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#32961 Inflammasome Antibody Sampler Kit

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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#32961T1 Kit115,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
NLRP3 (D4D8T) Rabbit mAb #15101 20 µl WB, IP H, M Hm 110 Rabbit IgG
AIM2 (D5X7K) Rabbit mAb #12948 20 µl WB, IP H Mk 40 Rabbit IgG
NLRC4 (D5Y8E) Rabbit mAb #12421 20 µl WB, IP H 110 Rabbit IgG
Caspase-1 (D7F10) Rabbit mAb #3866 20 µl WB, IP H Mk 48, 20 Rabbit IgG
NALP1 Antibody #4990 20 µl WB H, M, R Mk 165, 70 Rabbit
ASC/TMS1 (E1E3I) Rabbit mAb #13833 20 µl WB, IP H 22, 19, 15 Rabbit IgG
Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199 20 µl WB, IP H Mk 20, 22 Rabbit IgG
Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb #83186 20 µl WB, IP, IF-IC H 17 Rabbit IgG
IL-1β (D3U3E) Rabbit mAb #12703 20 µl WB, IF-IC, F H 17, 31 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Reactivity Key: H=Human, M=Mouse, R=Rat

貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from K-562 and SH-SY5Y cells and from rat testis using NALP1 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 and NK-92 cells using Caspase-1 (D7F10) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, untransfected () or transfected with construct overexpressing human caspase-1 (+), using Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb (upper) or Caspase-1 (D7F10) Rabbit mAb #3866 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 and MUTZ-3 cells using NLRC4 (D5Y8E) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/ml, 3 hr; +), using IL-1β (D3U3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from Daudi, KARPAS-299, and L-540 cell lines using AIM2 (D5X7K) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using ASC/TMS1 (E1E3I) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, Jurkat cells do not express ASC/TMS1.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse bone marrow-derived dendritic cells (BMDC) and various cell lines using NLRP3 (D4D8T) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


Western Blotting

Western Blotting

Western blot analysis of recombinant Human Interleukin-1β (hIL-1β) #8900 using Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with human caspase-1, using Caspase-1 (D7F10) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from the media of THP-1 cells, differentiated with TPA #9905 (80 nM, overnight) followed by treatment with LPS (1 mu-g/ml, 8 hours), using Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human NLRC4 (hNLRC4; +), using NLRC4 (D5Y8E) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of recombinant Human Interleukin-1β (hIL-1β) #8900 using IL-1β (D3U3E) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HL-60 cells, serum-starved and either untreated (-) or treated overnight with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml; +), using AIM2 (D5X7K) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).


IP

IP

Immunoprecipitation of ASC/TMS1 from THP-1 cell extracts using Rabbit (D1AE) mAb XP® Isotype Control #3900 (lane 2) or ASC/TMS1 (E1E3I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using ASC/TMS1 (E1E3I) Rabbit mAb.

IP

IP

Immunoprecipitation of NLRP3 from J774A.1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or NLRP3 (D4D8T) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NLRP3 (D4D8T) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from cells or media collected from THP-1 cells, differentiated with TPA #4147 (80 nM, overnight) and subsequently treated with (+) or without (-) Lipopolysaccharides (LPS) #14011 (1 μg/ml, 6 hr), using Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb.


IP

IP

Immunoprecipitation of NLRC4 from MUTZ-3 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or NLRC4 (D5Y8E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using NLRC4 (D5Y8E) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of THP-1 cells, untreated (blue) or LPS-treated (100 ng/ml, 3 hr; green), using IL-1β (D3U3E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-DDK tagged human AIM2 (hAIM2-Myc/DDK; +), using AIM2 (D5X7K) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).


IP

IP

Immunoprecipitation of Cleaved-IL-1β (Asp116) from extracts of THP-1 cells differentiated with TPA #4147 (80 nM, overnight) followed by treatment with Lipopolysaccharides (LPS) #14011 (1 μg/ml, 6 hr). Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is precipitated with Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb. Western blot was performed using Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of THP-1 cells, untreated (left) or LPS-treated (500 ng/ml, 2 hr; right), using IL-1β (D3U3E) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

IP

IP

Immunoprecipitation of AIM2 from HL-60 cell extracts treated with Human Interferon-γ (hIFN-γ) #8901 (100 ng/ml, overnight) using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or AIM2 (D5X7K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using AIM2 (D5X7K) Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of THP-1 cells, differentiated with TPA #4174 (80 nM, 24 hr) and subsequently treated with (right) or without (left) Lipopolysaccharides (LPS) #14011 (1 μg/ml, 6 hr), using Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

バックグラウンド

The innate immune system works as the first line of defense in protection from pathogenic microbes and host-derived signals of cellular distress. One way in which these “danger” signals trigger inflammation is through activation of inflammasomes, which are multiprotein complexes that assemble in the cytosol after exposure to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) and result in the activation of caspase-1 and subsequent cleavage of proinflammatory cytokines IL-1β and IL-18 (Reviewd in 1-6). Inflammasome complexes typically consist of a cytosolic pattern recognition receptor (PRR; a nucleotide-binding domain and leucine-rich-repeat [NLR] or AIM2-like receptor [ALR] family member), an adaptor protein (ASC/TMS1), and pro-caspase-1. A number of distinct inflammasome complexes have been identified, each with a unique PRR and activation triggers. The best characterized is the NLRP3 complex, which contains NLRP3, ASC/TMS1, and pro-caspase-1. The NLRP3 inflammasome is activated in a two-step process. First, NF-κB signaling is induced through PAMP- or DAMP-mediated activation of TLR4 or TNFR, resulting in increased expression of NLRP3, pro-IL-1β, and pro-IL-18 (priming step, signal 1). Next, indirect activation of NLRP3 occurs by a multitude of signals (whole pathogens, PAMPs/DAMPs, potassium efflux, lysosomal-damaging environmental factors [uric acid, silica, alum] and endogenous factors [amyloid-β, cholesterol crystals], and mitochondrial damage), leading to complex assembly and activation of caspase-1 (signal 2). The complex inflammasome structure is built via domain interactions among the protein components. Other inflammasomes are activated by more direct means: double-stranded DNA activates the AIM2 complex, anthrax toxin activates NLRP1, and bacterial flagellin activates NLRC4. Activated caspase-1 induces secretion of proinflammatory cytokines IL-1β and -18, but also regulates metabolic enzyme expression, phagosome maturation, vasodilation, and pyroptosis, an inflammatory programmed cell death. Inflammasome signaling contributes to the onset of a number of diseases, including atherosclerosis, type II diabetes, Alzheimer’s disease, and autoimmune disorders.

使用例
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関連製品
12421   NLRC4 (D5Y8E) Rabbit mAb
12703   IL-1β (D3U3E) Rabbit mAb
12948   AIM2 (D5X7K) Rabbit mAb
13833   ASC/TMS1 (E1E3I) Rabbit mAb
15101   NLRP3 (D4D8T) Rabbit mAb
3866   Caspase-1 (D7F10) Rabbit mAb
4199   Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb
4990   NALP1 Antibody
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
83186   Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb

Tween is a registered trademark of ICI Americas, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

本製品は試験研究用です。

Inflammasome Antibody Sampler Kit

Immune Cell Signaling Pathways

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