CST logo

#43248 PD-1 (EH33) Mouse mAb (IHC Specific)

Shopping Days対象製品 対象製品の中から、まとめて購入で一部製品が無料に!


CSTコード 包装
希望納入価格 (円)
Shopping Daysで購入
国内在庫 i
2019年3月26日15時45分 現在
ご登録代理店情報 i
#43248S100 μL89,000

PDCD1 XP®モノクローナル抗体 | PDCD1 製品一覧

内在性Mouse IgG2a-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト -
43248 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



免疫組織染色 (パラフィン) (1:200)Immunohistochemistry (Leica® Bond™) (1:100)


IHC-P: 抗体希釈液 / 抗原賦活化


ホルマリン固定したパラフィン包埋組織切片の免疫組織染色では内在性レベルのPD-1 タンパク質を検出します。ウェスタンブロッティングではトランスフェクトレベルのPD-1 タンパク質を検出します。
ヒトのPD-1 タンパク質のN末端特異的な組換えタンパク質

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて43248 の推奨プロトコールで実験した結果です。

IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using PD-1 (EH33) Mouse mAb (IHC-Specific) performed on the Leica® BOND™ Rx.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon (ulcerative colitis) using PD-1 (EH33) Mouse mAb (IHC-Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using PD-1 (EH33) Mouse mAb (IHC-Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded 293 cell pellets, control (left) or PD-1 transfected (right), using PD-1 (EH33) Mouse mAb (IHC-Specific).

IHC-P (paraffin)

IHC-P (paraffin)

Multiplex immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using PD-1 (EH33) mouse mAb (green), CD8α (C8/144B) mouse mAb #70306 (magenta), CD68 (D4B9C) XP® rabbit mAb #76437 (red), Pan-keratin (C11) mouse mAb #4545 (cyan), LAG3 (D2G4O™) XP® rabbit mAb #15372 (orange), and TIM-3 (D5D5R™) XP® rabbit mAb #45208 (yellow).


The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses (1-3). The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2 (4). Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling (5-7). In addition to activated T-cells, PD-1 is expressed in activated B-cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance (3); however, research studies show that cancer cells often adopt this pathway to escape immune surveillance (9). Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention (10).

14166   Hematoxylin
14177   SignalStain® Mounting Medium
14747   SignalStain® EDTA Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
6883   SignalFire™ ECL Reagent
7003   20X LumiGLO® Reagent and 20X Peroxide
7072   Phototope-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8125   SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.
Tween is a registered trademark of ICI Americas, Inc.
LEICA is a registered trade​mark of Leica Microsystems IR GmbH.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


PD-1 (EH33) Mouse mAb (IHC Specific)

Immune Cell Signaling Pathways