SimpleChIP™ Control PCR Primers

SimpleChIP™ Control PCR Primersは2種類のコントロールプライマーのミックスで、ChIPアッセイで得られるDNAの増幅にご使用いただけます。これらのプライマーは、ChIPアッセイで使用した抗体で検出した標的タンパク質の既知の結合サイトを含む、ポジティブコントロールDNA配列を増幅します。また、抗体の感度を確認するために、ネガティブコントロールとしてもご使用いただけます。

プライマーはCST社内で設計し、CST社のChIP用抗体とSimpleChIP™ Kitを用いた規格試験で最適化しましたので、貴重な時間と試薬の無駄を省けます。
プライマーは、SYBR^® Green dyeを用いたリアルタイムPCR用に最適化されています。
テクニカルサポートは、製品を設計、製造している製品開発者が担当いたします。

SimpleChIP™ Human GAPDH Promoter Primers #4471

DNA isolated from cross-linked cells using the SimpleChIP™ Enzymatic Chromatin IP Kit (Magnetic Beads) #9003.

Figure 1. Amplification - SimpleChIP™ Human GAPDH Promoter Primers #4471 were tested on DNA isolated from cross-linked cells using the SimpleChIP™ Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR^® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R²) were calculated based on the corresponding threshold cycle (CT) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).

PCR product melting curves using SimpleChIP™ Human GAPDH Promoter Primers #4471.

Figure 2. Specificity - PCR product melting curves were obtained on real-time PCR reactions performed using SimpleChIP™ Human GAPDH Promoter Primers #4471. Data is shown for both duplicate PCR reactions using 20 ng of total DNA. The melt curve consists of 80 melt cycles, starting at 55°C with increments of 0.5°C per cycle. Each peak is formed from the degradation of a single PCR product.

SimpleChIP™ Control PCR Primers

CSTコード	品名	適用	種交差性
CSTコード	品名	適用	種交差性
4471	SimpleChIP™ Human GAPDH Promoter Primers	ChIP	H
4478	SimpleChIP™ Human GAPDH Intron 2 Primers	ChIP	H
4486	SimpleChIP™ Human α Satellite Repeat Primers	ChIP	H
4490	SimpleChIP™ Human MyoD1 Exon 1 Primers	ChIP	H
4493	SimpleChIP™ Human MYT-1 Exon 1 Primers	ChIP	H
4641	SimpleChIP™ Human Oct-4 Promoter Primers	ChIP	H
4649	SimpleChIP™ Human Sox2 Promoter Primers	ChIP	H
4653	SimpleChIP™ Mouse Oct-4 Promoter Primers	ChIP	M
4659	SimpleChIP™ Mouse XIST Intron 1 Primers	ChIP	M
4663	SimpleChIP™ Human c-Fos Promoter Primers	ChIP	H
4669	SimpleChIP™ Human CDKN1A Intron 1 Primers	ChIP	H
4779	SimpleChIP™ Human NPM1 Intron 1 Primers	ChIP	H
4829	SimpleChIP™ Human NR4A3 Promoter Primers	ChIP	H
5037	SimpleChIP™ Human γ-Actin Promoter Primers	ChIP	H
5047	SimpleChIP™ Human γ-Actin Intron 3 Primers	ChIP	H
5077	SimpleChIP™ Human Sat2 Repeat Element Primers	ChIP	H
5098	SimpleChIP™ Human AFM Intron 1 Primers	ChIP	H
5111	SimpleChIP™ Human CaMK2D Intron 3 Primers	ChIP	H
5131	SimpleChIP™ Mouse CIS Intron 1 Primers	ChIP	M
5139	SimpleChIP™ Human ID1 Promoter Primers	ChIP	H
5148	SimpleChIP™ Human TAP1 Promoter Primers	ChIP	H
5156	SimpleChIP™ Human SUB1 Promoter Primers	ChIP	H
5172	SimpleChIP™ Human H19/Igf2 Imprinting Control Region Primers	ChIP	H
5516	SimpleChIP™ Human GAPDH Exon 1 Primers	ChIP	H
5517	SimpleChIP™ Human HoxA2 Promoter Primers	ChIP	H
7014	SimpleChIP™ Human RPL30 Exon 3 Primers	ChIP	H
7015	SimpleChIP™ Mouse RPL30 Intron 2 Primers	ChIP	M

Reactivity Key: H=Human M=Mouse