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#35005 EHMT1 (E6Q8B) Rabbit mAb

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2019年3月22日15時35分 現在
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#35005S100 μL56,000
感度分子量 (kDa)抗体の由来貯法
内在性180Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


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ウェスタンブロッティング (1:1000)免疫沈降 (1:50)


EHMT1 (E6Q8B) Rabbit mAb recognizes endogenous levels of total EHMT1 protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala196 of human EHMT1 protein.

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※下記の社内データは、すべて35005 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF7 and DLD-1 cell lines using EHMT1 (E6Q8B) Rabbit mAb.



Immunoprecipitation of EHMT1 from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is EHMT1 (E6Q8B) Rabbit mAb. Western blot analysis was performed using EHMT1 (E6Q8B) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.


Euchromatic histone-lysine N-methyltransferase 1 (EHMT1), also referred to as G9a-like protein 1 (GLP), is a histone methyltransferase that specifically mono- and dimethylates lysine 9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. EHMT1 is a member of a family of histone lysine methyltransferases that contain a conserved catalytic SET domain originally identified in Drosophila Su(var)3-9, enhancer of zeste and trithorax proteins (1). Although EHMT1 can homodimerize in vitro, it is typically found as a heterodimer in vivo with its paralog G9a, also known as EHMT2; together these proteins function as the major euchromatic histone H3 Lys9 mono- and dimethyltransferase, creating transcriptionally repressive marks that facilitate gene silencing (2,3). The EHMT1/G9a complex also contains Wiz, a zinc finger protein that is required for EHMT1/G9a heterodimerization and complex stability (4). Wiz contains two CtBP co-repressor binding sites, which mediate the association of EHMT1/G9a with the CtBP co-repressor complex (4). In addition, EHMT1 and G9a are components of other large transcriptional co-repressor complexes, such as those involving E2F6 and CDP/cut (5-7). EHMT1/G9a methylates several non-histone substrates, including DNMT1, CEBP/β, HDAC1 (8), and p53, specifically at lysine 373 (9). p53 Lys373 methylation was initially reported to inhibit p53 activity, suggesting that EHMT1/G9a inhibitors could help treat p53-positive cancers (9). However, a more recent study found that human G9a activated p53 and that elevated G9a-p53 expression was associated with increased survival in lung cancer patients, suggesting that further studies are required to better understand the biological significance of this methylation event (10). Lastly, defects in EHMT1 are the cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome), also known as Kleefstra syndrome (KS). Common features of KS patients are moderate to severe intellectual disability, autism, epileptic seizures, microcephaly, and dysmorphic features (11).

2524   p53 (1C12) Mouse mAb
2527   p53 (7F5) Rabbit mAb
3306   G9a/EHMT2 (C6H3) Rabbit mAb
34589   HDAC1 (D5C6U) XP® Rabbit mAb
48818   p53 (DO-7) Mouse mAb
5032   DNMT1 (D63A6) XP® Rabbit mAb
64503   DNMT1 (D63A6) XP® Rabbit mAb (PE Conjugate)
68851   G9a/EHMT2 (D5R4R) XP® Rabbit mAb
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8684   CtBP1 (D2D6) Rabbit mAb
8694   Lysine Methyltransferase Antibody Sampler Kit
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


EHMT1 (E6Q8B) Rabbit mAb

Immune Cell Signaling Pathways