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#12666 Endosomal Marker Antibody Sampler Kit

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希望納入価格 (円)
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2019年3月20日11時40分 現在
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#12666T1 Kit92,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Caveolin-1 (D46G3) XP® Rabbit mAb #3267 20 µl WB, IP, IHC-P, IF-IC, F H, M, R, Hm, Mk, B, Dg 21, 24 Rabbit IgG
Clathrin Heavy Chain (D3C6) XP® Rabbit mAb #4796 20 µl WB, IP, IF-IC H, M, R, Mk Pg 190 Rabbit IgG
EEA1 (C45B10) Rabbit mAb #3288 20 µl WB, IP, IF-IC H, M, R 170 Rabbit IgG
Rab5 (C8B1) Rabbit mAb #3547 20 µl WB, IF-IC H, M, R, Mk 25 Rabbit
Rab7 (D95F2) XP® Rabbit mAb #9367 20 µl WB, IP, IF-IC H, M, R, Mk 23 Rabbit IgG
Rab11 (D4F5) XP® Rabbit mAb #5589 20 µl WB, IP, IF-IC H, M, R, Mk Hm, Pg 25 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Hm=Hamster, Mk=Monkey, B=Bovine, Dg=Dog

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Caveolin-1 (D46G3) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using EEA1 (C45B10) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Rab5 (C8B1) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Rab7 (D95F2) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Clathrin Heavy Chain (D3C6) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Rab11 (D4F5) XP® Rabbit mAb.

IP

IP

Immunoprecipitation of caveolin-1 from HeLa cells using Caveolin-1 (D46G3) XP® Rabbit mAb followed by western blot using the same antibody. Lane 1 is 5% input.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using EEA1 (C45B10) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Rab5 (C8B1) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-MEL-28 cells using Rab7 (D95F2) XP® Rabbit mAb (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of SH-SY5Y cells using Clathrin Heavy Chain (D3C6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IF-IC

IF-IC

Confocal immunofluorescent analysis of A549 cells using Rab11 (D4F5) XP® Rabbit mAb (green). Actin filaments were labeled wtih DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Caveolin-1 (D46G3) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using Caveolin-1 (D46G3) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using Caveolin-1 (D46G3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Hela cells using Caveolin-1 (D46G3) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of C2C12 cells using Caveolin-1 (D46G3) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).


バックグラウンド

Endosomes are formed by the invagination of the plasma membrane to form vesicles in an effort to recycle components of the cell (1). Endosomes can be coated in clathrin when vesicles form at clathrin-coated pits (2). Caveolins are 21-24 kDa integral proteins that interact with cholesterol and are the main structural components of the cholesterol/sphingolipid-enriched plasma membrane caveolae (3). Each stage of endosome maturation is marked by a unique set of proteins. EEA1 is an early endosome marker that is essential for membrane fusion and trafficking (4). Members of the ras superfamily of small Rab GTPases, specifically Rab5, Rab7, and Rab11 are markers of the early, late and recycling endosomes (5).

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DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Endosomal Marker Antibody Sampler Kit

Metabolic Reprogramming in Disease

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