 | Jurkat | Serum-starve overnight, H2O2 (2 mM for 2 min) | |
| Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody #3841 | Transfected† | Transfect with construct overexpressing LIMK1 | |
| Phospho-LKB1 (Thr189) Antibody #3054 | Transfected† | Transfect with construct overexpressing LKB1 | |
| Phospho-LKB1 (Ser334) Antibody #3055 | Transfected† | Transfect with construct overexpressing LKB1 | |
| Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb #3482 | Caki | TPA (200 nM for 30 min) | |
| Phospho-5-Lipoxygenase (Ser271) Antibody #3748 | Transfected† | Transfect cells with construct overexpressing 5-lipoxygenase | |
| Phospho-5-Lipoxygenase (Ser663) Antibody #3749 | Mouse brain, rat brain | Untreated | |
| Phospho-LRP6 (Ser1490) Antibody #2568 | HeLa | Wnt3a-conditioned media for 5 hrs (Conditioned media from L cells transfected with Wnt3a ligand) | |
| Phospho-Lyn (Tyr507) Antibody #2731 | Ramos | Serum-starve overnight, anti-IgM (12 μg/ml for 2 min) | |
| Phospho-MAP2 (Ser136) Antibody #4541 | PC-12 | Positive control: Nocodazole (1 µg/ml for 16 hrs); negative control: λ phosphatase (800 U/500 μl for 1 hr) | |
| Phospho-MAP2 (Thr1620/1623) Antibody #4544 | PC-12 | Positive control: Nocodazole (1 µg/ml for 16 hrs); negative control: λ phosphatase (800 U/500 μl for 1 hr) | |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 | Jurkat | TPA (200 nM for 10 min) | p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb #4377 | Jurkat | TPA (200 nM for 10 min) | p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb #4376 | Jurkat | TPA (200 nM for 10 min) | p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106 | Jurkat | TPA (200 nM for 10 min) | p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
| Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101 | Jurkat | TPA (200 nM for 10 min) | p44/42 MAPK (Erk1/2) Control Cell Extracts #9194 |
| Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316 | HeLa | Serum-starve overnight, UV‡ (40 mJ/cm2), 20 min recovery | |
| Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007 | HeLa | Serum-starve overnight, UV‡ (40 mJ/cm2), 20 min recovery | |
| Phospho-MAPKAPK-2 (Thr334) Antibody #3041 | HeLa | Serum-starve overnight, UV‡ (40 mJ/cm2), 20 min recovery | |
| Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 | C6 | Anisomycin (25 μg/ml for 20 min) | p38 MAP Kinase Control Cell Extracts #9213 |
| Phospho-p38 MAPK (Thr180/Tyr182)(12F8) Rabbit mAb #4631 | C6 | Anisomycin (25 μg/ml for 20 min) | p38 MAP Kinase Control Cell Extracts #9213 |
| Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb #9215 | C6 | Anisomycin (25 μg/ml for 20 min) | p38 MAP Kinase Control Cell Extracts #9213 |
| Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb (Biotinylated) #4092 | C6 | Anisomycin (25 μg/ml for 20 min) | p38 MAP Kinase Control Cell Extracts #9213 |
| Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb #9216 | C6 | Anisomycin (25 μg/ml for 20 min) | p38 MAP Kinase Control Cell Extracts #9213 |
| Phospho-p38 MAPK (Thr180/Tyr182) Antibody #9211 | C6 | Anisomycin (25 μg/ml for 20 min) | p38 MAP Kinase Control Cell Extracts #9213 |
| Phospho-(Thr) MAPK/CDK Substrate Mouse mAb #2321 | Jurkat | Nocadazole (1 μg/ml for 12 hrs) | |
| Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) #9391 | Jurkat | Nocadazole (1 µg/ml for 12 hrs) | |
| Phospho-MARCKS (Ser152/156) Antibody #2741 | HeLa, NIH/3T3 | Serum-starve overnight, TPA (200 nM for 15 min) | |
| Phospho-MARK Family (Activation Loop) Antibody #4836 | Raji, BaF3 | Untreated | |
| Phospho-Mcl-1 (Ser159/Thr163) Antibody #4579 | NCI-H929 | MG132 (10 μM for 2 hrs) | |
| Phospho-MDM2 (Ser166) Antibody #3521 | MCF-7 | hIGF-I (100 ng/ml for 15 min) | |
| Phospho-MEF2A (Ser408) Antibody #9737 | Transfected† | Transfect with construct overexpressing MEF2A | |
| Phospho-MEK1 (Thr286) Antibody #9127 | HeLa | Serum-starve overnight, Nocodazole (0.2 μg/ml for 16 hrs) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-MEK1 (Ser298) Antibody #9128 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb #9154 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) #3958 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb #2338 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-MEK1/2 (Ser217/221) Antibody #9121 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-Merlin (Ser518) Antibody #9163 | Rat brain, mouse brain, human brain | Untreated | |
| Phospho-Met (Tyr1003) (13D11) Rabbit mAb #3135 | HCC827 | Untreated | |
| Phospho-Met (Tyr1234/1235) (D26) XP® Rabbit mAb #3077 | HCC827 | Untreated | |
| Phospho-Met (Tyr1234/1235) (3D7) Rabbit mAb #3129 | H-4-II-E | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Met (Tyr1234/1235) Antibody #3126 | H-4-II-E | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Met (Tyr1349) (130H2) Rabbit mAb #3133 | H-4-II-E | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-Met (Tyr1349) Antibody #3121 | H-4-II-E | Serum-starve overnight, HGF (40 ng/ml for 10 min) | |
| Phospho-MKK3/MKK6 (Ser189/207) (22A8) Rabbit mAb #9236 | NIH/3T3 | UV‡ (40 mJ/cm2), 30 min recovery time | MKK3/MKK6 Control Cell Extracts #9233 |
| Phospho-MKK3/MKK6 (Ser189/207) Antibody #9231 | NIH/3T3 | UV‡ (40 mJ/cm2), 30 min recovery time | MKK3/MKK6 Control Cell Extracts #9233 |
| Phospho-MKK7 (Ser271/Thr275) Antibody #4171 | U-937 | Sorbitol (400 mM for 15 min) | |
| Phospho-Mnk1 (Thr197/202) Antibody #2111 | HeLa | Serum-starve overnight, 20% serum for 20 min or Anisomycin (25 µg/ml for 30 min) | p38 MAP Kinase Control Cell Extracts #9213 |
| Phospho-MKP1 (Ser359) (125E2) Rabbit mAb #2857 | HeLa | ALLN (50 µM for 5–6 hrs) | |
| Phospho-Mre11 (Ser676) Antibody #4859 | HeLa | UV‡ (100 mJ/cm2), 1 hr recovery | |
| Phospho-MSK1 (Ser360) Antibody #9594 | Transfected† | Transfect with construct overexpressing MSK1 | |
| Phospho-MSK1 (Ser376) Antibody #9591 | 293 | UV‡ (40 mJ/cm2), 30 min recovery time | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-MSK1 (Thr581) Antibody #9595 | 293 | UV‡ (40 mJ/cm2), 30 min recovery time | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-Mst1 (Thr183)/Mst2 (Thr180) Antibody #3681 | WEHI | Staurosporine (1 µM for 30 min – 3 hrs) | |
| Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb #5536 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific) #2976 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-mTOR (Ser2448) Antibody #2971 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-mTOR (Ser2481) Antibody #2974 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-Myosin IIa (Ser1943) Antibody #5026 | HeLa, A-431, 293T | Untreated | |
| Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody #3674 | C2C12 | Untreated | |
| Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb #3675 | C2C12 | Untreated | |
| Phospho-Myosin Light Chain 2 (Ser19) Antibody #3671 | C2C12 | Untreated | |
| Phospho-MYPT1 (Ser507) Antibody #3040 | HeLa | Serum-starve overnight, Calyculin A (100 nM for 30 min) | |
| Phospho-MYPT1 (Ser668) Antibody #3048 | HeLa | Serum-starve overnight, Calyculin A (100 nM for 30 min) | |
| Phospho-MYPT1 (Thr696) Antibody #5163 | 293 | Untreated | |
| Phospho-MYPT1 (Thr853) Antibody #4563 | HeLa, NIH/3T3 | Untreated | |
| Phospho-Myt1 (Ser83) Antibody #4281 | HeLa | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-NDRG1 (Ser330) Antibody #3506 | Jurkat | Calyculin A (100 nM, 20 min) | |
| Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb #5482 | C2C12 | Untreated | |
| Phospho-NDRG1 (Thr346) Antibody #3217 | C2C12 | Insulin, 30 min | |
| Phospho-NEDD4L (Ser342) Antibody #4080 | COS | Transfected with NEDD4L plasmid | |
| Phospho-NEDD4L (Ser448) Antibody #8063 | SK-MEL-28 | Untreated | |
| Phospho-NF-κB2 p100 (Ser866/870) Antibody #4810 | Transfected† | Cotransfect contructs overexpressing NIK and p100 | |
| Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb #4806 | HeLa | hTNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-NF-κB p65 (Ser468) Antibody #3039 | HeLa | hTNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 | HeLa | hTNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-NF-κB p65 (Ser536) (7F1) Mouse mAb #3036 | HeLa | hTNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Phospho-NF-κB p65 (Ser536) Antibody #3031 | HeLa | hTNF-α (20 ng/ml for 5 min) | NF-κB Control Cell Extracts #9243 |
| Acetyl-NF-κB p65 (Lys310) Antibody #3045 | Transfected† | Cotransfect constructs overexpressing p65 and CBP/p300 | |
| Phospho-NMDAR1 (Ser890) Antibody #3381 | Transfected† | Transfect with construct expressing NMDAR1 and treat with TPA (100 nM for 5 min) | |
| Phospho-NMDAR1 (Ser896) Antibody #3384 | Transfected† | Transfect with construct expressing NMDAR1 and treat with TPA (100 nM for 5 min) | |
| Phospho-NMDAR1 (Ser897) Antibody #3385 | Transfected† | Transfect with construct expressing NMDAR1 and treat with Forskolin (30 μM for 20 min) | |
| Phospho-NMDAR2A (Tyr1246) Antibody #4206 | Rat brain | Untreated | |
| Phospho-NMDAR2B (Tyr1070) Antibody #4209 | Rat brain | Untreated | |
| Phospho-NMDAR2B (Ser1284) Antibody #5355 | Fetal rat brain | | |
| Phospho-NMDAR2B (Tyr1472) Antibody #4208 | Rat brain | Untreated | |
| Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147 | MOLT-4, Jurkat | Untreated | |
| Cleaved Notch1 (Val1744) Antibody #2421 | MOLT-4, Jurkat | Untreated | |
| Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520 | HeLa | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-NPM (Thr95) Antibody #3517 | HeLa | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-NPM (Thr199) Antibody #3541 | HeLa | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-NuMA (Ser395) Antibody #3429 | M059K | UV‡ (100 mJ/cm2), 1 hr recovery | |
| Phospho-Numb (Ser276) (D5C2) Rabbit mAb #9878 | HeLa | TPA (200 nM for 15-30 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-Numb (Ser276) Antibody #4140 | Ramos | TPA (200 nM for 30 min) | |
| Phospho-Nur77 (Ser351) (D22G5) Rabbit mAb #5095 | Jurkat cells | TPA #4174 (40 nM), A23187 (2 µM) both for 4 hrs | |
| Phospho-mu-Opioid Receptor (Ser375) Antibody #3451 | Transfected† | Transfect 293 cells with mu-opioid receptor and treat with DAMGO (1 mM for 5 min) | |
| Phospho-p130 Cas (Tyr165) Antibody #4015 | NIH/3T3 | Untreated | |
| Phospho-p130 Cas (Tyr249) Antibody #4014 | NIH/3T3 | Untreated | |
| Phospho-p130 Cas (Tyr410) Antibody #4011 | NIH/3T3 | Untreated | |
| Phospho-p40phox (Thr154) Antibody #4311 | THP-1 | Serum-starve overnight, TPA (200 nM for 15 min) | |
| Phospho-p53 (Ser6) Antibody #9285 | HT29, Cos, 293, MCF7 | UV‡ (75 mJ/cm2), 0.5 to 2 hrs recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh media | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-p53 (Ser9) Antibody #9288 | HT29, Cos, 293, MCF7 | UV‡ (75 mJ/cm2), 0.5 to 2 hrs recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh media | |
| Phospho-p53 (Ser15) (16G8) Mouse mAb #9286 | HT29, Cos, 293, MCF7 | UV‡ (75 mJ/cm2), 0.5 to 2 hrs recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh media | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-p53 (Ser15) Antibody #9284 | HT29, Cos, 293, MCF7 | UV‡ (75 mJ/cm2), 0.5 to 2 hrs recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh media | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-p53 (Thr18) Antibody #2529 | HT29, Cos, 293, MCF7 | Doxorubicin (0.5 µM for 24 hrs) | |
| Phospho-p53 (Ser20) Antibody #9287 | HT29, Cos, 293, MCF7 | UV‡ (75 mJ/cm2), 0.5 to 2 hrs recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh media | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-p53 (Ser33) Antibody #2526 | HT29, Cos, 293, MCF7 | Nocodazole (50 ng/ml for 24–48 hrs) | |
| Phospho-p53 (Ser37) Antibody #9289 | HT29, Cos, 293, MCF7 | UV‡ (75 mJ/cm2), 0.5 to 2 hrs recovery, 10 µM Etoposide (timing varies), 0.5 µM Doxorubicin (timing varies), 1 µM Camptothecin (timing varies), IR (10 Gy) wth recovery in fresh media | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-p53 (Ser46) Antibody #2521 | HT29, Cos, 293, MCF7 | UV‡ (75 mJ/cm2), 2 hrs recovery | |
| Phospho-p53 (Thr81) Antibody #2676 | HeLa | Nocodazole (50 ng/ml for 24–48 hrs) | |
| Phospho-p53 (Ser315) Antibody #2528 | HT29, Cos, 293, MCF7 | Nocodazole (50 ng/ml for 24–48 hrs) | |
| Phospho-p53 (Ser392) Antibody #9281 | HT29, Cos, 293, MCF7 | Hydroxyurea (20 mM for 30 hrs) | |
| Acetyl-p53 (Lys379) Antibody #2570 | HT29, Cos, 293, MCF7 | Doxorubicin (0.5 µM for 24 hrs); 400 nM TSA can be used in conjunction to stabilize activated protein | |
| Acetyl-p53 (Lys382) Antibody #2525 | HT29, Cos, 293, MCF7 | Doxorubicin (0.5 µM for 24 hrs); 400 nM TSA can be used in conjunction to stabilize activated protein | |
| Phospho-p57 Kip2 (Thr310) Antibody #2558 | HeLa | Dexamethasone (50 nM for 16 hrs) | |
| Phospho-p63 (Ser160/162) Antibody #4981 | ME-180 | Nocodazole (100 ng/ml for 18 hrs) | |
| Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb #9206 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-p70 S6 Kinase (Thr389) Antibody #9205 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-p70 S6 Kinase (Ser371) Antibody #9208 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-Drosophila p70 S6 Kinase (Thr398) Antibody #9209 | S2 | hEGF (100 ng/ml for 30 min) | |
| Phospho-p70 S6 Kinase (Thr421/Ser424) Antibody #9204 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-p73 (Tyr99) Antibody #4665 | HT-1376 | Pervanadate (1 mM for 20 min) | |
| Phospho-p90RSK (Thr359/Ser363) Antibody #9344 | HeLa | TPA (200 nM for 30 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-p95/NBS1 (Ser343) Antibody #3001 | HeLa | UV‡ (40 mJ/cm2), 90 min recovery | |
| Methyl-PABP1 (Arg455/Arg460) (C60A10) Rabbit mAb #3505 | HeLa, C6, COS | Untreated | |
| Phospho-PAK1 (Ser144)/PAK2 (Ser141) Antibody #2606 | Guinea pig neutrophils | fMLP (1 µM for 30 seconds) | |
| Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody #2605 | Guinea pig neutrophils | fMLP (1 µM for 30 seconds) | |
| Phospho-PAK1 (Thr423)/PAK2 (Thr402) Antibody #2601 | Guinea pig neutrophils | fMLP (1 µM for 30 seconds) | |
| Phospho-PAK2 (Ser20) Antibody #2607 | Guinea pig neutrophils | fMLP (1 µM for 30 seconds) | |
| Phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560) Antibody #3241 | Guinea pig neutrophils | fMLP (1 µM for 30 seconds) | |
| Phospho-PAR-4 (Thr163) Antibody #2329 | HeLa | Calyculin A (100 nM for 5 min) | |
| Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) #9548 | L-929 | Serum-starve overnight, Staurosporine (1 µM for 3 hrs) | |
| Cleaved PARP (Asp214) Antibody (Mouse Specific) #9544 | L-929 | Serum-starve overnight, (1 µM Staurosporine for 3 hrs) | |
| Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 | HeLa | Serum-starve overnight, Staurosporine (1 µM for 3 hrs) | |
| Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) #9546 | HeLa | Serum-starve overnight, Staurosporine (1 µM for 3 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
| Cleaved PARP (Asp214) Antibody (Human Specific) #9541 | HeLa | Serum-starve overnight, Staurosporine (1 µM for 3 hrs) | Jurkat Apoptosis Cell Lysates (etoposide) #2043 |
| Cleaved PARP (Asp214) Antibody (Rat Specific) #9545 | C6 | Serum-starve overnight, (1 µM Staurosporine for 3 hrs) | |
| Phospho-Paxillin (Tyr118) Antibody #2541 | C2C12, NIH/3T3, A-431, C6 | Untreated | |
| Phospho-PBK/TOPK (Thr9) Antibody #4941 | HT-29 | Nocodazole (50 ng/ml for 20 hrs) | |
| Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb #2992 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) Rabbit mAb #3170 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor α (Tyr1018) Antibody #4547 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr740) (32A9) Rabbit mAb #3168 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr751) (C63G6) Rabbit mAb #4549 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr751) (88H8) Mouse mAb #3166 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr751) Antibody #3161 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr771) (76D6) Rabbit mAb #3173 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr1009) (42F9) Rabbit mAb #3124 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-PDGF Receptor β (Tyr1021) (6F10) Rabbit mAb #2227 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-(Ser/Thr) PDK1 Docking Motif (18A2) Mouse mAb #9634 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb #3438 | Jurkat | Untreated | |
| Phospho-PDK1 (Ser241) Antibody #3061 | HeLa | Untreated | |
| Phospho-PEA-15 (Ser104) Antibody #2776 | C6 | TPA (200 nM for 1 hr) | |
| Phospho-PERK (Thr980) (16F8) Rabbit mAb #3179 | AR42J | Thapsigargin (1 μM for 20 min) | |
| Phospho-(Ser/Thr) Phe Antibody #9631 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody #4228 | NIH/3T3/Src | Untreated | |
| Phospho-Pin1 (Ser16) Antibody #3721 | OVCAR-8 | Forskolin (30 µM for 30 min) | |
| Phospho-PKA C (Thr197) (D45D3) Rabbit mAb #5661 | NIH/3T3 | Untreated | |
| Phospho-PKA Substrate (RRXS/T) (100G7E) Rabbit mAb #9624 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-(Ser/Thr) PKA Substrate Antibody #9621 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-PKCα/β II (Thr638/641) Antibody #9375 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/mL for 30 min) | |
| Phospho-PKCδ (Tyr311) Antibody #2055 | C6 | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-PKCδ (Thr505) Antibody #9374 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/mL for 30 min) | |
| Phospho-PKCδ/θ (Ser643/676) Antibody #9376 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/mL for 30 min) | |
| Phospho-PKC (pan) (γ Thr514) Antibody #9379 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/mL for 30 min) | |
| Phospho-PKC (pan) (βII Ser660) Antibody #9371 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/mL for 30 min) | |
| Phospho-PKC (pan) (ζ Thr410) (190D10) Rabbit mAb (Biotinylated) #5016 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/mL for 30 min) | |
| Phospho-PKC (pan) (ζ Thr410) (190D10) Rabbit mAb #2060 | COS | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-PKCθ (Thr538) Antibody #9377 | HeLa | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-PKCζ/λ (Thr410/403) Antibody #9378 | 293 | Serum-starve overnight, hEGF (100 ng/mL for 10 min) | |
| Phospho-(Ser/Thr) PKD Substrate Antibody #4381 | HeLa | TPA (200 nM for 30 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PKD/PKCμ (Ser744/748) Antibody #2054 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/mL for 30 min) | |
| Phospho-PKD/PKCμ (Ser916) Antibody #2051 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/mL for 30 min) | |
| Phospho-cPLA2 (Ser505) Antibody #2831 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PLCβ3 (Ser537) Antibody #2481 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PLCβ3 (Ser1105) Antibody #2484 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PLCγ1 (Tyr783) Antibody #2821 | NIH/3T3 | Serum-starve overnight, PDGF (50 ng/ml for 30 min) | |
| Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb #8713 | NIH/3T3 | hPDGF-BB (50 ng/mL for 15 min) | |
| Phospho-PLCγ1 (Ser1248) Antibody #4510 | COS | TPA (200 nM for 20 min) | |
| Phospho-PLCγ2 (Tyr759) Antibody #3874 | Ramos, Raji | IgM (12 µg/ml for 2 min) | |
| Phospho-PLCγ2 (Tyr1217) Antibody #3871 | Ramos, Raji | IgM (12 µg/ml for 2 min) | |
| Phospho-PLD1 (Thr147) Antibody #3831 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PLD1 (Ser561) Antibody #3834 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-PNK1 (Ser114/Thr118) Antibody #3522 | HeLa | UV‡ (100 mJ/cm2), 1 hr recovery | |
| Phospho-PP1α (Thr320) Antibody #2581 | HeLa, COS, NIH/3T3, C6 | Untreated | |
| Phospho-PPIG (Ser374) Antibody #3804 | Jurkat | Untreated | |
| Phospho-PRAS40 (Ser183) Antibody #5936 | HeLa | Insulin (100 nM for 5 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb #2997 | NIH/3T3 | Serum-starve overnight, insulin (100 nM for 5–10 min) | |
| Phospho-PRAS40 (Thr246) Antibody #2640 | NIH/3T3 | Serum-starve overnight, insulin (100 nM for 5–10 min) | |
| Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody #2611 | Jurkat | Untreated or Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Progesterone Receptor (Ser190) Antibody #3171 | T-47D | Serum-starve overnight, promegestone (100 nM for 1 hr) | |
| Phospho-PSD93 (Tyr340) Antibody #2930 | Rat brain | Untreated | |
| Phospho-PSD95 (Tyr236/Tyr240) Antibody #3919 | Rat brain | Untreated | |
| Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb #9549 | HeLa | Untreated | |
| Phospho-PTEN (Ser380) Antibody #9551 | HeLa | Untreated | |
| Phospho-PTEN (Ser380/Thr382/383) Antibody #9554 | HeLa | Untreated | |
| Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 | Jurkat | Pervanadate (1 mM for 30 min) | |
| Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) #5465 | Jurkat | Pervanadate (1 mM for 30 min) | |
| Immobilized Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9419 | Jurkat | Pervanadate (1 mM for 30 min) | |
| Phospho-Tyrosine Mouse mAb (P-Tyr-102) #9416 | Jurkat | Pervanadate (1 mM for 30 min) | |
| Phospho-PLK Binding Motif (ST*P) (D73F6) Rabbit mAb #5243 | HeLa | nocodazole (100 ng/ml for 16 hr) | |
| Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb #6967 | HeLa | TPA (200 nM for 30 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb #2325 | HeLa | Nocadazole (1 μg/ml for 12 hrs) | |
| Phospho-(Ser) CDKs Substrate Antibody #2324 | HeLa | Nocadazole (1 μg/ml for 12 hrs) | |
| Phospho-(Ser) PKC Substrate Antibody #2261 | HeLa | TPA (200 nM for 30 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-Threonine-X-Arginine Antibody #2351 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-Thr-Pro-Glu (C32G12) Rabbit mAb #3004 | HeLa | nocodazole (100 ng/ml for 16 hr) | |
| Phospho-PTPα (Tyr789) Antibody #4481 | 293, COS, C6, C2C12 | Untreated | |
| Phospho-Pyk2 (Tyr402) Antibody #3291 | Jurkat | Serum-starve overnight, anti-CD3 plus anti-CD28 (both 1 µg/ml for 10 min) | |
| Phospho-PZR (Tyr241) (D6F9) Rabbit mAb #8131 | BAEC | H2O2 (0.3 mM for 30 min) | |
| Phospho-PZR (Tyr263) (D6A5) Rabbit mAb #8088 | BAEC | H2O2 (0.3 mM for 30 min) | |
| Phospho-PZR (Tyr263) Antibody #5543 | BAEC | H2O2 (0.3 mM for 30 min) | |
| Phospho-Rac1/cdc42 (Ser71) Antibody #2461 | A-431 | Serum-starve overnight, hEGF (100 ng/ml for 10 min) | |
| Phospho-A-Raf (Ser299) Antibody #4431 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-B-Raf (Ser445) Antibody #2696 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-c-Raf (Ser259) Antibody #9421 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-c-Raf (Ser289/296/301) Antibody #9431 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-c-Raf (Ser296) Antibody #9423 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-c-Raf (Ser338) (56A6) Rabbit mAb #9427 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-RanBP3 (Ser58) Antibody #9380 | HeLa | Serum-starve overnight, hEGF (100 ng/mL for 10 min) | |
| Phospho-Raptor (Ser792) Antibody #2083 | 293 | Oligomycin (0.5 µM for 30 min) | |
| Phospho-Ras-GRF1 (Ser916) Antibody #3321 | Transfected† | Transfect with construct overexpressing Ras-GRF1 | |
| Phospho-Rb (Ser608) Antibody #2181 | HT-29 | Nocodazole (100 ng/ml for 18 hrs); treatment induces slight phospho-shift | |
| Phospho-Rb (Ser780) (C84F6) Rabbit mAb #3590 | HT-29 | Nocodazole (100 ng/ml for 18 hrs) - treatment induces slight phospho-shift | |
| Phospho-Rb (Ser780) Antibody #9307 | HT-29 | Nocodazole (100 ng/ml for 18 hrs); treatment induces slight phospho-shift | Rb Control Proteins #9303 |
| Phospho-Rb (Ser795) Antibody #9301 | HT-29 | Nocodazole (100 ng/ml for 18 hrs); treatment induces slight phospho-shift | Rb Control Proteins #9303 |
| Phospho-Rb (Ser807/811) Antibody #9308 | HT-29 | Nocodazole (100 ng/ml for 18 hrs); treatment induces slight phospho-shift | Rb Control Proteins #9303 |
| Phospho-RCC1 (Ser11) Antibody #4210 | HT-29 | Thymidine (2 mM for 16 hrs) followed by Nocodazole (100 ng/ml for 24 hrs) | |
| Phospho-RelB (Ser552) (D41B9) XP® Rabbit mAb #5025 | Raji | TPA (200 nM for 30 min) | |
| Phospho-RelB (Ser552) Antibody #4999 | Jurkat | TPA (200 nM for 30 min) | |
| Phospho-REPS1 (Ser709) Antibody #6884 | MCF-7 | Untreated | |
| Phospho-Ret (Tyr905) Antibody #3221 | TT | Untreated | |
| Phospho-Rev-erbα (Ser55/59) Antibody #2129 | Transfected† | Transfect with a construct overexpressing Rev-Erbα | |
| Phospho-Rictor (Thr1135) (D30A3) Rabbit mAb #3806 | HeLa | Serum-starve overnight, hIGF-I (50 ng/ml for 30 min) | |
| Phospho-RIP2 (Ser176) Antibody #4364 | THP-1 | Treat overnight with TPA (50 ng/ml), then culture in fresh medium 48 hrs to form differentiated macrophages. Induce with LPS (1 µg/ml for 10 min). Dorsch, M. et al. (2006) Cell. Signal. 18, 2223-2229. | |
| Phospho-Ros (Tyr2274) Antibody #3078 | HCC78 | HCC78 cells express a SLC3482-Ros fusion protein | |
| Phospho-Rpb1 CTD (Ser2/5) Antibody #4735 | MCF-7 | Doxorubicin (0.5 µM for 30 hrs) | |
| Phospho-p90RSK (Ser380) (9D9) Rabbit mAb #9335 | HeLa | TPA (200 nM for 30 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-p90RSK (Ser380) Antibody #9341 | HeLa | TPA (200 nM for 30 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-p90RSK (Thr573) Antibody #9346 | HeLa | TPA (200 nM for 30 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb #3556 | HeLa | TPA (200 nM for 30 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-RSK2 (Tyr529) Antibody #9324 | SK-N-MC | Serum-starve overnight, FGF (100 ng/ml for 10 min) | |
| Phospho-RSK3 (Thr356/Ser360) Antibody #9348 | HeLa | Serum-starve overnight, TPA (200 nM for 15 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb #4858 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Biotinylated) #3945 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (PE Conjugate) #5316 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb #4856 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb #4857 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-S6 Ribosomal Protein (Ser235/236) Antibody #2211 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb #5364 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-S6 Ribosomal Protein (Ser240/244) (61H9) Rabbit mAb (Biotinylated) #4278 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-S6 Ribosomal Protein (Ser240/244) Antibody #2215 | MCF-7 | Serum-starve overnight, insulin (100 nM for 5–10 min) | p70 S6 Kinase Control Cell Extracts #9203 |
| Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 | 293 | UV‡ (40 mJ/cm2), 30 min recovery time | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb #4671 | 293 | UV‡ (40 mJ/cm2), 30 min recovery time | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb #9255 | 293 | UV‡ (40 mJ/cm2), 30 min recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-SAPK/JNK(Thr183/Tyr185) (G9) Mouse mAb (PE Conjugate) #5755 | 293 | UV‡ (40 mJ/cm2), 30 min recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-SAPK/JNK (Thr183/Tyr185) Antibody #9251 | 293 | UV‡ (40 mJ/cm2), 30 min recovery | SAPK/JNK Control Cell Extracts #9253 |
| Phospho-SATB1 (Ser47) Antibody #4028 | Jurkat | Untreated | |
| Phospho-SEK1/MKK4 (Ser80) Antibody #9155 | NIH/3T3 | PDGF (100 ng/ml for 5 min) | |
| Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb #4514 | HeLa | UV‡ (50 mJ/cm2), 30 min recovery | |
| Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody #9156 | HeLa | UV‡ (50 mJ/cm2), 30 min recovery | |
| Phospho-SEK1/MKK4 (Thr261) Antibody #9151 | HeLa | UV‡ (50 mJ/cm2), 30 min recovery | |
| Phospho-Semaphorin 4B (Ser825) Antibody #5622 | MKN-45 | Untreated | |
| Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody #2981 | Jurkat | Calyculin A (100 nM for 30 min) | Akt Control Cell Extracts #9273 |
| Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb #5599 | SK-MEL-28 | H2O2 (4 mM for 15 min) | |
| Phospho-SGK (Ser78) Antibody #3271 | Transfected† | Transfect with construct overexpressing SGK | |
| Phospho-SGK3 (Thr320) (D30E6) Rabbit mAb #5642 | MCF7 | H2O2 (4 mM for 15 min) | |
| Phospho-Shc (Tyr239/240) Antibody #2434 | Hep G2 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | |
| Phospho-Shc (Tyr317) Antibody #2431 | Hep G2 | Serum-starve overnight, hEGF (100 ng/ml for 5 min) | |
| Phospho-SHIP1 (Tyr1020) Antibody #3941 | Ramos | Anti-human IgM (12 µg/ml for 2 min) | |
| Phospho-SHIP2 (Tyr986/987) Antibody #2008 | K-562 | Untreated | |
| Phospho-SHIP2 (Tyr1135) (D33C6) XP® Rabbit mAb #5445 | K-562 | Untreated | |
| Phospho-SHIP2 (Tyr1135) Antibody #2007 | K-562 | Untreated | |
| Phospho-SHP-2 (Tyr542) Antibody #3751 | NIH/3T3 | Serum-starve overnight, PDGF (100 ng/ml for 5 min) | |
| Phospho-SHP2 (Tyr580) (D66F10) Rabbit mAb #5431 | C6 cells | Serum-starved overnight and treated with PDGF-BB, 50 ng/ml for 5 minutes | |
| Phospho-SHP-2 (Tyr580) Antibody #3703 | NIH/3T3 | Serum-starve overnight, PDGF-β (100 ng/ml for 5 min) | |
| Phospho-SirT1 (Ser27) Antibody #2327 | 293 | Untreated | |
| Phospho-SirT1 (Ser47) Antibody #2314 | 293 | Untreated | |
| Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 | HeLa, HT-1080, C2C12, C6 | Serum-starve overnight, BMP (50 ng/ml for 30 min) | |
| Phospho-Smad1 (Ser206) (D40B7) Rabbit mAb #5753 | HeLa | Serum-starve overnight, UV‡ (50 mJ/cm2), 1 hr recovery | |
| Phospho-Smad1 (Ser206) Antibody #9553 | HeLa | Serum-starve overnight, UV‡ (50 mJ/cm2), 1 hr recovery | |
| Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad8 (Ser426/428) Antibody #9511 | HeLa, HT-1080, C2C12, C6 | Serum-starve overnight, BMP (50 ng/ml for 30 min) | |
| Phospho-Smad2 (Ser245/250/255) Antibody #3104 | HeLa | Serum-starve overnight, TPA (200 nM for 30 min) | |
| Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb #3108 | HeLa, HT-1080, C2C12, KNRK | Serum-starve overnight, TGF-β (10 ng/ml for 30 min) | |
| Phospho-Smad2 (Ser465/467) Antibody #3101 | HeLa, HT-1080, C2C12, KNRK | Serum-starve overnight, TGF-β (10 ng/ml for 30 min) | |
| Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP® Rabbit mAb #9510 | HeLa | Serum-starve overnight, TGF-β (10 ng/ml for 30 min) | |
| Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb #9520 | HT-1080, C2C12, KNRK | Serum-starve overnight, TGF-β (10 ng/ml for 30 min) | |
| Phospho-SMC1 (Ser360) Antibody #4029 | 293 | UV‡ (100 mJ/cm2), 4 hrs recovery | |
| Phospho-SMC1 (Ser957) (5D11G5) Mouse mAb #4805 | Raw | UV‡ (100 mJ/cm2), 1 hr recovery | |
| Phospho-Src (Ser17) Antibody #5473 | MCF-7 | Untreated | |
| Phospho-Src Family (Tyr416) (100F9) Rabbit mAb #2113 | COLO201 | Serum-starve overnight, treat 5 min 20% FBS | |
| Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943 | COLO201 | Untreated | |
| Phospho-Src Family (Tyr416) Antibody #2101 | COLO201 | Untreated | |
| Phospho-Src (Tyr527) Antibody #2105 | COLO201 | Untreated | |
| Phospho-SRC-3 (Thr24) Antibody #2979 | MCF-7 | Untreated | |
| Phospho-SRF (Ser103) Antibody #4261 | HeLa | Serum-starve overnight, TPA (15 min at 200 nM) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat1 Control Cell Extracts #9173 |
| Phospho-Stat1 (Tyr701) Antibody #9171 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat1 Control Cell Extracts #9173 |
| Phospho-Stat1 (Ser727) Antibody #9177 | 293 | UV‡ (40 mJ/cm2), 30 min recovery | |
| Phospho-Stat2 (Tyr690) Antibody #4441 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 15 min) | |
| Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb (Biotinylated) #4093 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat3 Control Cell Extracts #9133 |
| Phospho-Stat3 (Tyr705) (3E2) Mouse mAb #9138 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat3 Control Cell Extracts #9133 |
| Phospho-Stat3 (Tyr705) (M9C6) Mouse mAb #4113 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat3 Control Cell Extracts #9133 |
| Phospho-Stat3 (Tyr705) Antibody #9131 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat3 Control Cell Extracts #9133 |
| Phospho-Stat3 (Ser727) (6E4) Mouse mAb #9136 | A-431 | Serum-starve overnight, hEGF (100 ng/ml for 5–10 min) | |
| Phospho-Stat3 (Ser727) Antibody #9134 | A-431 | Serum-starve overnight, hEGF (100 ng/ml for 5–10 min) | |
| Acetyl-Stat3 (Lys685) Antibody #2523 | Transfected† | Cotransfect with contructs expressing Stat3 and CBP (p300/CREB Binding Protein) | |
| Phospho-Stat4 (Tyr693) Antibody #5267 | NK-92 | Cytokine-starve overnight, IL-2 (10 ng/ml for 15 min); IL-2 (5 ng/ml) should be included during growth | |
| Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb #4322 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat5 Control Cell Extracts #9353 |
| Phospho-Stat5 (Tyr694) (C11C5) Rabbit mAb #9359 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat5 Control Cell Extracts #9353 |
| Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat5 Control Cell Extracts #9353 |
| Phospho-Stat5 (Tyr694) (14H2) Mouse mAb #9356 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat5 Control Cell Extracts #9353 |
| Phospho-Stat5 (Tyr694) Antibody #9351 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 5 min) | Stat5 Control Cell Extracts #9353 |
| Phospho-Stat6 (Tyr641) (C11A12) Rabbit mAb #9364 | HeLa | Serum-starve overnight, IL-4 (100 ng/ml for 15 min) | |
| Phospho-Stat6 (Tyr641) Antibody #9361 | HeLa | Serum-starve overnight, IL-4 (100 ng/ml for 15 min) | |
| Phospho-Stathmin (Ser16) Antibody #3353 | HeLa | Nocadazole (100 ng/ml for 16 hrs) | |
| Phospho-Stathmin (Ser38) (D19H10) Rabbit mAb #4191 | HT-29 | Thymidine (2 mM for 16 hrs) followed by Nocodazole (100 ng/ml for 24 hrs) | |
| Phospho-Stathmin (Ser38) Antibody #3426 | HeLa | Nocadazole (100 ng/ml for 16 hrs) | |
| Phospho-Survivin (Thr34) (D2E11) Rabbit mAb #8888 | HeLa | Thymidine (2 mM for 16 hrs) followed by Nocodazole (100 mg/ml for 24 hrs) | |
| Phospho-Syk (Tyr323) Antibody #2715 | Ramos | Serum-starve overnight, anti-IgM (10 ug/ml for 2–10 min) | |
| Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb #2710 | Ramos | Serum-starve overnight, anti-IgM (10 ug/ml for 2–10 min) | |
| Phospho-Syk (Tyr525/526) Antibody #2711 | Ramos | Serum-starve overnight, IgM (12 µg/ml for 10 min) | |
| Phospho-Synapsin (Ser9) Antibody #2311 | PC-12 | NGF (100 ng/ml for 2 hrs) | |
| Phospho-TAB2 (Ser372) (D5A4) Rabbit mAb #8155 | 293 | Untreated | |
| Phospho-TACC3 (Ser558) Antibody #5645 | HT-29 | Thymidine (2mM 16 hrs), add fresh media and Nocodazole (100ng/ml 24 hrs), colllect by mitotic shake off | |
| Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb #4508 | HeLa | hTNF-α or IL-1β (20 ng/ml), both with Calyculin A (100 nM) for 10 min | |
| Phospho-TAK1 (Thr184) Antibody #4537 | HeLa | hTNF-α or IL-1β (20 ng/ml), both with Calyculin A (100 nM) for 10 min | |
| Phospho-TAK1 (Thr184/187) Antibody #4531 | HeLa | hTNF-α or IL-1β (20 ng/ml), both with Calyculin A (100 nM) for 10 min | |
| Phospho-TAK1 (Thr187) Antibody #4536 | HeLa | hTNF-α or IL-1β (20 ng/ml), both with Calyculin A (100 nM) for 10 min | |
| Phospho-TAK1 (Ser412) Antibody #9339 | K-562 | Untreated | |
| Phospho-Talin (Ser425) Antibody #5426 | 293 | Untreated | |
| Phospho-Tau (Ser396) (PHF13) Mouse mAb #9632 | Mouse brain, rat brain | Untreated | |
| Phospho-TBC1D1 (Thr590) Antibody #6927 | Transfected† | Transfect with construct overexpressing TBC1D1 | |
| Phospho-TBC1D1 (Ser660) Antibody #6928 | Transfected† | Transfect with construct overexpressing TBC1D1 | |
| Phospho-TBC1D1 (Ser700) Antibody #6929 | Transfected† | Transfect with construct overexpressing TBC1D1 | |
| Phospho-TCTP (Ser46) Antibody #5251 | HT-29 | 2 mM Thymidine for 16 hrs, then wash plate, add fresh media and 100 ng/ml Nocodazole O/N | |
| Phospho-Threonine (42H4) Mouse mAb #9386 | NIH/3T3 | PDGF-BB (100 ng/ml for 5 min) | |
| Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381 | NIH/3T3 | PDGF-BB (100 ng/ml for 5 min) | |
| Phospho-Threonine Antibody (P-Thr-Polyclonal) (HRP Conjugate) #6949 | NIH/3T3 | PDGF-BB (100 ng/ml for 5 min) | |
| Phospho-Tie2 (Tyr992) Antibody #4221 | Transfected† | 293 cells transfected with wild-type Tie2 | |
| Phospho-Tie2 (Ser1119) Antibody #4226 | Transfected† | Sf9 cells overexpressing GST-human Tie2 kinase domain fusion protein | |
| Phospho-TIF1β (Ser824) Antibody #4127 | HeLa | IR (10 Gy), 1 hr recovery | |
| Phospho-TLK1 (Ser695) Antibody #4121 | SK-N-MC | Serum-starve overnight | |
| Phospho-TNK1 (Tyr277) Antibody #4553 | L-540 | untreated (L-540 cells express a 58 kDa TNK1-C17orf61 fusion protein containing 466 amino acids from the amino terminus of TNK1) | |
| Phospho-TNK1 (Tyr277) (D46E7) Rabbit mAb #5638 | L-540 | Untreated (L-540 cells express a 58 kDa TNK1-C17orf61 fusion protein containing 466 amino acids from the amino terminus of TNK1) | |
| Phospho-Torc1/Crtc1 (Ser151) Antibody #3359 | Transfected† | Transfect with construct overexpressing Torc1/Crtc1 | |
| Phospho-Tpl2 (Ser400) Antibody #4491 | Transfected† | Transfect with a construct overexpressing Tpl2 | |
| Phospho-TrkA (Tyr490)/TrkB (Tyr516) (C35G9) Rabbit mAb #4619 | PC-12 | NGF (100 ng/ml for 2–5 min) | |
| Phospho-TrkA (Tyr490) Antibody #9141 | PC-12 | NGF (100 ng/ml for 2–5 min) | |
| Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621 | PC-12 | NGF (100 ng/ml for 2–5 min) | |
| Phospho-TrkA (Tyr785)/TrkB (Tyr816) (C67C8) Rabbit mAb #4168 | PC-12 | NGF (100 ng/ml for 2–5 min) | |
| Phospho-Troponin I (Cardiac) (Ser23/24) Antibody #4004 | Primary cardiac myocytes | Isoproterenol (1 µM for 5 min) | |
| Phospho-Tuberin/TSC2 (Ser939) Antibody #3615 | NIH/3T3 | PDGF (50 ng/ml for 30 min) | |
| Phospho-Tuberin/TSC2 (Ser1254) Antibody #3616 | MCF-7 | Untreated | |
| Phospho-Tuberin/TSC2 (Ser1387) Antibody #5584 | SHY5Y | Untreated | |
| Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb #3617 | MCF-7 | hEGF (10 ng/ml for 30 min) | |
| Phospho-Tuberin/TSC2 (Thr1462) Antibody #3611 | MCF-7 | hEGF (10 ng/ml for 30 min) | |
| Phospho-Tuberin/TSC2 (Tyr1571) Antibody #3614 | NIH/3T3 | PDGF (50 ng/ml for 30 min) | |
| Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb #5335 | HeLa | TSA (400 nM for 16 hrs) | |
| Acetyl-α-Tubulin (Lys40) Antibody #3971 | HeLa | TSA (400 nM for 16 hrs) | |
| Phospho-Tyk2 (Tyr1054/1055) Antibody #9321 | HeLa | Serum-starve overnight, IFN-α (100 ng/ml for 15 min) | |
| Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791 | HeLa | TPA (200 nM for 30 min) | MEK1/2 HeLa Control Cell Extracts #9160 |
| Phospho-Tyrosine Hydroxylase (Ser31) Antibody #3370 | Rat brain, mouse brain, human brain, SHSY5Y, Neuro2A, PC12 | Untreated | |
| Phospho-ULK1 (Ser317) Antibody #6887 | Transfected† | CIP (500 units/ml for 2 hrs at 37°C) | |
| Phospho-ULK1 (Ser467) Antibody #4634 | Transfected† | CIP (500 units/ml for 2 hrs at 37°C) followed by λ-phosphatase (20,000 units/ml for 2 hrs at 30°C) | |
| Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb #5869 | MCF7 | Oligomycin (0.5 μM for 30 minutes) | |
| Phospho-ULK1 (Ser757) Antibody #6888 | A-431 | Serum-starve overnight, hEGF (100 ng/ml for 30 min) | |
| Phospho-VASP (Ser157) Antibody #3111 | A-431 | Serum-starve overnight, Forskolin (10 µM for 30 min) | |
| Phospho-VASP (Ser239) Antibody #3114 | A-431 | Serum-starve overnight, Forskolin (10 µM for 30 min) | |
| Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb #4991 | Transfected† | CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains | VEGF Receptor 2 Control Proteins #2904 |
| Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb #2476 | Transfected† | CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains | VEGF Receptor 2 Control Proteins #2904 |
| Phospho-VEGF Receptor 2 (Tyr951) Antibody #2471 | Transfected† | CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains | VEGF Receptor 2 Control Proteins #2904 |
| Phospho-VEGF Receptor 2 (Tyr996) Antibody #2474 | Transfected† | CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains | VEGF Receptor 2 Control Proteins #2904 |
| Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb #2478 | HUVEC | Serum-starve overnight, VEGF (100 ng/ml for 5 min) | VEGF Receptor 2 Control Proteins #2904 |
| Phospho-VEGF Receptor 2 (Tyr1175) (D5B11) Rabbit mAb #3770 | HUVEC | Serum-starve overnight, VEGF (100 ng/ml for 5 min) | VEGF Receptor 2 Control Proteins #2904 |
| Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb #2477 | Transfected† | CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains | VEGF Receptor 2 Control Proteins #2904 |
| Phospho-VEGF Receptor 2 (Tyr1059) (D5A6) Rabbit mAb #3817 | Transfected† | CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGFR-2 intracellular domains | VEGF Receptor 2 Control Proteins #2904 |
| Phospho-Vimentin (Ser56) Antibody #3877 | HeLa | Paclitaxel (100 ng/ml for 20 hrs) | |
| Phospho-Vimentin (Ser82) Antibody #3878 | C2C12, C6 | Untreated | |
| Phospho-Wee1 (Ser642) (D47G5) Rabbit mAb #4910 | A-431 | serum-starve overnight, hEGF (100 nM for 5 min) | |
| Phospho-WNK1 (Thr60) Antibody #4946 | HT-29 | Serum-starve overnight, hIGF-I (100 ng/ml for 30 min) | |
| Phospho-YAP (Ser127) Antibody #4911 | HeLa | Untreated | |
| Phospho-YB1 (Ser102) (C34A2) Rabbit mAb #2900 | MCF-7 | Serum-starve overnight, hIGF-I (100 ng/ml for 30 min) | |
| Phospho-Zap-70 (Tyr493) Antibody #2704 | Jurkat | Serum-starve overnight, H2O2 (2 mM for 2 min) | |
| Phospho-Zyxin (Ser142/143) (D1E8) Rabbit mAb #8467 | A-431 | Untreated | |
| Phospho-Zyxin (Ser142/143) Antibody #4863 | A-431 | hEGF (100 ng/ml for 30 min) | |
| Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb #2599 | Any cell line | Transfected protein FoxO1, FoxO3a or FoxO4 | |
| Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb #2717 | Jurkat | Serum-starve overnight, H2O2 (2 mM for 2 min) | |
| Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody #2701 | Jurkat | Serum-starve overnight, H2O2 (2 mM for 2 min) | |
