CST logo

#27901 XBP-1s (E8C2Z) Mouse mAb

Shopping Days対象製品 対象製品の中から、まとめて購入で一部製品が無料に!
*ウェブで専用注文書を作成し、代理店へご注文ください。

をクリックすると選べます。

CSTコード 包装
希望納入価格 (円)
Shopping Daysで購入
国内在庫 i
2019年3月22日15時35分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#27901S100 μL56,000
ログインすると国内在庫状況がご確認いただけます。

XBP1 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性60Mouse IgG2a-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト -
27901 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫蛍光細胞染色 (IF-IC) (1:100)フローサイトメトリー (1:1600)クロマチン免疫沈降 (1:200)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
XBP-1s (E8C2Z) Mouse mAb recognizes endogenous levels of total XBP-1s protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala285 of human XBP-1s protein.

ホモロジー (相同性) 検索をご希望の場合 >>>

ホモロジー検索をご要望の際は、ご希望のサンプル種のアミノ酸配列とともにお問合せください。

社内データ

※下記の社内データは、すべて27901 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untreated (-) or treated with tunicamycin #12819 (2 μg/ml, 8 hr; +), using XBP-1s (E8C2Z) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-AS cells, untreated (-) or treated with thapsigargin #12758 (1 μM, 8 hr; +), using XBP-1s (E8C2Z) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IF-IC

IF-IC

Confocal immunofluorescent analysis of SK-N-AS cells, untreated (left) or treated with thapsigargin #12758 (1 μM, 8 hr; right), using XBP-1s (E8C2Z) Mouse mAb (green) and β-Actin (13E5) Rabbit mAb #4970 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of 293T cells, untreated (blue) or treated with tunicamycin #12819 (2 μg/ml, 7 hr; green), using XBP-1s (E8C2Z) Mouse mAb (solid lines) or concentration-matched Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells treated with tunicamycin #12819 (2 μg/ml, 8 hr) and either XBP-1s (E8C2Z) Mouse mAb #27901 or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human DNAJB9 Exon 1 Primers #79879, human HSPA5 promoter primers, human HNRNPA3 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

Following protein synthesis, secretory, intra-organellar, and transmembrane proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. The accumulation of unfolded proteins within the ER triggers an adaptive mechanism known as the unfolded protein response (UPR) that counteracts compromised protein folding (1). The transmembrane serine/threonine kinase IRE1, originally identified in Saccharomyces cerevisiae, is a proximal sensor for the UPR that transmits the unfolded protein signal across the ER membrane (2-4). The human homolog IRE1α was later identified and is ubiquitously expressed in human tissues (5). Upon activation of the unfolded protein response, IRE1α splices X-box binding protein 1 (XBP-1) mRNA through an unconventional mechanism using its endoribonuclease activity (6). This reaction converts XBP-1 from an unspliced XBP-1u isoform to the spliced XBP-1s isoform, which is a potent transcriptional activator that induces expression of many UPR responsive genes (6).

使用例
 
関連製品
11815   ATF-4 (D4B8) Rabbit mAb
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
12782   XBP-1s (D2C1F) Rabbit mAb
2157   MBTPS2 Antibody
2446   PDI Antibody
2679   Calnexin (C5C9) Rabbit mAb
2798   ERp72 Antibody
2881   ERp57 (G117) Antibody
2886   ERp44 Antibody
2887   ERp57 (A484) Antibody
2895   CHOP (L63F7) Mouse mAb
3177   BiP (C50B12) Rabbit mAb
3183   BiP Antibody
3192   PERK (C33E10) Rabbit mAb
3264   Ero1-Lα Antibody
3294   IRE1α (14C10) Rabbit mAb
3597   Phospho-eIF2α (Ser51) (119A11) Rabbit mAb
4486   SimpleChIP® Human α Satellite Repeat Primers
47134   XBP-1s (E7M5C) Mouse mAb
5554   CHOP (D46F1) Rabbit mAb
5683   PERK (D11A8) Rabbit mAb
65880   ATF-6 (D4Z8V) Rabbit mAb
6883   SignalFire™ ECL Reagent
7017   6-Tube Magnetic Separation Rack
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
79879   SimpleChIP® Human DNAJB9 Exon 1 Primers
83418   XBP-1s Antibody
88989   SimpleChIP® Universal qPCR Master Mix
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
9721   Phospho-eIF2α (Ser51) Antibody
9956   ER Stress Antibody Sampler Kit
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
ProLong is a registered trademark of Life Technologies Corporation.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

XBP-1s (E8C2Z) Mouse mAb

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications