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Protein Substrates

Cell Signaling Technology (CST) offers a variety of protein kinase substrates useful for detecting the activity of protein kinases involved in cell cycle and signal transduction pathways. All of the substrates are cloned and overexpressed in E. coli and purified by affinity chromatography to assure the absence of contaminating eukaryotic kinases and phosphatases.

Quality Assurance

Protein kinase substrates are examined for purity by SDS-PAGE analysis and quality control tested by Western blotting using our complementary antibodies. Each substrate is also tested for its ability to be phosphorylated by its upstream kinase. This is confirmed by Western blotting with the appropriate CST phospho-specific antibody.

Protein Kinase Substrates Residues Kinase Phosphorylation Sites MW
9224 ATF-2 Fusion Protein 19–96 p38 MAPK, SAPK/JNK Thr69, Thr71 34 kDa
9184 Elk-1 Fusion Protein 307–428 MAPK/Erk, SAPK/JNK Ser383 41 kDa
9237 GSK-3 Fusion Protein 18–24 Akt1 Ser21 27 kDa
6093 c-Jun Fusion Protein 1–89 SAPK/JNK Ser63, Ser73 37 kDa
9811 c-Jun Fusion Protein Beads 1–89 SAPK/JNK Ser63, Ser73
Pathways & Diagrams for Epigenetic Regulation