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#3860 PLCγ Antibody Sampler Kit

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希望納入価格 (円)
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2019年1月17日15時10分 現在
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#3860T1 Kit84,000
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キット内容 容量 用途 種交差性 ホモロジー† 検出分子量 アイソタイプ
Phospho-PLCγ1 (Tyr783) Antibody #2821 20 µl WB, F H, M, R 155 Rabbit
Phospho-PLCγ2 (Tyr1217) Antibody #3871 20 µl WB H, M R 150 Rabbit
PLCγ2 Antibody #3872 20 µl WB, IP H, M R 150 Rabbit
Phospho-PLCγ2 (Tyr759) Antibody #3874 20 µl WB H, M 150 Rabbit
PLCγ1 (D9H10) XP® Rabbit mAb #5690 20 µl WB, IP, IHC-P H, M, R, Mk 150 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl WB Goat

†Species predicted to react based on 100% sequence homology.

Applications Key: W=Western Blotting, F=Flow Cytometry, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey

貯法
-20℃
※括弧付きの動物種は配列が100%相同であるため反応すると推定されます。
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos (lane 1), THP1 (lane 2), U-937 (lane 3) cells and mouse spleenocytes (lane 4), using PLCγ2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos cells, untreated or treated with anti-human IgM, using Phospho-PLCgamma2 (Tyr1217) Antibody (upper) or PLCgamma2 Antibody #3872 (lower).


Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-stimulated for the indicated times, using Phospho-PLCγ1 (Tyr783) Antibody (upper) or PLCγ1 Antibody #2822 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or anti-human IgM treated Ramos cells or lamda phosphatase-treated Ramos cell lysates, using Phospho-PLCgamma2 (Tyr759) Antibody (upper) or PLCgamma2 Antibody #3872 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PLCγ1 (D9H10) XP® Rabbit mAb.


Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 (lanes 1, 3, 5) and Ramos (lanes 2, 4, 6) cells, using PLCγ1 Antibody #2822 (lanes 1 and 2), PLCgamma2 Antibody (lanes 5 and 6) or both antibodies (lanes 3 and 4). Results show that PLCγ2 Antibody is specific to the 150 kDa PLCγ2 band detected in Ramos cells, while PLCγ1 Antibody #2822 is specifc to the 160 kDa PLCγ1 band detected in both Ramos and NIH/3T3 cells.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH3T3 cells, untreated (blue) or PDGF treated (green), using Phospho-PLCγ1 (Tyr783) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, either mock-transfected or transfected for 48 hours with SignalSilence™ PLCγ1 siRNA I #6293 or siRNA II #6254, using PLCγ1 (D9H10) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PLCγ1 (D9H10) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcioma using PLCγ1 (D9H10) XP® Rabbit mAb.

バックグラウンド

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).

使用例
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XP is a registered trademark of Cell Signaling Technology, Inc.
LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

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