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#37377 PHF8 (E9R4F) Rabbit mAb

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2019年3月25日15時35分 現在
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#37377S100 μL56,000

PHF8 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性135, 140Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


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37377 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)フローサイトメトリー (1:100)クロマチン免疫沈降 (1:50)


PHF8 (E9R4F) Rabbit mAb recognizes endogenous levels of total PHF8 protein.
Monoclonal antibody is produced by immunizing animals with recombinant protein containing Phe579 of human PHF8 protein.

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※下記の社内データは、すべて37377 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PHF8 (E9R4F) Rabbit mAb.



Immunoprecipitation of PHF8 from MOLT-4 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is PHF8 (E9R4F) Rabbit mAb. Western blot analysis was performed using PHF8 (E9R4F) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of DU 145 cells (blue) and NTERA-2 cells (green) using PHF8 (E9R4F) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L) F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from SH-SY5Y cells and either PHF8 (E9R4F) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human JARID1C Exon 1 Primers #52773, human ZNF41 exon 1 Primers, human promoter ZNF81 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


PHD finger protein 8 (PHF8) is a histone lysine demethylase that functions as a transcriptional activator by specifically demethylating a number of repressive histone methylation marks: mono- and di-methyl-histone H3 Lys9 (H3K9me1 and H3K9me2), di-methyl-histone H3 Lys27 (H3K27me2) and mono-methyl-histone H4 Lys20 (H4K20me1). PHF8 contains an N-terminal zinc finger-like PHD domain that binds tri-methylated histone H3 Lys4 (H3K4Me3) and a C-terminal jumonji domain that is responsible for the demethylase activity (1). Deletion and point mutations (F279S) in the jumonji domain of PHF8 are associated with the onset of X-linked mental retardation (XLMR). In addition, PHF8 is highly expressed in prostate cancer, laryngeal squamous cell carcinoma, and human non-small-cell lung cancer (NSCLC). Its expression is predictive of poor survival (2-4). Overexpression of PHF8 increases cell proliferation and cell motility, while silencing of PHF8 reduces cell proliferation, migration, and invasion (4).

13919   Histone H4 (D2X4V) Rabbit mAb
14149   Histone H4 (D2X4V) Rabbit mAb (ChIP Formulated)
14186   Mono-Methyl-Histone H3 (Lys9) (D1P5R) Rabbit mAb
3497   PHF2 (D45A2) Rabbit mAb
3934   PHF20 (D96F6) XP® Rabbit mAb
4486   SimpleChIP® Human α Satellite Repeat Primers
4499   Histone H3 (D1H2) XP® Rabbit mAb
4620   Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated)
4658   Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb
56383   SimpleChIP® Plus Sonication Chromatin IP Kit
5737   Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
7003   20X LumiGLO® Reagent and 20X Peroxide
7017   6-Tube Magnetic Separation Rack
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
77271   PHF19 Antibody
78781   Di-Methyl-Histone H4 (Lys20) (D2D9P) Rabbit mAb
84932   Mono-Methyl-Histone H3 (Lys27) (D3R8N) Rabbit mAb
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
9728   Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb
9751   Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


PHF8 (E9R4F) Rabbit mAb

Immune Cell Signaling Pathways