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#23214 SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific)

 

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CSTコード 包装
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2019年1月18日15時10分 現在
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SQSTM1 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性62Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

マウス、ラット -
23214 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:200)免疫組織染色 (パラフィン) (1:250)免疫蛍光細胞染色 (IF-IC) (1:800)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのSQSTM1/p62 タンパク質を検出します。
使用抗原
マウスのSQSTM1/p62 タンパク質のGly300 周辺領域 (合成ペプチド)

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社内データ

※下記の社内データは、すべて23214 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from MEFs from wild-type or SQSTM1/p62 knockout mice using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). MEF SQSTM1/p62 KO cells were kindly provided by Dr. Junying Yuan, Harvard Medical School, Boston MA.

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 cells, untreated (-) or treated with Chloroquine #14774 (50 μM, overnight) using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).


Western Blotting

Western Blotting

Western blot analysis of extracts from MEFs, untreated (-) or treated with Earles Basic Salt Solution (EBSS; 4 hr; +) using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of SQSTM1 from L-929 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific). Western blot was performed using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific). Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MEF wild-type cell pellet (left, positive) or MEF SQSTM1/p62 KO cell pellet (right, negative) using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific). MEF SQSTM1/p62 KO cells were kindly provided by Dr. Junying Yuan, Harvard Medical School, Boston MA.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse forestomach using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse kidney using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse spleen using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded rat spleen using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse small intestine using SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific).

IF-IC

IF-IC

Confocal immunofluorescent analysis of wild-type MEFs, either untreated (left) or treated with Chloroquine #14774 (50 μM, 18 hours; center), and SQSTM1/p62 knock-out MEFs treated with chloroquine (right), using SQSTM1 (D6M5X) Rabbit mAb (green). Actin filaments were labeled with β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). MEF SQSTM1/p62 KO cells were kindly provided by Dr. Junying Yuan, Harvard Medical School, Boston MA.


バックグラウンド

Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5) and independently found to interact with PKCζ (6,7). SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity.

使用例
 
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Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

SQSTM1/p62 (D6M5X) Rabbit mAb (Rodent Specific)

Immune Cell Signaling Pathways

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