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#37377 PHF8 (E9R4F) Rabbit mAb

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2019年3月25日15時35分 現在
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#37377S100 μL56,000
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PHF8 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性135, 140Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

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37377 の推奨プロトコール i

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CST推奨プロトコールに欠かせない関連製品

特異性・感度
PHF8 (E9R4F) Rabbit mAb recognizes endogenous levels of total PHF8 protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with recombinant protein containing Phe579 of human PHF8 protein.

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社内データ

※下記の社内データは、すべて37377 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PHF8 (E9R4F) Rabbit mAb.

IP

IP

Immunoprecipitation of PHF8 from MOLT-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PHF8 (E9R4F) Rabbit mAb. Western blot analysis was performed using PHF8 (E9R4F) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of DU 145 cells (blue) and NTERA-2 cells (green) using PHF8 (E9R4F) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L) F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from SH-SY5Y cells and either PHF8 (E9R4F) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human JARID1C Exon 1 Primers #52773, human ZNF41 exon 1 Primers, human promoter ZNF81 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

PHD finger protein 8 (PHF8) is a histone lysine demethylase that functions as a transcriptional activator by specifically demethylating a number of repressive histone methylation marks: mono- and di-methyl-histone H3 Lys9 (H3K9me1 and H3K9me2), di-methyl-histone H3 Lys27 (H3K27me2) and mono-methyl-histone H4 Lys20 (H4K20me1). PHF8 contains an N-terminal zinc finger-like PHD domain that binds tri-methylated histone H3 Lys4 (H3K4Me3) and a C-terminal jumonji domain that is responsible for the demethylase activity (1). Deletion and point mutations (F279S) in the jumonji domain of PHF8 are associated with the onset of X-linked mental retardation (XLMR). In addition, PHF8 is highly expressed in prostate cancer, laryngeal squamous cell carcinoma, and human non-small-cell lung cancer (NSCLC). Its expression is predictive of poor survival (2-4). Overexpression of PHF8 increases cell proliferation and cell motility, while silencing of PHF8 reduces cell proliferation, migration, and invasion (4).

使用例
 
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Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

PHF8 (E9R4F) Rabbit mAb

Immune Cell Signaling Pathways

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