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#14472 E-Cadherin (4A2) Mouse mAb

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CSTコード 包装
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2019年3月18日15時40分 現在
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#14472S100 μL58,000
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Cadherin E 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性135Mouse IgG1-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット -
14472 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:200)免疫組織染色 (パラフィン) (1:100)免疫蛍光細胞染色 (IF-IC) (1:50)フローサイトメトリー (1:200)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのE-Cadherin タンパク質を検出します。他のCadherin タンパク質とは交差しません。
使用抗原
ヒトのE-Cadherin タンパク質に特異的な組換えタンパク質

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社内データ

※下記の社内データは、すべて14472 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using E-Cadherin (4A2) Mouse mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 (+) and HeLa (-) cells using E-Cadherin (4A2) Mouse mAb (upper) and Pan-Cadherin (28E12) Rabbit mAb #4073 (lower).

IP

IP

Immunoprecipitation of E-cadherin from MCF7 cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or E-Cadherin (4A2) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot was performed using E-Cadherin (4A2) Mouse mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using E-Cadherin (4A2) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using E-Cadherin (4A2) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MCF7 (left) and HeLa (right) cell pellets using E-Cadherin (4A2) Mouse mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 (positive, left) and HeLa (negative, right) cells using E-Cadherin (4A2) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and MCF7 cells (green) using E-Cadherin (4A2) Mouse mAb. Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

バックグラウンド

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

使用例
 
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DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

E-Cadherin (4A2) Mouse mAb

Metabolic Reprogramming in Disease

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