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#38461 Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit

 
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2019年1月17日11時55分 現在
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
IL-1β (D3U3E) Rabbit mAb #12703 20 µl WB, IF-IC, F H 17, 31 Rabbit IgG
RANTES (R40) Antibody #2987 20 µl WB H Mk 10 Rabbit
CXCL10 (D5L5L) Rabbit mAb #14969 20 µl WB H 10 Rabbit IgG
PAI-1 (D9C4) Rabbit mAb #11907 20 µl WB H, Mk, B 48 Rabbit IgG
IL-6 (D3K2N) Rabbit mAb #12153 20 µl WB, IP H 21-28 Rabbit IgG
TNF-α (D5G9) Rabbit mAb #6945 20 µl WB, IP H 18, 25 Rabbit IgG
MMP3 (D7F5B) Rabbit mAb #14351 20 µl WB H, R 60 Rabbit IgG
MCP-1 Antibody (Carboxy-terminal Antigen) #39091 20 µl WB, IP H 13-15 Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry, IP=Immunoprecipitation
Reactivity Key: H=Human, Mk=Monkey, B=Bovine, R=Rat

特異性・感度
Each antibody in the Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit detects endogenous levels of its target protein.CXCL10 (D5L5L) Rabbit mAb also cross-reacts with a 100kDa protein of unknown origin in some cell lines.
使用抗原
Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Arg294 of human PAI-1 protein, residues surrounding Ser417 of human MMP3 protein, recombinant human IL-1β protein, recombinant human CXCL10 protein, recombinant human IL-6 protein, and recombinant human TNF-α protein.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg40 of human RANTES and near the carboxy terminus of human MCP-1 protein.

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貯法
-20℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from differentiated THP-1 cells, untreated or treated overnight with LPS, using RANTES (R40) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from the media of THP-1 cells differentiated with TPA #4174 (80 nM; overnight), with or without LPS (1 μg/ml; overnight), using TNF-α (D5G9) Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 and HUVEC cells using PAI-1 (D9C4) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of 1 ng recombinant Human Interleukin-6 (hIL-6) #8904 using IL-6 (D3K2N) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, untreated (-) or LPS-treated (100 ng/ml, 3 hr; +), using IL-1β (D3U3E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MMP3 (D7F5B) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of recombinant human CXCL10 protein using CXCL10 (D5L5L) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing human MCP-1 protein (hMCP-1; +), using MCP-1 Antibody (Carboxy-terminal Antigen) (upper) or β-actin (D6A8) Rabbit mab #8457 (lower).


Western Blotting

Western Blotting

Western blot analysis of HeLa cells, mock transfected or transfected with human RANTES construct, using RANTES (R40) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM; overnight), with or without LPS (1 μg/ml; various time points), using TNF-α (D5G9) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells differentiated with TPA #4174 (80 nM, overnight), untreated (-) or LPS-treated (100 ng/ml, 6 hr; +) and treated with Brefeldin A #9972 (300 ng/mL, last 3 hr of stimulation; +), using IL-6 (D3K2N) Rabbit mAb or β-Actin (D6A8) Rabbit mAb #8457 (lower).


Western Blotting

Western Blotting

Western blot analysis of recombinant Human Interleukin-1β (hIL-1β) #8900 using IL-1β (D3U3E) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with Human Interferon-γ (hIFN-γ) #8901 (50 ng/ml, 16 hr; +), using CXCL10 (D5L5L) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from A549 cells, untreated (-) or treated with TNF-α (20 ng/ml, 6 hr; +), using MCP-1 Antibody (Carboxy-terminal Antigen) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).


Western Blotting

Western Blotting

Western blot analysis of recombinant human TNF-α #8902 using TNF-α (D5G9) Rabbit mAb (left) or TNF-α Antibody #3707 (right).

IP

IP

Immunoprecipitation of IL-6 from NCI-H460 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IL-6 (D3K2N) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IL-6 (D3K2N) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of THP-1 cells, untreated (blue) or LPS-treated (100 ng/ml, 3 hr; green), using IL-1β (D3U3E) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.


IP

IP

Immunoprecipitation of MCP-1 protein from conditioned medium of A549 cells treated with TNF-α (20 ng/ml, 6 hr). Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is MCP-1 Antibody (Carboxy-terminal Antigen). Western blot analysis was performed using MCP-1 Antibody (Carboxy-terminal Antigen).

IF-IC

IF-IC

Confocal immunofluorescent analysis of THP-1 cells, untreated (left) or LPS-treated (500 ng/ml, 2 hr; right), using IL-1β (D3U3E) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

バックグラウンド

Senescence is characterized by stable stress-induced proliferative arrest and resistance to mitogenic stimuli, as well as the secretion of proteins such as cytokines, growth factors and proteases. These secreted proteins comprise the senescence-associated secretory phenotype (SASP). Senescent cells are thought to accumulate as an organism ages, and contribute to age-related diseases, including cancer, through promotion of inflammation and disruption of normal cellular function (1,2). The composition of the SASP varies, and SASP components can be either beneficial or deleterious in human disease, depending on the context (3).

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit provides a collection of antibodies to various SASP components, including TNF-alpha, interleukin-6 (IL-6), the multifunctional cytokine IL-1beta, the chemokines CXCL10, RANTES/CCL5 and MCP-1, the matrix metalloprotease MMP3, and the serine-protease inhibitor PAI-1.

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
11907   PAI-1 (D9C4) Rabbit mAb
12153   IL-6 (D3K2N) Rabbit mAb
12703   IL-1β (D3U3E) Rabbit mAb
14351   MMP3 (D7F5B) Rabbit mAb
14969   CXCL10 (D5L5L) Rabbit mAb
2987   RANTES (R40) Antibody
39091   MCP-1 Antibody (Carboxy-terminal Antigen)
6945   TNF-α (D5G9) Rabbit mAb
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

Tween is a registered trademark of ICI Americas, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Senescence Associated Secretory Phenotype (SASP) Antibody Sampler Kit

Immune Cell Signaling Pathways

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