Confocal immunofluorescent analysis of HeLa cells using Cox IV (4D11-B3-E8) Mouse mAb #11967 (red). Actin filaments were labeled with DyLight™ 488 Phalloidin (green). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).
Actin is a ubiquitous eukaryotic protein and a major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells to control cell structure and motility (1). Actin exists mainly as a fibrous polymer called F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin resulting in an increase in the monomeric globular G-actin form (2). Phalloidin is a naturally occurring toxic bicyclic peptide found in the deathcap toadstool, Amanita phalloides, which rapidly binds to F-actin with strong affinity (3).
DRAQ5 is a registered trademark of Biostatus Limited.
ProLong is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.