#41104 CD45 (30-F11) Rat mAb (APC Conjugate)
|内在性||Rat IgG2b, kappa||4℃|
|CD45 (30-F11) Rat mAb (APC Conjugate) recognizes endogenous levels of total mouse CD45 protein. This antibody detects an epitope within the extracellular domain.|
|This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.|
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Flow cytometric analysis of live mouse splenocytes using CD45 (30-F11) Rat mAb (APC Conjugate) (solid line) compared to concentration-matched Rat (LTF-2) mAb IgG2b Isotype Control (APC Conjugate) #34828 (dashed line).
The protein phosphatase (PTP) receptor CD45 is a type I transmembrane protein comprised of a pair of intracellular tyrosine phosphatase domains and a variable extracellular domain generated by alternative splicing (1). The catalytic activity of CD45 is a function of the first phosphatase domain (D1) while the second phosphatase domain (D2) may interact with and stabilize the first domain, or recruit/bind substrates (2,3). CD45 interacts directly with antigen receptor complex proteins or activates Src family kinases involved in the regulation of T- and B-cell antigen receptor signaling (1). Specifically, CD45 dephosphorylates Src-family kinases Lck and Fyn at their conserved negative regulatory carboxy-terminal tyrosine residues and upregulates kinase activity. Conversely, studies indicate that CD45 can also inhibit Lck and Fyn by dephosphorylating their positive regulatory autophosphorylation site. CD45 appears to be both a positive and a negative regulator that conducts signals depending on specific stimuli and cell type (1). Human leukocytes including lymphocytes, eosinophils, monocytes, basophils, and neutrophils express CD45, while erythrocytes and platelets are negative for CD45 expression (4).
The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets.
|34828 Rat (LTF-2) mAb IgG2b Isotype Control (APC Conjugate)|
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