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#32098 β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb

 

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2019年2月22日15時50分 現在
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#32098S100 μL66,000
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Amyloid beta 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性6Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス -
32098 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫蛍光染色 (IF-F) (1:50)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb recognizes endogenous levels of total human Aβ-43 protein. This product detects transgenically expressed human APP in mouse models. This antibody weakly cross-reacts with human Aβ-42 protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy of human β-Amyloid (1-43) protein.

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社内データ

※下記の社内データは、すべて32098 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis with the indicated amounts of human β-amyloid (1-43) protein using β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of human Aβ-37, Aβ-38, Aβ-39, Aβ-40, Aβ-42 and Aβ-43 peptides (10 ng) using β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb. Note the slight cross-reactivity with Aβ-42.

IF-F

IF-F

Confocal immunofluorescent analysis of brain from the 5XFAD mouse model of Alzheimer's disease using β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb (green). After blocking free secondary antibody binding sites with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, the tissue was then labeled using GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red pseudocolor) and β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #42284 (yellow pseudocolor). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).


バックグラウンド

Amyloid β (Aβ) precursor protein (APP) is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms (1). The amino acid sequence of APP contains the amyloid domain, which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released Aβ fragments form the main components of amyloid plaques in Alzheimer's disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion of this protein (2-5). Phosphorylation at Thr668 (a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell-cycle dependent and peaks during G2/M phase (4). APP phosphorylated at Thr668 exists in adult rat brain and correlates with cultured neuronal differentiation (5,6).

Aβ43 has been suggested as a biomarker in early onset of Alzheimer's disease, where patients have lower levels of Aβ43 in cerebrospinal fluid (7,8,9). Several studies have shown that Aβ toxicity of Aβ43 is as high as Aβ42 or Aβ40 in different models of Alzheimer's disease, including mouse models and human disease. (10).

使用例
 
関連製品
12077   β-Amyloid (1-39 Specific) (D5Y9L) Rabbit mAb
12467   β-Amyloid (1-37 Specific) (D2A6H) Rabbit mAb
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
12843   β-Amyloid (1-42 Specific) (D3E10) Rabbit mAb
2450   APP/β-Amyloid (NAB228) Mouse mAb
2452   APP Antibody
3656   GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate)
3823   Phospho-APP (Thr668) Antibody
3900   Rabbit (DA1E) mAb IgG XP® Isotype Control
42284   β-Amyloid (D54D2) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
5606   BACE (D10E5) Rabbit mAb
5887   γ Secretase Antibody Sampler Kit
6883   SignalFire™ ECL Reagent
6986   Phospho-APP (Thr668) (D90B8) Rabbit mAb
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8243   β-Amyloid (D54D2) XP® Rabbit mAb
8961   ProLong® Gold Antifade Reagent with DAPI
9071   ProLong® Gold Antifade Reagent
9784   Alzheimer's Disease Antibody Sampler Kit
9888   β-Amyloid (D12B2) Rabbit mAb
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
ProLong is a registered trademark of Life Technologies Corporation.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

β-Amyloid (1-43 Preferred) (E8C2D) Rabbit mAb

Immune Cell Signaling Pathways

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