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#38896 MAGE-A3 (E9S4X) Rabbit mAb

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2019年3月20日11時40分 現在
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MAGE-A3 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性45Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト -
38896 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:50)

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特異性・感度
MAGE-A3 (E9S4X) Rabbit mAb recognizes endogenous levels of total MAGE-A3 protein. This antibody cross-reacts with MAGE-A2 and MAGE-A12 proteins.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAGE-A3 protein.

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社内データ

※下記の社内データは、すべて38896 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MAGE-A3 (E9S4X) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, MAGE-A3 protein is not detected in HCC1143 cells.

Western Blotting

Western Blotting

Western blot anlysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing Myc/DDK-tagged full-length human MAGE-A2 (hMAGE-A2-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A3 (hMAGE-A3-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A4 (hMAGE-A4-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A6 (hMAGE-A6-Myc/DDK), Myc/DDK-tagged full-length human MAGE-A10 (hMAGE-A10-Myc/DDK), and Myc/DDK-tagged full-length human MAGE-A12 (hMAGE-A12-Myc/DDK), using MAGE-A3 (E9S4X) Rabbit mAb (upper), DYKDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of MAGE-A3 from SK-MEL-28 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MAGE-A3 (E9S4X) Rabbit mAb. Western blot analysis was performed using MAGE-A3 (E9S4X) Rabbit mAb. Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used for detection to avoid cross-reactivity with IgG.


バックグラウンド

Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3).

Melanoma antigen-A3 (MAGE-A3) is a cancer testis antigen and belongs to the type I MAGE family of proteins. The expression of MAGE-A3 is normally restricted to the human testis but is aberrantly upregulated in a number of human cancers, such as lung cancer, colorectal cancer, and multiple myeloma (4-6). Research studies have recently demonstrated that MAGE-A3 drives tumorigenesis as part of the MAGE-A3-TRIM28 ubiquitin ligase complex that promotes proteasomal degradation of the tumor suppressor kinase AMPK (7). Due to its upregulated and selective expression in human tumors and high degree of immunogenicity, MAGE-A3 has received significant attention as a novel immunotherapy target through the use of vaccines and adoptive cell therapy (8,9).

使用例
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
3900   Rabbit (DA1E) mAb IgG XP® Isotype Control
5127   Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate)
6883   SignalFire™ ECL Reagent
68978   MAGE-A10 Antibody
7003   20X LumiGLO® Reagent and 20X Peroxide
7071   Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
73778   Protein A Magnetic Beads
7727   Biotinylated Protein Ladder Detection Pack
8457   β-Actin (D6A8) Rabbit mAb
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

MAGE-A3 (E9S4X) Rabbit mAb

Metabolic Reprogramming in Disease

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