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#20459 FoxM1 (D3F2B) Rabbit mAb

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2019年3月20日11時40分 現在
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#20459S100 μL58,000
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FoxM1 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性110Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト -
20459 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫組織染色 (パラフィン) (1:600)クロマチン免疫沈降 (1:100)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのFoxM1 タンパク質を検出します。
使用抗原
ヒトのFoxM1 タンパク質のVal699 周辺領域 (合成ペプチド)

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社内データ

※下記の社内データは、すべて20459 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29, DLD-1, and MCF 10A cells using FoxM1 (D3F2B) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Aphidicolin (2 μM, 24 hr; +), using FoxM1 (D3F2B) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of FoxM1 protein from HT-29 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is FoxM1 (D3F2B) Rabbit mAb. Western blot analysis was performed using FoxM1 (D3F2B) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using FoxM1 (D3F2B) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using FoxM1 (D3F2B) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded U-2 OS cell pellet (left, high-expressing) or Jurkat cell pellet (right, low-expressing) using FoxM1 (D3F2B) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using FoxM1 (D3F2B) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from MCF7 cells, grown in phenol red-free medium and 5% charcoal-stripped FBS for 4 d, followed by treatment with β-estradio (10 nM, 45 min) and either

FoxM1 (D3F2B) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CCNB1 Promoter Primers #70694, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

Forkhead box M1 (FoxM1) is a forkhead box family transcription factor that regulates a number of genes throughout the cell cycle to help control DNA replication, mitosis, and cell proliferation. FoxM1 expression increases during G1 and S and reaches maximum levels in G2/M (1-3). Nuclear translocation occurs just before entry into G2/M and is associated with FoxM1 phosphorylation (4). Phosphorylation of FoxM1 by MAPK (Ser331, Ser704), Cyclin/Cdk (Ser4, Ser35, Thr600, Thr611, Thr620, Thr627, Ser638), Plk1 (Ser715, Ser724), and Chk2 (Ser376) stabilizes and activates FoxM1 (4-8). Forkhead box M1 is expressed in all embryonic tissues but is restricted to proliferating tissues in adults (9). Research studies show that FoxM1 expression is negatively regulated by p53 (10,11). Upregulation of FoxM1 is associated with many human cancers, including prostate, breast, lung, ovary, colon, pancreas, stomach, bladder, liver, and kidney, and may be associated with p53 mutations in some tumors (11,12). As a result, FoxM1 inhibitors have become a topic of interest for potential cancer therapy (13).

使用例
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
14166   Hematoxylin
14170   Phospho-FoxM1 (Ser35) Antibody
14177   SignalStain® Mounting Medium
14655   Phospho-FoxM1 (Thr600) (D9M6G) Rabbit mAb
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
4486   SimpleChIP® Human α Satellite Repeat Primers
5436   FoxM1 (D12D5) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7017   6-Tube Magnetic Separation Rack
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

FoxM1 (D3F2B) Rabbit mAb

Metabolic Reprogramming in Disease

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