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#12866 Glycolysis II Antibody Sampler Kit

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希望納入価格 (円)
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2019年1月17日15時10分 現在
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Glycolysis 製品一覧

12866 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Aldolase A (D73H4) Rabbit mAb #8060 20 µl WB H, M, Mk 40 Rabbit IgG
Enolase-1 Antibody #3810 20 µl WB, IP H, M, R, Mk 47 Rabbit
Enolase-2 (D20H2) Rabbit mAb #8171 20 µl WB, IP H, M, R, Mk 47 Rabbit IgG
PDHK1 (C47H1) Rabbit mAb #3820 20 µl WB, IP H, M, R, Mk B 47 Rabbit IgG
PFKFB2 (D7G5R) Rabbit mAb #13045 20 µl WB, IP, IF-IC H, Mk 55 Rabbit IgG
PFKFB3 (D7H4Q) Rabbit mAb #13123 20 µl WB H, M, R, Mk 60 Rabbit IgG
PGAM1 (D3J9T) Rabbit mAb #12098 20 µl WB H, M, R, Hm, Mk 28 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human, M=Mouse, Mk=Monkey, R=Rat, Hm=Hamster

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Enolase-1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using PDHK1 (C47H1) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Enolase-2 (D20H2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Aldolase A (D73H4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PGAM1 (D3J9T) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PFKFB3 (D7H4Q) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from LNCaP, Hep G2, and MCF7 cells using PFKFB2 (D7G5R) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human PFKFB3 (hPFKFB3-DDK; +), using PFKFB3 (D7H4Q) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).


IP

IP

Immunoprecipitation of PFKFB2 from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PFKFB2 (D7G5R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PFKFB2 (D7G5R) Rabbit mAb. An anti-rabbit IgG light chain antibody was used as the secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of LNCaP (high expression, left) and Hep G2 (low expression, right) cells using PFKFB2 (D7G5R) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level. Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (2). Aldolase (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde (3). Phosphoglycerate mutase (PGAM1) catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis (4). Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers (5). Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form (6).

使用例
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9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Glycolysis II Antibody Sampler Kit

Immune Cell Signaling Pathways

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製品特集

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