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#10831 HDAC7 (E7O8V) Rabbit mAb

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2019年3月22日15時35分 現在
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#10831S100 μL56,000

HDAC7 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性124Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


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10831 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:100)免疫蛍光細胞染色 (IF-IC) (1:100)


HDAC7 (E7O8V) Rabbit mAb recognizes endogenous levels of total HDAC7 protein. This antibody does not cross-react with other HDAC proteins, including HDAC4 and HDAC5.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly60 of human HDAC7 protein.

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※下記の社内データは、すべて10831 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC7 (E7O8V) Rabbit mAb.



Immunoprecipitation of HDAC7 from HCT 116 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HDAC7 (E7O8V) Rabbit mAb. Western blot analysis was performed using HDAC7 (E7O8V) Rabbit mAb.



Confocal immunofluorescent analysis of HUVEC cells, untreated (left) or treated with TPA #4174 (10 ng/mL, 4 hr; right) to promote cytoplasmic accumulation, using HDAC7 (E7O8V) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Cells were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).


Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

15164   HDAC4 (D8T3Q) Rabbit mAb
20458   HDAC5 (D1J7V) Rabbit mAb
33418   HDAC7 (D4E1L) Rabbit mAb
3424   Phospho-HDAC4 (Ser632)/HDAC5 (Ser661)/HDAC7 (Ser486) Antibody
34589   HDAC1 (D5C6U) XP® Rabbit mAb
3815   Phospho-HDAC3 (Ser424) Antibody
57156   HDAC2 (D6S5P) Rabbit mAb
65816   Class I HDAC Antibody Sampler Kit
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7558   HDAC6 (D2E5) Rabbit mAb
7612   HDAC6 (D21B10) Rabbit mAb
7727   Biotinylated Protein Ladder Detection Pack
79891   Class II HDAC Antibody Sampler Kit
85057   HDAC3 (D2O1K) Rabbit mAb
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
ProLong is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.


HDAC7 (E7O8V) Rabbit mAb

Metabolic Reprogramming in Disease