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#30874 TPBG/5T4 (E4T8Q) Rabbit mAb

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2019年3月20日11時40分 現在
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#30874S100 μL56,000
感度分子量 (kDa)抗体の由来貯法
内在性75Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


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30874 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)フローサイトメトリー (1:400)


内在性レベルのTPBG/5T4 タンパク質を検出します。
ヒトのTPBG/5T4 タンパク質のHis140 周辺領域 (合成ペプチド)

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※下記の社内データは、すべて30874 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TPBG/5T4 protein (hTPBG/5T4-Myc/DDK; +), using TPBG/5T4 (E4T8Q) Rabbit mAb (upper), DYKDDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with PNGase F (+), using TPBG/5T4 (E4T8Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TPBG/5T4 (E4T8Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, TPBG/5T4 protein is expressed at very low levels in 293T and is not expressed in NCI-H460 cells.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of 293T cells (blue) and MCF7 cells (green) using TPBG/5T4 (E4T8Q) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


Trophoblast glycoprotein (TPBG/5T4) is a type-I transmembrane glycoprotein that is normally expressed by trophoblast cells of the placenta with a very limited expression pattern in normal adult tissues (1,2). The extracellular domain of TPBG is extensively glycosylated and contains multiple leucine-rich repeats while its cytoplasmic domain consists of PDZ domain-binding motif, which is important for linking TPBG to intracellular signaling networks involved in the regulation of cell motility and adhesion (3,4). Research studies have shown that cell surface expression of TPBG plays a critical role in modulating signaling cascades that drive cell adhesion, morphology, and motility processes that are fundamental for normal progression of embryogensis (5-7).

Research studies have demonstrated that TPBG is aberrantly over expressed in numerous types of solid tumors (8) and functions to promote enhanced tumor cell motility and metastasis (9,10). In some tumors, such as NSCLC and HNSCC, TPBG has been identified as novel marker of tumor-initiating cells (11,12). The observed differential expression of TPBG by normal tissue versus tumor tissue has been exploited by multiple immunotherapeutic agents that are currently being evaluated for targeting of multiple types of solid tumors (13).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
3900   Rabbit (DA1E) mAb IgG XP® Isotype Control
6883   SignalFire™ ECL Reagent
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8457   β-Actin (D6A8) Rabbit mAb
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


TPBG/5T4 (E4T8Q) Rabbit mAb

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