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#3633 ALK (D5F3®) XP® Rabbit mAb

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CSTコード 包装
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国内在庫 i
2019年3月22日15時35分 現在
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#3633S100 μL170,000
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#3633T20 μL59,000

ALK 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性220 (ALK), 80 (NPM-ALK), 117 (EML4-ALK v1), 86 (EML4-ALK v3)Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト -
3633 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:2000)免疫沈降 (1:100)免疫組織染色 (パラフィン) (1:250)フローサイトメトリー (1:400)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのALK タンパク質 (EML4-ALK 変異やNPM-ALK などのALK 融合タンパク質も含む) を検出します。低発現レベルでも検出します。他のファミリータンパク質とは交差しません。
使用抗原
ヒトのALK タンパク質のC末端周辺領域 (組換えタンパク質)

ホモロジー (相同性) 検索をご希望の場合 >>>

ホモロジー検索をご要望の際は、ご希望のサンプル種のアミノ酸配列とともにお問合せください。

社内データ

※下記の社内データは、すべて3633 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from NCI-H2228 and NCI-H3122 cells using ALK (D5F3®) XP® Rabbit mAb. Variants denoting fusions of different EML4 exons (v1 or v3) are indicated.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma with high (left) and low levels (right) of ALK expression using ALK (D5F3®) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded anaplastic large cell lymphoma (ALCL) using ALK (D5F3®) XP® Rabbit mAb.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of DU 145 cells (blue) and KARPAS-299 cells (green) using ALK (D5F3®) XP® Rabbit mAb. Cell Line Source: Dr Abraham Karpas at the University of Cambridge.

バックグラウンド

Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as a nucleophosmin (NPM)-ALK fusion protein produced by a translocation (4). Investigators have found that the NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Research literature suggests that activation of PLCγ by NPM-ALK may be a crucial step for its mitogenic activity and involved in the pathogenesis of anaplastic lymphomas (5).

A distinct ALK oncogenic fusion protein involving ALK and echinoderm microtubule-associated protein like 4 (EML4) has been described in the research literature from a non-small cell lung cancer (NSCLC) cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6-8).

Investigators have identified ALK translocations with other fusion partners, such as TRK-fused gene (TFG) and KIF5B, which have also been associated with NSCLC (6,7). In particular, the EML4-ALK fusion protein has been found in 3-7% of NSCLC samples (6-14).

使用例
 
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※The purchase of this product conveys exclusively to the buyer a non-transferable limited license to the purchased amount of the product and to its use under one or more patents, patent applications and/or trade secrets proprietary to Cell Signaling Technology, Inc. for IN VITRO AND INTERNAL RESEARCH ONLY. No other right or license is granted to the buyer, explicitly or by implication. The sale of this product is expressly conditioned on not using the product or its components (1) to provide a service, information or data to a third party; (2) for therapeutic, diagnostic or prophylactic purposes; (3) to analyze or reverse engineer the product for its chemical/physical properties and composition; (4) for manufacturing; (5) for resale or transfer in any form to a third party; and/or (6) for any other commercial purpose. For information on the availability of licenses for other purposes, contact Cell Signaling Technology, Inc. at licensing@cellsignal.com.

DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
D5F3 is a registered trademark of Cell Signaling Technology. Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

ALK (D5F3®) XP® Rabbit mAb

Metabolic Reprogramming in Disease

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