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#30559 CBX4 (E6L7X) Rabbit mAb

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2019年3月26日15時45分 現在
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CBX4 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性78Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、サル -
30559 の推奨プロトコール i

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推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:100)免疫蛍光細胞染色 (IF-IC) (1:200)クロマチン免疫沈降 (1:50)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
CBX4 (E6L7X) Rabbit mAb recognizes endogenous levels of total CBX4 protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro166 of human CBX4 protein.

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社内データ

※下記の社内データは、すべて30559 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CBX4 (E6L7X) Rabbit mAb. As expected, HDLM2 and NTERA-2 cells do not highly express CBX4.

IP

IP

Immunoprecipitation of CBX4 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CBX4 (E6L7X) Rabbit mAb. Western blot analysis was performed using CBX4 (E6L7X) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of Caki-1 cells using CBX4 (E6L7X) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells and either CBX4 (E6L7X) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human HoxA1 Intron 1 Primers #7707, SimpleChIP® Human HoxA2 Promoter Primers #5517 and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyl-transferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (6). CBX4 is a component of the PRC1 complex, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit (7,8). CBX4 itself is a SUMO E3 ligase, and its function influences EMT, DNA damage response, tumor angiogenesis, and self-renewal (9-13).

使用例
 
関連製品
4486   SimpleChIP® Human α Satellite Repeat Primers
5517   SimpleChIP® Human HoxA2 Promoter Primers
7003   20X LumiGLO® Reagent and 20X Peroxide
7017   6-Tube Magnetic Separation Rack
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7707   SimpleChIP® Human HoxA1 Intron 1 Primers
7727   Biotinylated Protein Ladder Detection Pack
88989   SimpleChIP® Universal qPCR Master Mix
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

CBX4 (E6L7X) Rabbit mAb

Metabolic Reprogramming in Disease

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