#13430 Focal Adhesion Protein Antibody Sampler Kit
|α-Actinin (D6F6) XP® Rabbit mAb #6487||20 µl||WB, IF-IC||H, M, R, Mk||Z||100||Rabbit IgG|
|FAK Antibody #3285||20 µl||WB, IP, IHC-P||H, M, R, Mk, B, Pg||C||125||Rabbit|
|Paxillin (D9G12) Rabbit mAb #12065||20 µl||WB, IP||H||54, 62, 68||Rabbit IgG|
|Talin-1 (C45F1) Rabbit mAb #4021||20 µl||WB, IHC-P||H, M, R, Hm, Mk||270||Rabbit IgG|
|Tensin 2 Antibody #11990||20 µl||WB, IP||H, M, R||145-155||Rabbit|
|Vinculin Antibody #4650||20 µl||WB||H, M, R, Mk, Dg||124||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||WB||Goat|
†Species predicted to react based on 100% sequence homology.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey, B=Bovine, Pg=Pig, Hm=Hamster, Dg=Dog
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from various cell types using Talin-1 (C45F1) Rabbit mAb.
Western blot analysis of extracts from various cell and tissue types using Vinculin Antibody.
Western blot analysis of extracts from various cell lines using α-Actinin (D6F6) XP® Rabbit mAb.
Western blot analysis of extracts from various cell lines using Paxillin (D9G12) Rabbit mAb. The 45 kDa band is a degradation product of full-length paxillin protein.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using FAK Antibody.
Confocal immunofluorescent analysis of SNB19 cells using α-Actinin (D6F6) XP® Rabbit mAb (green) showing colocalization with actin filaments that were labeled with β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Talin-1 (C45F1) Rabbit mAb.
Focal adhesions connect the cytoskeleton with the extracellular matrix (ECM), a complex structure of secreted macromolecules that surrounds mammalian organs and tissues. Integrins clustered on the extracellular side of focal adhesions relay signals from the ECM to intracellular protein complexes that signal the actin cytoskeleton to regulate tension for cell motility. Internal signals converge on focal adhesions to regulate integrin receptor affinity and avidity. Signaling through focal adhesions regulates cell adhesion, migration, proliferation, apoptosis, and gene expression, and impacts cellular processes such as development, wound healing, immune response, invasion, metastasis and angiogenesis (reviewed in 1-3). Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. Integrin clustering triggers FAK recruitment to the focal adhesion complex (4). Talin is a large, multidomain focal adhesion protein that interacts with the intracellular domains of integrins and other focal adhesion proteins. Talin is involved in the formation of focal adhesions and in linking focal adhesions to the actin cytoskeleton (5). Paxillin is a key component of integrin signaling that localizes primarily to focal adhesion sites in the extracellular matrix (6). Tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (7). Paxillin is phosphorylated by FAK at Tyr118 (8,9). Vinculin is a cytoskeletal protein involved in regulation of focal adhesions and embryonic development (10-13). Active vinculin translocates to focal adhesions where it may be involved in anchoring F-actin to the membrane and regulating cell migration. Vinculin binds a number of proteins, including talin, α-actinin and paxillin (11,13). Tensin 2 localizes to focal adhesions of various tissues and exhibits highest expression in heart, kidney, and liver (14,15). Tensin 2 belongs to a family of cytoskeletal proteins that include Tensin 1-3 and Cten, which couple integrins to the actin cytoskeleton (16). Tensin family proteins play an important role in signal transduction, cell proliferation, and motility (17-20). α-actinin is a member of the spectrin family of cytoskeletal proteins that was first recognized as an actin cross-linking protein, but also interacts with a large number of cytoskeletal signaling proteins, including those involved in cellular adhesion, migration, and immune cell targeting (21).
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DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.