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#19495 Di-Methyl-RPL29 (Lys5) (D8T9P) Rabbit mAb

 

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2019年3月25日15時35分 現在
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#19495S100 μL66,000
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感度分子量 (kDa)抗体の由来貯法
内在性23Rabbit IgG-20℃
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特異性・感度
Di-Methyl-RPL29 (Lys5) (D8T9P) Rabbit mAb recognizes endogenous levels of RPL29 protein only when methylated at Lys5. This antibody shows slight cross-reactivity to mono-methyl-RPL29 Lys5.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic methyl peptide corresponding to residues surrounding Lys5 of human RPL29 protein.

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社内データ

※下記の社内データは、すべて19495 の推奨プロトコールで実験した結果です。

Dot Blot-Peptide

Dot Blot-Peptide

Peptide dot blot analysis demonstrating Di-Methyl-RPL29 (Lys5) (D8T9P) Rabbit mAb specificity. Antibody binding to pre-coated RPL29 peptides is shown using Di-Methyl-RPL29 (Lys5) (D8T9P) Rabbit mAb. As expected, the Di-Methyl-RPL29 (Lys5) (D8T9P) Rabbit mAb binds to RPL29 peptide when dimethylated at Lys5. The antibody also shows slight binding to RPL29 peptide that is monomethylated at Lys5.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, including NIH/3T3 RPL29 wild-type (WT) and NIH/3T3 RPL29 knockout (KO), using Di-Methyl-RPL29 (Lys5) (D8T9P) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

バックグラウンド

SET7/SET9 is a member of the SET domain-containing family, and can specifically methylate Lys4 on histone H3 (1). Like most other lysine-directed histone methyltransferases, it contains a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste and Trithorax proteins. Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Methylation of histone H3 Lys4 enhances transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (4,5). In addition, methylation of lysine 4 blocks transcriptional repression by inhibiting the binding of the NURD histone deacetylation complex to the amino-terminal tail of histone H3 and interfering with SUV39H1-mediated methylation of histone H3 Lys9 (1). SET7/SET9 is highly active on free histone H3, but only very weakly methylates H3 within nucleosomes (1). Besides histones, SET7/SET9 also methylates Lys189 of the TAF10, a member of the TFIID transcription factor complex, and Lys372 of the p53 tumor suppressor protein (6,7). Methylation of TAF10 stimulates transcription in a promoter-specific manner by increasing the affinity of TAF10 for RNA polymerase II, which may potentiate pre-initiation complex formation (6). Methylation of p53 at Lys372 increases protein stability and leads to upregulation of target genes such as p21. Thus the loss of SET7/SET9 may represent another mechanism for the inactivation of p53 in human cancers (7).

Ribosomal protein L29 (RPL29) is a ubiquitously expressed protein subunit of the cytoplasmic eukaryotic large 60S ribosomal subunit that functions in the translation of RNA to protein. RPL29 is a non-histone substrate of the SET7/SET9 protein methyltransferase and is exclusively mono- and di-methylated on lysine 5 by SET7/SET9. In addition, RPL29 lysine 5 is demethylated by the LSD1 protein demethylase (8). This antibody provides a specific readout for SET7/SET9 methyltransferase and LSD1 demethylase activities.

使用例
 
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Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Di-Methyl-RPL29 (Lys5) (D8T9P) Rabbit mAb

Metabolic Reprogramming in Disease

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