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#2915 Wnt Signaling Antibody Sampler Kit

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希望納入価格 (円)
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2019年1月18日15時10分 現在
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#2915T1 Kit115,000
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2915 の推奨プロトコール i

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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Wnt3a (C64F2) Rabbit mAb #2721 20 µl WB, IP M H, R 42 Rabbit IgG
Wnt5a/b (C27E8) Rabbit mAb #2530 20 µl WB H 45 Rabbit IgG
Phospho-LRP6 (Ser1490) Antibody #2568 20 µl WB, IP H M 180, 210 Rabbit
Dvl2 (30D2) Rabbit mAb #3224 20 µl WB, IP H, M, R, Mk 90 to 95 Rabbit
LRP6 (C47E12) Rabbit mAb #3395 20 µl WB, IP H, M R 180, 210 Rabbit IgG
Dvl3 Antibody #3218 20 µl WB, IP H, M, R, Hm, Mk, Mi, B 88 to 93 Rabbit
Naked1 (C30F10) Rabbit mAb #2201 20 µl WB, IP H 59, 61 Rabbit IgG
Naked2 (C67C4) Rabbit mAb #2073 20 µl WB, IP H 59, 61 Rabbit
Axin1 (C76H11) Rabbit mAb #2087 20 µl WB, IP, IHC-P H, M 110 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: M=Mouse, H=Human, R=Rat, Mk=Monkey, Hm=Hamster, Mi=Mink, B=Bovine

貯法
-20℃
※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, C6, A549, BAEC and COS cells using Dvl3 Antibody.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from HeLa cells untreated, treated 5 hours with conditioned medium (CM) from L cells, or treated 5 hours with conditioned medium from L cells overexpressing Wnt3a, using Phospho-LRP6 (Ser1490) Antibody #2568.


Western Blotting

Western Blotting

Western blot analysis of total cell lysates from various cell lines using Dvl2 (30D2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from HepG2 and SW620 cells using Naked1 (C30F10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from HeLa, LN18, PANC1 cells and HeLa cells conditioned medium (CM) using Wnt5a/b (C27E8) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of total cell lysates and conditioned medium from L cells overexpressing mouse Wnt3a, compared with parental L cells, using Wnt3a (C64F2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Axin1 (C76H11) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Naked2 (C67C4) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from Hep G2 and HeLa cells using LRP6 (C47E12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from Rat2 and NIH/3T3 cells, untreated or treated with conditioned medium containing Wnt3a for 60 minutes and also treated with or without calf intestinal phosphatase (CIP), using Dvl2 (30D2) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Axin1 (C76H11) Rabbit mAb.


バックグラウンド

The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (2). Wnt members bind to the Frizzled family of proteins and activate several signaling pathways (3). The canonical Wnt/β-catenin pathway requires a coreceptor from proteins belonging to the low-density lipoprotein receptor (LDLR) family, such as LRP5 and LRP6 (4). Upon stimulation with Wnt, LRP6 is phosphorylated by kinases such as GSK-3 and CK1 and subsequently recruits Axin to the membrane (5-7). In the absence of Wnt stimulation, the multidomain scaffold proteins Axin1 and Axin2 negatively regulate Wnt signaling. Axin1 complexes with APC, GSK-3β, Dvl and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (8-9). The Disheveled (Dsh) proteins (Dvl1, Dvl2 and Dvl3) inhibit glycogen synthase kinase-3β, thereby promoting β-catenin stabilization (4,10). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dsh proteins and directing Dsh activity towards the planar cell polarity pathway (11-12).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2074   Axin1 (C95H11) Rabbit mAb
2151   Axin2 (76G6) Rabbit mAb
2203   TCF1/TCF7 (C63D9) Rabbit mAb
2230   LEF1 (C12A5) Rabbit mAb
2262   Naked1 Antibody
2391   Wnt3a Antibody
2392   Wnt5a Antibody
2504   APC Antibody
2569   TCF4/TCF7L2 (C48H11) Rabbit mAb
2596   Naked2 (C36D3) Rabbit mAb
2883   TCF3/TCF7L1 (D15G11) Rabbit mAb
3216   Dvl2 Antibody
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9561   Phospho-β-Catenin (Ser33/37/Thr41) Antibody
9565   Phospho-β-Catenin (Thr41/Ser45) Antibody
9582   β-Catenin (6B3) Rabbit mAb

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

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