CST logo

#3015 Insulin Receptor Substrate Antibody Sampler Kit

 
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年1月18日15時10分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#3015T1 Kit84,000
ログインすると国内在庫状況がご確認いただけます。
3015 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 
キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-IRS-1 (Ser307) Antibody #2381 20 µl WB H, M, R 180 Rabbit
Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb #3203 20 µl WB H, M, R 180 Rabbit IgG
IRS-2 Antibody #4502 20 µl WB H, M, R 185 Rabbit
Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb #5610 20 µl WB, IP H, M R 180 Rabbit IgG
IRS-1 (D23G12) Rabbit mAb #3407 20 µl WB, IP H, M, R, Mk 180 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from CHO IR/4PS cells (transfected with insulin receptor and IRS-2) or CHO IR/IRS-1 cells (transfected with insulin receptor and IRS-1), showing no change in total protein levels with insulin stimulation (100 nM for 5 min), using IRS-2 Antibody (top) or IRS-1 Antibody #2382 (bottom).

Western Blotting

Western Blotting

Western blot analysis of MCF-7 cell extracts, unstimulated and insulin-stimulated (100 nM for 5 min), using IRS-1 Antibody #2382 (left) and Phospho-IRS-1 (Ser307) Antibody (right).


Western Blotting

Western Blotting

Western blot analysis of cell extracts from CHO IR/IRS-1 cells, untreated or treated with insulin, using Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb (upper and middle) or IRS-1 Antibody #2382 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing.

Western Blotting

Western Blotting

Western blot analysis of MCF7, C2C12 and RD cell lines using IRS-1 (D23G12) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved C2C12 cells, untreated or insulin-treated (150 nM for 5 min.), using Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb (upper), or IRS-1 Antibody #3407 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from CHO IR/IRS-1 cells (transfected with insulin receptor and IRS-1), untreated or insulin-treated (100 nM for 5 min), showing an increase in phospho-IRS-1 (Ser307) with insulin stimulation, using Phospho-IRS-1 (Ser307) Antibody.

バックグラウンド

Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
2384   Phospho-IRS-1 (Ser302) Antibody
2385   Phospho-IRS-1 (Ser1101) Antibody
2386   Phospho-IRS-1 (Ser612) Antibody
2389   Phospho-IRS-1 (Ser789) Antibody
2390   IRS-1 (59G8) Rabbit mAb
2491   Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb
2580   Phospho-IRS-1 (Ser332/336) Antibody
3014   Insulin (C27C9) Rabbit mAb
3066   Phospho-IRS-1 (Tyr1222) Antibody
3070   Phospho-IRS-1 (Tyr895) Antibody
3089   IRS-2 (L1326) Antibody
3193   Phospho-IRS-1 (Ser612) (L7B8) Mouse mAb
3194   IRS-1 (L3D12) Mouse mAb
3203   Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb
7133   PathScan® Phospho-IRS-1 (panTyr) Sandwich ELISA Kit
7287   PathScan® Phospho-IRS-1 (Ser307) Sandwich ELISA Kit
7328   PathScan® Total IRS-1 Sandwich ELISA Kit

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

本製品は試験研究用です。

Insulin Receptor Substrate Antibody Sampler Kit

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications