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#4087 Propidium Iodide (PI)/RNase Staining Solution

 
  • Propidium Iodide (PI)/RNase Staining Solution Data Sheet PDF

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CSTコード 包装
希望納入価格 (円)
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2019年1月18日15時10分 現在
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#4087S100 mL27,000
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製品概要
蛍光顕微鏡下の核染色やフローサイトメトリーを使用した細胞周期解析におけるDNA 量の測定に使用します。
貯法
4℃
社内データ

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-Histone H3 (Ser10) Antibody (Alexa Fluor® 488 Conjugate) #9708 and Propidium Iodide (PI)/RNase Staining Solution (DNA content).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb #3873 (green). Red = Propidium Iodide (PI)/RNase Staining Solution.

IF-F

IF-F

Confocal immunofluorescent analysis of rat cerebellum using S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (blue pseudocolor) and β3-Tubulin (D71G9) XP® Rabbit mAb #5568 (green). Red = Propidium Iodide (PI)/RNase Staining Solution.


バックグラウンド

Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is achieved by enzymatic removal of RNA with a ribonuclease (RNase). PI/RNase is commonly used as a nuclear stain in fluorescent microscopy and as a DNA content determinant in cell cycle analyses by flow cytometry. Cells in G2 and M phases of the cell cycle contain twice the DNA content compared to those in G0 and G1 phases. DNA content during S phase lies between these extremes. PI/RNase Staining Solution has excitation and emission maxima of 535 and 617 nm, respectively (orange to red range of the spectrum). This staining solution can be used in tandem with antibodies without binding interference and is a suitable DNA stain for multiplex assays. (1-3)

使用文献

Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Propidium Iodide (PI)/RNase Staining Solution

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