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#14511 PhosphoPlus® Tau (Thr181) Antibody Duet

 
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年1月15日15時15分 現在
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#14511S1 Kit113,000
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Tau 製品一覧

14511 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 Mol. Wt. アイソタイプ
Phospho-Tau (Thr181) (D9F4G) Rabbit mAb #12885 100 µl WB, IP, IHC-P H, M, R 50-80 kDa Rabbit IgG
Tau (D1M9X) XP® Rabbit mAb #46687 100 µl WB, IHC-P, IF-F, IF-IC H, M, R 50 to 80 kDa Rabbit IgG

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry)

Reactivity Key: H=Human, M=Mouse, R=Rat

Kit 情報
Total 及び活性化特異的抗体が含まれ、ターゲットタンパク質の活性化状態の測定に最適です。CST抗体の中でお薦めする、最高品質の抗体をセットにしました。
貯法
-20℃
社内データ

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb. The phospho-specificity of Phospho-Tau (Thr181) (D9F4G) Rabbit mAb was verified by peptide blocking using a phosphopeptide or non-phosphopeptide targeting residue Thr181.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using Tau (D1M9X) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Alzheimer's brain using Tau (D1M9X) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human normal appendix using Tau (D1M9X) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded T-47D cell pellet (left, positive) or MDA-MB-231 cell pellet (right, negative) using Tau (D1M9X) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse colon, control (left) or λ phosphatase-treated (right), using Phospho-Tau (Thr181) (D9F4G) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using Tau (D1M9X) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of T-47D (positive, left) or MDA-MB-231 (negative, right) cells using Tau (D1M9X) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of mouse striatum (left), hippocampus (middle), or midbrain (right) using Tau (D1M9X) XP® Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


バックグラウンド

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

The cerebrospinal fluid concentration of Tau phosphorylated at Thr181 has been proposed to be a biomarker for the study of neurodegenerative disorders (4).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
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Tween is a registered trademark of ICI Americas, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
SignalStain is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

PhosphoPlus® Tau (Thr181) Antibody Duet

Metabolic Reprogramming in Disease

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