CST logo

#14205 Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2018年9月21日11時45分 現在
ご登録代理店情報 i
#14205S100 μL63,000

ULK1 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性140-150Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、サル -
14205 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)


内在性レベルのSer638 がリン酸化されたULK1 タンパク質を検出します。
ヒトのULK1 タンパク質のSer638 周辺領域 (合成リン酸化ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて14205 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from A172 cells, untreated (-), Torin 1-treated (250 nM; 5 hr; +), or INK128-treated (250 nM; 5 hr; +) using Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb (upper) or ULK1 (D8H5) Rabbit mAb #8054 (lower).

Western Blotting

Western Blotting

Western blot analysis of A172 cell extracts, untreated (-) or λ phosphatase and calf intestinal phosphatase (CIP)-treated (+), using Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb (upper) or ULK1 (D8H5) Rabbit mAb #8054 (lower).

Western Blotting

Western Blotting

Western blot analysis of various cell lines using Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb.


Two related serine/threonine kinases, UNC-51-like kinase 1 and 2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth (1-4). Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors, such as NGF (5). Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGAP and syntenin (6). Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1 (7). Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy (8), a catabolic process for the degradation of bulk cytoplasmic contents (9,10). It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy (11), and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking (12-16).

Phosphorylation of ULK1 at Ser638 and Ser757 is mediated by mTOR, which is a regulator of cell growth and an inhibitor of autophagy that disrupts the interaction between ULK1 and AMPK (17,18). Conversely, AMPK is activated during low nutrient conditions and directly phosphorylates ULK1 at multiple sites including Ser317, Ser555, and Ser777 (17-19).

14202   Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb
4773   ULK1 (R600) Antibody
4776   ULK1 (A705) Antibody
5869   Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb
6439   ULK1 (D9D7) Rabbit mAb
7000   SignalSilence® ULK1 siRNA I
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8054   ULK1 (D8H5) Rabbit mAb

Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb