#40758 Phospho-CASC5 (Thr943/Thr1155) (D8D4N) Rabbit mAb
|Phospho-CASC5 (Thr943/Thr1155) (D8D4N) Rabbit mAb recognizes endogenous levels of CASC5 protein only when phosphorylated at Thr943 or Thr1155. The protein sequences surrounding these two sites are identical.|
|Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr943 of human CASC5 protein.|
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Western blot analysis of extracts from HT-29 and HeLa cells, untreated or treated with Nocodozole #2190 (100 ng/mL, 16 hr) to enrich cells in mitosis, using Phospho-CASC5 (Thr943/Thr1155) (D8D4N) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).
Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or post-processed with λ-phosphatase (right), using
Phospho-CASC5 (Thr943/Thr1155) (D8D4N) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
Kinetochores are mitotic structures that form on centromeres and attach to mitotic spindle microtubules. Kinetochore attachment to microtubules regulates chromosome segregation and progression through mitosis. Unattached kinetochores signal to the spindle assembly checkpoint (SAC) machinery, arresting cells in mitosis (1). CASC5, also known as Knl1 or Blinkin, is the largest subunit of the Knl1–Mis12–Ndc80 complex (KMN) network, a structural component of kinetochores required for microtubule binding. CASC5 functions in the formation of kinetochore–microtubule attachments, chromosome segregation, and in activating the SAC. CASC5 has been implicated in human diseases, including leukemia and microcephaly (2). Activation of the SAC is regulated in part by mitotic phosphorylation of CASC5 at several sites, including Ser24, Ser60, Thr943, and Thr1155 (3, 4). The sequences surrounding Thr943 and Thr1155 are identical.
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DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.