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#17336 One-Carbon Metabolism Antibody Sampler Kit

 
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希望納入価格 (円)
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2019年1月17日11時55分 現在
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#17336T1 Kit107,000
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キット内容 容量 用途 種交差性 検出分子量 アイソタイプ
AHCYL1/IRBIT (D3A5G) Rabbit mAb #94248 20 µl WB, IP H, M, R, Mk 61 Rabbit IgG
CBS (D8F2P) Rabbit mAb #14782 20 µl WB H, M, R 61 Rabbit IgG
Cystathionine γ-Lyase (D1N1D) Rabbit mAb #19689 20 µl WB H, M, R 44 Rabbit IgG
MTHFR (D1E4V) Rabbit mAb #25164 20 µl WB H 78 Rabbit IgG
 MTHFD1L (D8T7L) Rabbit mAb #14999 20 µl WB H, M 106 Rabbit IgG
MTHFD2 (D8W9U) Rabbit mAb #41377 20 µl WB, IP H 35 Rabbit IgG
SHMT1 (D3B3J) Rabbit mAb #80715 20 µl WB, IP H, M, R, Mk 50 Rabbit IgG
Thymidylate Synthase (D5B3) XP® Rabbit mAb #9045 20 µl WB, IHC-P, IF-IC, F H, M, R, Mk 30 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-BR-3 and HCC827 cells, untreated or treated with 5-FU (1 μM) or lapatinib (1 μM) for 24 hr, using Thymidylate Synthase (D5B3) XP® Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CBS (D8F2P) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MTHFD1L (D8T7L) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and 293 cells using MTHFD2 (D8W9U) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SHMT1 (D3B3J) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using AHCYL1/IRBIT (D3A5G) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from Daudi, Raji, and MOLT-4 cells using MTHFR (D1E4V) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from K-562 cells, mouse liver, and rat liver using Cystathionine γ-Lyase (D1N1D) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Thymidylate Synthase (D5B3) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb (lower) #8457.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Thymidylate Synthase (D5B3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using Thymidylate Synthase (D5B3) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse spleen using Thymidylate Synthase (D5B3) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded SK-BR-3 cell pellets, untreated (left) or treated with LY294002 #9901 (right), using Thymidylate Synthase (D5B3) XP® Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HCC827 cells (blue) and HeLa cells (green) using Thymidylate Synthase (D5B3) XP® Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of LN18 cells using Thymidylate Synthase (D5B3) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

One-carbon metabolism includes various enzymatic reactions involving the transfer of one-carbon groups mediated by folate cofactor (1). The activated one-carbon groups are used by various metabolic pathways, including purine synthesis, thymidine synthesis, and remethylation of homocysteine to methionine (1). S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1) is a member of the S-adenosylhomocysteine hydrolase family, which participates in the metabolism of S-adenosyl-L-homocysteine (2). Cystathionine beta-synthase (CBS) is a key enzyme involved in sulfur amino acid metabolism as it catalyzes the formation of cystathionine from serine and homocysteine (3,4). Cystathionine γ-lyase (CGL) is an enzyme in the transsulfuration pathway, a route in the metabolism of sulfur-containing amino acids (5). Methylenetetrahydrofolate reductase (MTHFR), a key enzyme in one-carbon metabolism, catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate (1). 5-methyltetrahydrofolate donates its methyl group for remethylation of homocysteine to methionine (1). Methionine is further converted to S-adenosylmethionine (SAM), a major reactive methyl carrier (1). NADP+ dependent methylenetetrahydrofolate dehydrogenase 1-like (MTHFD1L) is a mitochondrial enzyme that catalyzes the production of formate from 10-formyl-tetrahydrofolate in one-carbon flow from mitochondria to cytoplasm (6,7). MTHFD2 is a bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase involved in mitochondrial folate metabolism (8). Serine hydroxymethyltransferase 1 (SHMT1) is a cytoplasmic serine hydroxylmethyltransferase (9,10). It catalyzes the conversion of serine to glycine with the transfer of β-carbon from serine to tetrahydrofolate (THF) to form 5, 10-methylene-THF (9, 10). The methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) is an essential step in the formation of thymine nucleotides, a process catalyzed by thymidylate synthase (TS or TYMS) (11-13).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
14782   CBS (D8F2P) Rabbit mAb
14999    MTHFD1L (D8T7L) Rabbit mAb
19689   Cystathionine γ-Lyase (D1N1D) Rabbit mAb
25164   MTHFR (D1E4V) Rabbit mAb
41377   MTHFD2 (D8W9U) Rabbit mAb
80715   SHMT1 (D3B3J) Rabbit mAb
9045   Thymidylate Synthase (D5B3) XP® Rabbit mAb
94248   AHCYL1/IRBIT (D3A5G) Rabbit mAb

Tween is a registered trademark of ICI Americas, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
CST is a trademark of Cell Signaling Technology, Inc.
SignalFire is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

One-Carbon Metabolism Antibody Sampler Kit

Immune Cell Signaling Pathways

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