#19984 AsCpf1 (Strain BV3L6) (E1U7C) Rabbit mAb
|トランスフェクトレベルのAsCpf1 (Strain BV3L6) タンパク質を検出します。Cas9 (S. pyogenes)、Cas9 (S. aureus)、FnCpf1 (Strain U112) タンパク質とは交差しません。|
|Acidaminococcus sp. Cpf1 (Strain BV3L6) タンパク質のLeu822 周辺領域 (合成ペプチド)|
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Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing AsCpf1 (Strain BV3L6), FnCpf1 (Strain U112), Cas9 (S. pyogenes), or Cas9 (S. aureus) (+), using AsCpf1 (Strain BV3L6) (E1U7C) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).
Confocal immunofluorescent analysis of 293T cells transfected with a construct expressing myc-tagged AsCpf1 (Strain BV3L6) using AsCpf1 (Strain BV3L6) (E1U7C) Rabbit mAb (green) and Myc-Tag (9B11) Mouse mAb #2276 (red).
Overlapping signals are shown in yellow. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of 293 cells, untransfected (blue) or transfected with myc-tagged AsCpf1 (Strain BV3L6) (green), using AsCpf1 (Strain BV3L6) (E1U7C) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1 (CRISPR from Prevotella and Francisella) are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1 endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1 utilizes T-Rich protospacer adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1 generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1 bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2, 4).
AsCpf1 (Strain BV3L6) is a Cpf1 enzyme derived from Acidaminococcus sp. BV3L6 (5, 6).
The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation, for research use only for immunocytometry, immunohistochemistry, high content screening (HCS) analysis, or flow cytometry applications.
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XP is a registered trademark of Cell Signaling Technology, Inc.
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The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.