#12866 Glycolysis II Antibody Sampler Kit
|Aldolase A (D73H4) Rabbit mAb #8060||20 µl||WB||H, M, Mk||40||Rabbit IgG|
|Enolase-1 Antibody #3810||20 µl||WB, IP||H, M, R, Mk||47||Rabbit|
|Enolase-2 (D20H2) Rabbit mAb #8171||20 µl||WB, IP||H, M, R, Mk||47||Rabbit IgG|
|PDHK1 (C47H1) Rabbit mAb #3820||20 µl||WB, IP||H, M, R, Mk||B||47||Rabbit IgG|
|PFKFB2 (D7G5R) Rabbit mAb #13045||20 µl||WB, IP, IF-IC||H, Mk||55||Rabbit IgG|
|PFKFB3 (D7H4Q) Rabbit mAb #13123||20 µl||WB||H, M, R, Mk||60||Rabbit IgG|
|PGAM1 (D3J9T) Rabbit mAb #12098||20 µl||WB||H, M, R, Hm, Mk||28||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||WB||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human, M=Mouse, Mk=Monkey, R=Rat, Hm=Hamster
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from various cell types using Enolase-1 Antibody.
Western blot analysis of extracts from various cell types using PDHK1 (C47H1) Rabbit mAb.
Western blot analysis of extracts from various cell lines using Enolase-2 (D20H2) Rabbit mAb.
Western blot analysis of extracts from various cell lines using Aldolase A (D73H4) Rabbit mAb.
Western blot analysis of extracts from various cell lines using PGAM1 (D3J9T) Rabbit mAb.
Western blot analysis of extracts from various cell lines using PFKFB3 (D7H4Q) Rabbit mAb.
Western blot analysis of extracts from LNCaP, Hep G2, and MCF7 cells using PFKFB2 (D7G5R) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human PFKFB3 (hPFKFB3-DDK; +), using PFKFB3 (D7H4Q) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of PFKFB2 from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or PFKFB2 (D7G5R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using PFKFB2 (D7G5R) Rabbit mAb. An anti-rabbit IgG light chain antibody was used as the secondary antibody.
Confocal immunofluorescent analysis of LNCaP (high expression, left) and Hep G2 (low expression, right) cells using PFKFB2 (D7G5R) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Glycolysis is the metabolic process by which glucose is converted to pyruvate in a sequence of enzymatic steps. Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate in glycolysis (1). The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level. Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (2). Aldolase (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde (3). Phosphoglycerate mutase (PGAM1) catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis (4). Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers (5). Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form (6).
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DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.