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免疫蛍光染色 (IF) 用Alexa Fluor®標識抗体

蛍光標識抗体は、IFでの使用に有用です。なぜなら、単一サンプル中の標的タンパク質を特定する目的で、抗体の由来種に関係なく異なる蛍光色素で標識された複数の一次抗体を用いたマルチプレックスアッセイが可能となり、アッセイの柔軟性が格段に高くなるからです。CSTのすべての蛍光標識抗体は、S/N比を最大化するために最適な標識密度が検討されており、IFでの使用が厳密に検証され、最適な抗体希釈率とプロトコールが定められています。

Cellular Dyes

XP®CodeUnconjugated ProductAlexa 488Alexa 555Alexa 594Alexa 647
Phalloidin887889538940

Companion Products

XP®CodeUnconjugated ProductAlexa 488Alexa 555Alexa 594Alexa 647
5415Mouse (G3A1) mAb IgG1 Isotype Control96418527
XP3900Rabbit (DA1E) mAb IgG XP® Isotype Control297539692985
Anti-Mouse IgG (H+L), F(ab')2 Fragment4408440988904410
Anti-Rabbit IgG (H+L), F(ab')2 Fragment4412441388894414
Anti-Rat IgG (H+L)441644174418

Epitope Tag Antibodies

XP®CodeUnconjugated AntibodyReactivityAlexa 488Alexa 555Alexa 594Alexa 647
XP®CodeUnconjugated AntibodyReactivityAlexa 488Alexa 555Alexa 594Alexa 647
2368DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody)540796963916
2624GST (26H1) Mouse mAbAll3368
2367HA-Tag (6E2) Mouse mAb23503444
XP12698His-Tag (D3I1O) XP® Rabbit mAbAll1493014931
2276Myc-Tag (9B11) Mouse mAbAll2279375694832233

Target-Specific Antibodies

XP®CodeUnconjugated AntibodyReactivityAlexa 488Alexa 555Alexa 594Alexa 647
XP®CodeUnconjugated AntibodyReactivityAlexa 488Alexa 555Alexa 594Alexa 647
4970β-Actin (13E5) Rabbit mAbH M R Mk B Pg (Hm C Dg Hr)8844804694708584
XP8243β-Amyloid (D54D2) XP® Rabbit mAbH51374
4691Akt (pan) (C67E7) Rabbit mAbH M R Mk Dm (Pg)5186
XP13038Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAbH M R Mk43506
XP5153Androgen Receptor (D6F11) XP® Rabbit mAbH7395895689387397
XP93668Arginase-1 (D4E3M™) XP® Rabbit mAbH M R66297
67824ASC (D2W8U) Rabbit mAb (Mouse Specific)M17507
XP2914Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (D13A11) XP® Rabbit mAbH M R852513464
14472E-Cadherin (4A2) Mouse mAbH M R86770
3195E-Cadherin (24E10) Rabbit mAbH M (B Dg Pg)31997687
9579Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAbH M (R B Pg)9603960481729602
9661Cleaved Caspase-3 (Asp175) AntibodyH M R Mk (B Dg Pg)9669
2677β-Catenin (L54E2) Mouse mAb (IF Preferred)H (M R Pg)284956124627
XP19807Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAbH M R Mk Z Dg70034
14697Cas9 (7A9-3A3) Mouse mAbAll3496348796
14732Phospho-CD79A (Tyr182) (D1B9) Rabbit mAbH (M)5282129742
4850COX IV (3E11) Rabbit mAbH R Mk Z B Pg4853869386927561
9198Phospho-CREB (Ser133) (87G3) Rabbit mAbH M R (Z)918714001
14472E-Cadherin (4A2) Mouse mAbH M R86770
3195E-Cadherin (24E10) Rabbit mAbH M (B Dg Pg)31997687
XP4267EGF Receptor (D38B1) XP® Rabbit mAbH M Mk5616510887425588
2929EpCAM (VU1D9) Mouse mAbH5198548873195447
2118GAPDH (14C10) Rabbit mAbH M R Mk B Pg (Pg)39063907
3670GFAP (GA5) Mouse mAbH M R3655365681523657
XP3660Glucocorticoid Receptor (D8H2) XP® Rabbit mAbH M R Mk12007
9718Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAbH M R Mk9720
XP4499Histone H3 (D1H2) XP® Rabbit mAbH M R Mk (Hm C Dm X Z B)12230
9751Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAbH M R Mk Dm Sc (X Z)1196012064
9649Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAbH M R Mk Z (Sc)96834484
27683Acetyl-Histone H3 (Lys36) (D9T5Q) Rabbit mAbH M R Mk (Hm Dm X Z Pg)42599
13534Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAbH M R Mk56999
XP3377Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAbH M R Mk Z3465347584813458
9701Phospho-Histone H3 (Ser10) AntibodyH M R Mk Dm Sc (X)9716
XP9728Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAbH M R Mk12244
9733Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAbH M R Mk (X Z)549912158
XP8714Phospho-HS1 (Tyr397) (D12C1) XP® Rabbit mAbH (M R)13708
13120iNOS (D6B6S) Rabbit mAbM93421
3014Insulin (C27C9) Rabbit mAbH M R90169008
4545Pan-Keratin (C11) Mouse mAbH M R Mk452334784528
9129Ki-67 (D3B5) Rabbit mAbH M R1188212075
3308c-Kit (Ab81) Mouse mAbH3310
4777Lamin A/C (4C11) Mouse mAbH M R Mk8617
XP12741LC3A/B (D3U4C) XP® Rabbit mAbH M R (X B Dg Pg)13082131731407913394
XP8641LIN28A (D1A1A) XP® Rabbit mAbH M (R Mk)12573
XP8198Met (D1C2) XP® Rabbit mAbH8494
XP4903Nanog (D73G4) XP® Rabbit mAbH87505448
XP5482Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAbH M R Mk69927497
XP24307NeuN (D4G4O) XP® Rabbit mAbH M R54761
2837Neurofilament-L (C28E10) Rabbit mAbH M R80248743
XP8242NF-κB p65 (D14E12) XP® Rabbit mAbH M R Hm Mk Dg494458801
2598NUP98 (C39A3) Rabbit mAbH M R Mk13393
2840Oct-4A (C30A3) Rabbit mAbH M517744395263
2947p21 Waf1/Cip1 (12D1) Rabbit mAbH Mk (Dg)54878493118508587
XP4511Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAbH M R Mk Mi Pg Sc (Hm C Z B Pg)8632
2527p53 (7F5) Rabbit mAbH Mk5429
XP5625Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAbH Mk914868946987
2586PCNA (PC10) Mouse mAbH M R Mk B Pg8580
XP5241PDGF Receptor α (D13C6) XP® Rabbit mAbH88718893
3501PDI (C81H6) Rabbit mAbH M R Mk50518615
86744PD-L1 (Extracellular Domain Specific) (D8T4X) Rabbit mAbH25048402165994941726
XP8516Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAbH M R Mk42778957
2317S6 Ribosomal Protein (54D2) Mouse mAbH M R Mk Dm53175548
XP4858Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAbH M R Mk Mi Sc (C Pg)4803398598654851
4856Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAbH M R Mk4854
XP5364Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAbH M R Mk (C Pg)50189468
XP3579Sox2 (D6D9) XP® Rabbit mAbH (B Dg Hr)504951795067
82630Sox9 (D8G8H) Rabbit mAbH (M R)9479471273
88588SQSTM1/p62 (D5L7G) Mouse mAbH66277
9167Phospho-Stat1 (Tyr701) (58D6) Rabbit mAbH M8183
12640Stat3 (D3Z2G) Rabbit mAbH M R Mk (B Pg)14047
2808Survivin (71G4B7) Rabbit mAbH M R2810
2710Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAbH (M R)434912081
XP5297Synapsin-1 (D12G5) XP® Rabbit mAbH M R1319711127
XP4179α-Synuclein (D37A6) XP® Rabbit mAbM R3809
XP13232T-bet/TBX21 (D6N8B) XP® Rabbit mAbH1429814307
2125α-Tubulin (11H10) Rabbit mAbH M R Mk Dm Z B Pg (Dg)506350595046
3873α-Tubulin (DM1A) Mouse mAbH M R Mk8058
XP5335Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAbH M R Mk Z (X)81502
2128β-Tubulin (9F3) Rabbit mAbH M R Mk Z B (C)3623211676343624
9411Phospho-Tyrosine Mouse mAb (P-Tyr-100)H M R All9415
XP5741Vimentin (D21H3) XP® Rabbit mAbH M R Mk9854985576759856

免疫蛍光染色 (IF) 用Alexa Fluor®標識抗体

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