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#42582 EOMES (D8D1R) Rabbit mAb (Alexa Fluor® 647 Conjugate)

 

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2019年3月22日15時35分 現在
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EOMES 製品一覧

感度抗体の由来貯法
内在性Rabbit IgG4℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

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42582 の推奨プロトコール i

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特異性・感度
EOMES (D8D1R) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total EOMES protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro180 of human EOMES protein.

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社内データ

※下記の社内データは、すべて42582 の推奨プロトコールで実験した結果です。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human peripheral blood mononuclear cells, co-stained with a CD14 antibody (left) or a CD3 antibody (right), using EOMES (D8D1R) Rabbit mAb (Alexa Fluor® 647 Conjugate) (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (bottom row).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of RPMI 8226 cells (blue) and NK-92 cells (green) using EOMES (D8D1R) Rabbit mAb (Alexa Fluor® 647 Conjugate) (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate) #2985 (dashed lines).

バックグラウンド

The T-box family of transcription factors is named for their shared homology with the DNA binding domain of the mouse brachyury (T) gene product. Members of this family bind DNA and are capable of transcriptional activation. They also have evolutionarily conserved expression patterns and roles in embryonic development, primarily mesoderm development (1). EOMES, or Tbr2 (T-box brain 2), is a master regulator of mesoderm formation that is also essential for trophoblast formation, gastrulation, neurogenesis and the differentiation of certain T cell subsets. Embryos from EOMES knock-out mice die soon after implantation due to their inability to develop a trophoblast (2,3). Conditional neural knock out mice show defects in development of a specific population of neural progenators known as Intermediate Progenator Cells (IPCs) that give rise only to neurons (4,5). These cells are formed from the radial glia in the ventricular and sub-ventricular zones of the cortex. Expression of EOMES increases as cells develop from radial glia to IPCs and then decreases as IPCs progress to neurons. Recent evidence suggests that EOMES and IPCs may also play a role neurogenesis in the adult hippocampal SGZ (5). EOMES is also a key transcription factor for memory T cells and for full effector differentiation of CD8+ T cells (6). Expression of EOMES is induced in CD8+ T cells following viral infection and bacterial infection where sufficient IL-12 has been produced to elicit acute host cell response (7).

使用例
 
関連製品
81493   EOMES (D8D1R) Rabbit mAb

XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

本製品は試験研究用です。

EOMES (D8D1R) Rabbit mAb (Alexa Fluor® 647 Conjugate)

Immune Cell Signaling Pathways

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