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#24591 PhosphoPlus® DARPP-32 (Thr34) Antibody Duet

 
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希望納入価格 (円)
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2019年3月22日15時35分 現在
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キット内容 容量 用途 種交差性 ホモロジー† Mol. Wt. アイソタイプ
Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb #12438 100 µl WB H Mouse, Rat 32 kDa Rabbit IgG
DARPP-32 (19A3) Rabbit mAb #2306 100 µl WB, IP, IHC-P, IF-F M, R Human 32 kDa Rabbit

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen)

Reactivity Key: H=Human, M=Mouse, R=Rat

Kit 情報
Total 及び活性化特異的抗体が含まれ、ターゲットタンパク質の活性化状態の測定に最適です。CST抗体の中でお薦めする、最高品質の抗体をセットにしました。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from rat and mouse brain using DARPP-32 (19A3) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from MKN-45 cells, untreated (-) or treated with Forskolin #3828 (30 μM, 20 min; +), or nonphospho-DARPP-32 and phospho-DARPP-32 (Thr34) recombinant proteins using Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb (upper) or DARPP-32 (19A3) Rabbit mAb #2306 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from rat, mouse brain, wild-type mouse striatum and DARPP-32 knock-out mouse striatum, using DARPP-32 (19A3) Rabbit mAb (wild-type mouse striatum and DARPP-32 knock-out mouse striatum was kindly provided by Dr. Paul Greengard and Helen Bateup, Rockefeller University, New York, NY).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain using DARPP-32 (19A3) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded rat retina using DARPP-32 (19A3) Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of normal rat cerebellum, retina, and ciliary epithelium using DARPP-32 (19A3) Rabbit mAb (green) and Neurofilament-H (RMdO 20) Mouse mAb #2836 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


バックグラウンド

DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).

使用例
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関連製品
12134   CDK5 (1H3) Mouse mAb
12438   Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
14145   CDK5 (D1F7M) Rabbit mAb
2301   Phospho-DARPP-32 (Thr75) Antibody
2306   DARPP-32 (19A3) Rabbit mAb
2506   CDK5 Antibody
2680   p35/25 (C64B10) Rabbit mAb
3828   Forskolin
5661   Phospho-PKA C (Thr197) (D45D3) Rabbit mAb
5675   PKA RI-α (D54D9) Rabbit mAb
5842   PKA C-α (D38C6) Rabbit mAb
6883   SignalFire™ ECL Reagent
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9624   Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

DRAQ5 is a registered trademark of Biostatus Limited.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

PhosphoPlus® DARPP-32 (Thr34) Antibody Duet

Immune Cell Signaling Pathways

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