サイトカイン & 成長因子 – 一貫性
サイトカイン製品の一貫性
製品を信頼していただくために:
- ロット毎に純度と生物活性を過去ロットと比較し、一貫性を確認しています。
- 厳密な規格を定め、それに則って規格試験を実施しています。
- 凍結乾燥品は、凍結乾燥前後に無菌試験と生物活性試験を実施しています。
Lot-to-Lot Consistency
Comparison of purity and bioactivity of three independent lots of Human Interleukin-6 (hIL-6) #8904:
The purity of recombinant IL-6 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant IL-6 and staining overnight with Coomassie Blue.
TF-1 is a pre-myeloid cell line orginally derived from a patient with erythroleukemia. These cells are often used for bioassays of various cytokines including IL-6, which induces proliferation of TF-1 cells. Here, the proliferation of TF-1 cells treated with increasing concentrations of IL-6 was assessed after 48 hour treatment with IL-6, cells were incubated with a tetrazolium salt and the OD450–OD650 was determined.