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#29087 PhosphoPlus® α-Synuclein (Ser129) Antibody Duet

 
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年3月25日15時35分 現在
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Synuclein 製品一覧

29087 の推奨プロトコール i

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注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 Mol. Wt. アイソタイプ
Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb #23706 100 µl WB, IP, IF-F H, M, R 18 kDa Rabbit IgG
α-Synuclein (D37A6) XP® Rabbit mAb #4179 100 µl WB, IP, IHC-P, IF-F M, R 18 kDa Rabbit IgG

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen), IHC-P=Immunohistochemistry (Paraffin)

Reactivity Key: H=Human, M=Mouse, R=Rat

Kit 情報
Total 及び活性化特異的抗体が含まれ、ターゲットタンパク質の活性化状態の測定に最適です。CST抗体の中でお薦めする、最高品質の抗体をセットにしました。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using α-Synuclein (D37A6) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, untreated (-) or λ phosphatase-treated (+), using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper) or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain using α-Synuclein (D37A6) XP® Rabbit mAb.


IF-F

IF-F

Confocal immunofluorescent analysis of mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper), or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower) (green), in the presence of Phospho-α-Synuclein (Ser129) peptide (middle) or Non-phospho-α-Synuclein (Ser129) peptide (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of normal rat cerebellum, hippocampus and striatum using α-Synuclein (D37A6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of untreated (left) or λ phosphatase-treated (right) mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper), or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


IF-F

IF-F

Confocal immunofluorescent analysis of mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (green). High-magnification showing punctate and sparse nuclear labeling (arrows). Specificity of nuclear staining is unclear (see Specificity/Sensitivity statement and supporting IF-F validation data). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-synuclein gene are linked to Parkinson's disease (1).

Various research studies have shown that phosphorylation of α-Synuclein at Ser129 is a highly toxic event that causes degeneration of dopaminergic neurons, which are associated with Parkinson's disease. This is proposed to occur through increased misfolding, aggregation, and accumulation of α-Synuclein phosphorylated at this site (2). GSK-3β is one of several kinases that has been reported to phosphorylate α-Synuclein at Ser129 (3).

使用例
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DRAQ5 is a registered trademark of Biostatus Limited.
Tween is a registered trademark of ICI Americas, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

PhosphoPlus® α-Synuclein (Ser129) Antibody Duet

Immune Cell Signaling Pathways

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